Device and method for solid phase extraction
    122.
    发明公开
    Device and method for solid phase extraction 审中-公开
    Gerätund Verfahren zur Festphasen-Extraktion

    公开(公告)号:EP1139087A2

    公开(公告)日:2001-10-04

    申请号:EP01301748.8

    申请日:2001-02-26

    IPC分类号: G01N1/34

    摘要: Sample preparation device and method for removing impurities and undesired matter from samples prior to further analysis such as by MALDI or electro-spray ionization (ESI). The sample preparation device, such as a standard pipette tip, has chromatography media bound to the inside surface of the tip so as to create a monolayer of particles that are readily accessible to fluids that flow through the pipette tip. The device can be used for washing, protein binding, elution processes, etc. A method of sample preparation using the device of the present invention is also disclosed.

    摘要翻译: 在进一步分析之前,例如通过MALDI或电喷雾电离(ESI),从样品中除去杂质和不期望物质的样品制备装置和方法。 样品制备装置,例如标准移液管尖端,具有与尖端的内表面结合的色谱介质,以便产生可以流过移液管尖端的流体容易接近的单层颗粒。 该装置可用于洗涤,蛋白质结合,洗脱过程等。还公开了使用本发明的装置的样品制备方法。

    Electrically insulated gas chromatograph assembly
    125.
    发明公开
    Electrically insulated gas chromatograph assembly 有权
    气相色谱仪气相色谱仪

    公开(公告)号:EP0962767A1

    公开(公告)日:1999-12-08

    申请号:EP99304352.0

    申请日:1999-06-03

    IPC分类号: G01N30/30

    摘要: An electrically insulated gas chromatograph assembly suitable for high temperature operation in a miniaturized, low power, low thermal mass gas chromatograph instrument is provided. The gas chromatograph (GC) assembly includes a metal capillary GC column (152) having a ceramic fiber insulating layer (156) wrapped about an outer surface thereof. Heater wire (160) is similarly wrapped with a ceramic fiber electrical insulating layer (158). Resistive temperature device (RTD) wire (154) is positioned contiguous an outer surface of insulating layer (156). The composite assembly including insulated capillary GC column (152), insulated heater wire (160), and RTD wire (154), are bound together by applying thereto a spiral wrapped ceramic fiber insulating layer (162).

    摘要翻译: 提供了一种适用于小型,低功率,低热质量气相色谱仪器的高温运行的电绝缘气相色谱仪组件。 气相色谱仪(GC)组件包括金属毛细管GC柱(152),其具有围绕其外表面缠绕的陶瓷纤维绝缘层(156)。 加热线(160)类似地用陶瓷纤维电绝缘层(158)包裹。 电阻温度装置(RTD)导线(154)邻近绝缘层(156)的外表面定位。 包括绝缘毛细管GC柱(152),绝缘加热丝(160)和RTD丝(154)的复合组件通过施加螺旋缠绕的陶瓷纤维绝缘层(162)而结合在一起。

    METHOD AND SILICATE COMPOSITION FOR CONDITIONING SILICA SURFACES
    126.
    发明公开
    METHOD AND SILICATE COMPOSITION FOR CONDITIONING SILICA SURFACES 失效
    方法和组合物SILIKAT空调二氧化硅曲面的

    公开(公告)号:EP0835443A1

    公开(公告)日:1998-04-15

    申请号:EP96924383.0

    申请日:1996-07-10

    IPC分类号: G01N27 G01N30

    摘要: Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surfaces(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li?+, Na+, or K+¿. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.

    Liquid chromatograph and liquid chromatography
    128.
    发明公开
    Liquid chromatograph and liquid chromatography 失效
    ChromatographfürdieFlüssigkeitschromatographie

    公开(公告)号:EP0763734A2

    公开(公告)日:1997-03-19

    申请号:EP96114466.4

    申请日:1996-09-10

    申请人: HITACHI, LTD.

