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21 条记录 (耗时 0.016 秒)

    Use of Valproate for reducing CHOP levels, particularly in a mammal having SCI
    11.
    发明公开
    Use of Valproate for reducing CHOP levels, particularly in a mammal having SCI 审中-公开
    Verwendung von Valproat zur Reduktion der CHOP-Spiegel，insbesondere ein einemSäugetiermit SCI

    公开(公告)号：EP2181708A1

    公开(公告)日：2010-05-05

    申请号：EP08382062.1

    申请日：2008-10-31

    申请人： Universitat Autonoma de Barcelona

    发明人： Casas Louzao, Catalina ,  Penas Pérez, Clara ,  Navarro Acebes, Xavier ,  Verdu Navarro, Enric

    IPC分类号： A61K31/19 ,  A61P25/00

    CPC分类号： A61K31/19

    摘要： The present invention relates to the use of valproate in the manufacture of a drug for reducing CHOP levels in a mammal. In particular, said mammal has spinal cord injury (SCI), particularly traumatic SCI. The present invention, therefore, also relates to the use of valproate in the manufacture of a drug for treating SCI in a mammal, particularly traumatic SCI. The present invention also relates to a method for reducing CHOP levels in a mammal, particularly said mammal having spinal cord injury (SCI), specifically traumatic SCI. The present invention also relates to valproate for use in reducing CHOP levels in a mammal, particularly said mammal having spinal cord injury (SCI), specifically traumatic SCI.

    摘要翻译： 本发明涉及丙戊酸盐在制备用于降低哺乳动物的CHOP水平的药物中的用途。 特别地，所述哺乳动物具有脊髓损伤（SCI），特别是创伤性SCI。 因此，本发明还涉及丙戊酸盐在制备用于治疗哺乳动物，特别是创伤性SCI中的SCI的药物中的用途。 本发明还涉及一种用于降低哺乳动物，特别是所述具有脊髓损伤（SCI）的哺乳动物，特别是外伤性SCI的CHOP水平的方法。 本发明还涉及用于降低哺乳动物，特别是所述具有脊髓损伤（SCI）的哺乳动物，特别是创伤性SCI的CHOP水平的丙戊酸盐。

    HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR
    16.
    发明公开
    HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR 有权
    HLA-B基因组突变体免疫球蛋白基因

    公开(公告)号：EP1964929A1

    公开(公告)日：2008-09-03

    申请号：EP06841799.7

    申请日：2006-12-12

    申请人： Universitat Autonoma de Barcelona ,  Banc De Sang I Teixits

    发明人： JUAN OTERO, Manuel ,  CASAMITJANA PONCES, Natàlia ,  PALOU RIVERA, Eduardo ,  PUJOL BORRELL, Ricardo ,  FANER CANET, María, Rosa

    IPC分类号： C12Q1/68 ,  G01N33/58

    CPC分类号： C12Q1/6881 ,  C12Q2600/156

    摘要： Method and kit based on real-time PCR with specific primers and probes that allow to achieve a high degree of subtyping of the complete HLA-B locus. The main advantages are the greater speed (65 minutes, including the interpretation); the ease of automation, since only eighteen tubes are necessary to obtain a good level of resolution (typing of 300 groups); reduction of the total cost per test thanks to the ease of automation and the simplicity; a surprisingly high degree of allele definition is achieved; and the risk of sample contamination is reduced because the amplified products always remain in the tubes and no post-PCR steps are necessary.

