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21 条记录 (耗时 0.031 秒)

    CRISPR-BASED COMPOSITIONS AND METHODS OF USE
    11.
    发明公开
    CRISPR-BASED COMPOSITIONS AND METHODS OF USE 审中-公开
    基于CRISPR的组合物和使用方法

    公开(公告)号：EP3234133A2

    公开(公告)日：2017-10-25

    申请号：EP15828610.4

    申请日：2015-12-18

    申请人： Integrated DNA Technologies, Inc.

    发明人： COLLINGWOOD, Michael, Allen ,  JACOBI, Ashley, Mae ,  RETTIG, Garrett, Richard ,  SCHUBERT, Mollie, Sue ,  BEHLKE, Mark, Aaron

    IPC分类号： C12N15/11

    摘要： This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.

    摘要翻译： 本发明涉及用于CRISPR系统的改性组合物及其使用方法。 具体而言，长度修饰和化学修饰形式的crRNA和tracrRNA被描述用作重构的指导RNA以与CRIPSR系统的Cas9相互作用。 得到的长度修饰和化学修饰形式的crRNA和tracrRNA经济地生产，并且可以定制为在CRIPSR Cas9内切核酸酶系统的背景下具有与其生物化学和生物学活性相关的独特性质。

    METHODS AND SYSTEMS FOR ESTIMATING THE MELTING TEMPERATURE (Tm) FOR POLYNUCLEOTIDE MOLECULES
    14.
    发明授权
    METHODS AND SYSTEMS FOR ESTIMATING THE MELTING TEMPERATURE (Tm) FOR POLYNUCLEOTIDE MOLECULES 有权
    METHOD AND SYSTEM FOR FOR多核苷酸估算的熔融温度（T M）

    公开(公告)号：EP1543438B1

    公开(公告)日：2009-05-06

    申请号：EP03754518.3

    申请日：2003-09-12

    申请人： INTEGRATED DNA TECHNOLOGIES, INC.

    发明人： OWCZARZY, Richard ,  WALDER, Joseph, Alan ,  HUANG, Lingyan ,  BEHLKE, Mark, Aaron

    IPC分类号： G06F17/00 ,  G06F17/10 ,  G06F17/11 ,  C12Q1/68

    CPC分类号： G06F19/20 ,  C12Q1/6813 ,  G06F19/22

    摘要： The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, particularly duplexes of oligonucleotides which may be used, for example, as primers or probes in PCR and/or hybridization assays. The invention also relates to methods and systems for designing and selecting oligonucleotide probes and primers having a predicted melting temperature which is optimized for such assays. To this end, algorithms and methods are provided for predicting the melting temperature of a nucleic acid having a predetermined sequence. These methods and algorithms estimate the melting temperature of a nucleic acid duplex under particular salt conditions. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of particular salt conditions on the melting temperature. As such, the methods and systems of the invention provide superior result compared to existing methods, which do not consider sequence dependent effects of changing salt conditions. ­

    METHODS FOR AMPLIFYING POLYMERIC NUCLEIC ACIDS
    19.
    发明授权
    METHODS FOR AMPLIFYING POLYMERIC NUCLEIC ACIDS 有权
    方法扩增高分子NUCLEIC

    公开(公告)号：EP1649065B1

    公开(公告)日：2010-03-03

    申请号：EP04779936.6

    申请日：2004-08-02

    申请人： INTEGRATED DNA TECHNOLOGIES, INC.

    发明人： BEHLKE, Mark, Aaron ,  WALDER, Joseph, Alan ,  MANTHEY, Jeffrey, A.

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2531/101 ,  C12Q2525/186 ,  C12Q2525/121 ,  C12Q2531/113

    摘要： The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer. It is preferred that the non-replicable primer hybridizes to the nucleic acid polymer and is extended to produce an extension product that contains sequence from the nucleic acid polymer to which the replicable primer then hybridizes. Of course, if the nucleic acid polymer is double stranded, both the replicable and nonreplicable primers will hybridize and be extended by DNA polymerase.