    IPC分类号: G01N30/96

    摘要: The invention intends to provide a liquid chromatograph and chromatography which can perform analysis at a higher speed with clearer separation between peaks. The liquid chromatograph comprises a separation column (10), a buffer pump (7) for feeding a plurality of buffers 1, 2, 3 and 4 into the separation column (10), a sampler (9) disposed in a flow path between the buffer pump (7) and the separation column (10) for introducing a sample to be analyzed into the flow path, and a reaction coil (14) for mixing and then reacting amino acids separated by the separation column (10) with a ninhydrin reagent. The separation column (10) is sized so that a ratio (L1/R) of its length L1 to its inner diameter R is not more than 10. The particle size of ion exchange resin filled in the separation column (10) is selected to be not more than 4 µm.

    摘要翻译: 本发明旨在提供一种液相色谱和色谱法,其可以以更高的速度进行分析,并在峰之间更清晰地分离。 液相色谱仪包括分离塔(10),用于将多个缓冲液1,2,3和4供给到分离塔(10)中的缓冲泵(7),一个采样器(9) 缓冲泵(7)和用于将要分析的样品引入流路的分离塔(10)和用于混合然后使由分离塔(10)分离的氨基酸与茚三酮试剂反应的反应线圈(14) 。 分离柱(10)的尺寸使其长度L1与其内径R的比(L1 / R)不大于10.填充在分离塔(10)中的离子交换树脂的粒径选择为 不超过4亩。

    Chromatographiesäule
    129.
    发明公开
    Chromatographiesäule 失效
    Verfahren zum Herstellen einerChromatographiesäule

    公开(公告)号:EP0762118A2

    公开(公告)日:1997-03-12

    申请号:EP96114601.6

    申请日:1996-09-12

    申请人: K.D. PHARMA GmbH

    IPC分类号: G01N30/00 B01D15/08

    摘要: Eine Chromatographiesäule besteht aus einem fest installierten Kolbenzylinder (1) von mindestens 200 mm Durchmesser und z.B. 1000 bis 2000 mm Länge,

    der in dem einen Zylinderraum die Packung (33) der Chromatographiesäule enthält und in dem anderen, von der Kolbenstange (5) durchsetzten Zylinderraum mit einem Druckmedium gefüllt ist,
    in dem die Zuleitung für den Eluenten durch die Kolbenstange (5) un den Kolben (4) hindurch geführt ist und die Ableitung für das Eluat durch die betreffende Zylinderstirnwand (2) hindurch geführt ist oder umgekehrt
    und in dem der mit dem Druckmedium gefüllte Zylinderraum an eine Druckquelle solcher Stärke angeschlossen ist, daß das Druckmedium auf den Kolben (4) eine Kraft ausübt, mit der dieser die Packung (33) zusammenhält.
    Alle Kräfte werden hier innerhalb des Kolbenzylinders ausgeglichen und nicht über ein, bisher übliches, Untergestell. Es sind Drücke möglich bis zu 320 bar und mehr bei z.B. 800 mm Durchmesser.
    Der Kolbenzylinder (1) ist aus einem hydraulischen Druckspeicher hergestellt durch Umgestaltung des Kolbens (4), der Stirnwand (2) des die Packung (33) enthaltenden Zylinderraumes und der, hohlen, Kolbenstange (5).

    摘要翻译: 色谱柱由活塞扫过的圆筒组成。 新颖的是,(a)气缸室的一部分包含填料(33),而在活塞另一侧的腔室的另一部分并保持活塞杆,填充有加压介质; (b)洗脱液的入口通过活塞杆(5)和活塞(4),而洗脱液的出口通过适当的气缸面(2),反之亦然; (c)围绕活塞杆的体积的介质被加压至一个程度,使得其在活塞(4)上施加力,通过该活塞将所述包装(33)保持在一起。 气缸(1)由预制件制成。 通过重新设计容纳活塞(4),端面(2)和填料(33)的腔室以及中空活塞杆(5),加压液压容器。