    摘要翻译： 基于实时PCR的特异性引物和探针的方法和试剂盒，其允许实现完整的HLA-B基因座的高度亚型化。 主要优点是速度更快（65分钟，包括解释）; 自动化的便利性，因为只有十八根管是获得良好的分辨率（打300个组）所必需的; 由于易于自动化和简单性，每次测试的总成本降低; 达到惊人的高度等位基因定义; 并且样品污染的风险降低，因为扩增产物总是保留在管中，并且不需要后PCR步骤。

    Telomerase reverse transcriptase for protection against ageing
    17.
    发明公开
    Telomerase reverse transcriptase for protection against ageing 有权
    端粒酶Umkehrtranskriptase zum Schutz vor Alterung

    公开(公告)号：EP2402038A1

    公开(公告)日：2012-01-04

    申请号：EP10168341.5

    申请日：2010-07-02

    申请人： Fundación Centro Nacional De Investigaciones Oncológicas Carlos III ,  Universitat Autonoma de Barcelona

    发明人： Blasco Marhuenda, María Antonia ,  Bernardes, Bruno ,  Bosch Tubert, Fátima ,  Ayuso, Eduard

    IPC分类号： A61K48/00 ,  C12N15/864 ,  A61P43/00

    CPC分类号： A61K48/005 ,  C12N15/86 ,  C12N2750/14143

    摘要： The invention relates to expression vectors of the telomerase reverse transcriptase, especially non integrative vectors such as those derived from adeno-associated viruses (AAV), preferably derived from AAV9, as well as to its use for the amelioration of markers of ageing such as insulin insensitivity, osteoporosis, loss of neuromuscular coordination, loss of memory, and the longevity itself. It is based on the fact that treatment of 1-yr and 2-yr old mice with an adeno associated virus (AAV) of wide tropism expressing telomerase had remarkable beneficial effects on health and fitness, including the mentioned markers of ageing and the lifespan itself. Importantly, telomerase-treated mice did not develop more cancer than their control littermates, So, these results demonstrate that telomerase-based therapies can be used to improve healthy ageing and extend longevity in an adult/old mammalian organism without increasing the risk of cancer.

    摘要翻译： 本发明涉及端粒酶逆转录酶的表达载体，特别是非整合载体，例如衍生自AAV9的腺相关病毒（AAV）的那些，以及其用于改善老化标志物如胰岛素的用途 不敏感，骨质疏松，神经肌肉协调丧失，记忆丧失和长寿本身。 这是基于以下事实：用广泛向性表达端粒酶的腺相关病毒（AAV）治疗1岁和2岁的老鼠对健康和健康有显着的有益影响，包括所提及的老化标记和寿命本身 。 重要的是，端粒酶治疗的小鼠没有比对照的同窝出血发展更多的癌症。因此，这些结果表明基于端粒酶的疗法可用于改善健康老化并延长成年/老年哺乳动物生物体的寿命，而不增加癌症的风险。

    METHOD FOR PRODUCING ADENOVIRUS VECTORS FOR GENE THERAPY AND DNA SEQUENCES USED THEREFOR
    18.
    发明公开
    METHOD FOR PRODUCING ADENOVIRUS VECTORS FOR GENE THERAPY AND DNA SEQUENCES USED THEREFOR 审中-公开
    PROCESS FOR腺病毒载体用于基因治疗的生产，确保使用的DNA序列

    公开(公告)号：EP2020436A1

    公开(公告)日：2009-02-04

    申请号：EP07765843.3

    申请日：2007-04-27

    申请人： Universitat Autonoma de Barcelona ,  Institució Catalana de Recerca i Estudis Avançats

    发明人： CHILLÓN RODRÍGUEZ, Miguel ,  ALBA FERNÁNDEZ, Raúl ,  BOSCH MERINO, Assumpció

    IPC分类号： C12N7/02 ,  C12N15/861

    CPC分类号： C12N15/86 ,  A61K48/0091 ,  C12N7/00 ,  C12N2710/10343

    摘要： Method for producing adenovirus vectors for gene therapy and auxiliary vectors used therefor. The method is based on the multiplication of gutless adenoviruses that lack adenovirus-coding sequences by cotransfecting them with an auxiliary or helper adenovirus that has an attB sequence of the &phis;C31 bacteriophage inserted between the adenovirus packaging signal and the ITR closest to it and/or utilizing the delay arising at the time of packaging the helper adenovirus with respect to that of the gutless adenovirus owing to the presence of the atttB sequence in order to recover the gutless adenovirus from the culture before the helper adenovirus completes its viral cycle. This gives rise to high gutless adenovirus titres that are essentially free from helper adenovirus, thereby allowing them to be used in gene therapy, minimizing the likelihood of the appearance of a cellular immune response on the part of the treated individual against cells transduced by the adenovirus vector produced.

    摘要翻译： 的制造腺病毒载体用于基因治疗和辅助矢量方法为此使用。 该方法是基于无肠腺病毒的增殖没有通过共转染themwith到辅助或辅助腺病毒油漆腺病毒编码序列确实具有attB位的序列＆PHIS;腺病毒包装信号和ITR最接近它之间插入C31噬菌体和/ 或利用在延迟相对于所述无肠腺病毒的包装辅助性腺病毒的时间所产生做，以便在辅助腺病毒完成其病毒周期之前从培养回收无肠腺病毒由于atttB序列的存在的。 这产生了高无肠腺病毒的滴度确实是从辅助腺病毒基本上不含，从而使他们能够在基因治疗中使用，最小化对治疗的个体的一部分的细胞免疫应答抵抗由腺病毒转导的细胞的外观的可能性 载体产生。

    METHOD AND KIT FOR THE GENOTYPIFICATION OF HLA-DRB BASED ON REAL TIME PCR
    19.
    发明公开
    METHOD AND KIT FOR THE GENOTYPIFICATION OF HLA-DRB BASED ON REAL TIME PCR 审中-公开
    VERFAHREN UND KIT ZUR GENOTYPISIERUNG VON HLA-DRB AUF ECHTZEIT-PCR-BASIS

    公开(公告)号：EP1743946A1

    公开(公告)日：2007-01-17

    申请号：EP04805141.1

    申请日：2004-12-03

    申请人： UNIVERSITAT AUTONOMA DE BARCELONA

    发明人： JUAN OTERO, Manuel, LIRAD - SSR-CTBT ,  CASAMITJANA PONCES, Natàlia, LIRAD - SSR-CTBT ,  PUJOL BORRELL, Ricardo, LIRAD - SSR-CTBT ,  COLOBRAN ORIOL, Roger, LIRAD - SSR-CTBT ,  PALOU RIVERA, Eduardo, LIRAD - SSR-CTBT ,  FANER CANET, Maria Rosa, LIRAD - SSR-CTBT ,  RIBERA CRUSAFONT, Ana, LIRAD - SSR-CTBT

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6881 ,  C12Q1/686 ,  C12Q2600/156

    摘要： Method for determining the genotype of loci DRB of human leukocyte antigen from a nucleic acid sample, and kit for performing this method. The kit comprises allele-specific primers for amplifying nucleic acids derived from HLA-DRB loci by real-time PCR; allele-specific fluorescently labelled nucleotide probes for detecting fluorescence signals during the amplification; and experimentally defined fluorescence patterns for comparing the detected signals. In particular the genes of DRB loci are selected from DRB1, DRB3, DRB4 and DRB5. It overcomes some of the limitations of conventional DNA-based typing methods, in terms of time, possibility of automation, and increase in resolution, while reducing the number of PCR reactions. It is useful e.g. for determining transplantation compatibility or susceptibility for a specific disease.

    摘要翻译： 用于确定来自核酸样品的人白细胞抗原基因座DRB的基因型的方法和用于进行该方法的试剂盒。 该试剂盒包括通过实时PCR扩增从HLA-DRB基因座衍生的核酸的等位基因特异性引物; 用于在扩增期间检测荧光信号的等位基因特异性荧光标记核苷酸探针; 以及用于比较检测到的信号的实验定义的荧光图案。 特别地，DRB基因座的基因选自DRB1，DRB3，DRB4和DRB5。 克服了传统的基于DNA的打字方法的一些局限性，从时间，自动化的可能性和分辨率的提高，同时减少了PCR反应的数量。 这是有用的 用于确定特定疾病的移植相容性或易感性。