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18 条记录 (耗时 0.016 秒)

    PROCÉDÉ DE DÉTERMINATION DE LA CHARGE MUTATIONNELLE D'UNE BANQUE DE GÈNES OBTENUE PAR MUTAGÉNÈSE ALÉATOIRE D'UN GÈNE D'INTÉRÊT ET LES MOYENS POUR SA MISE EN OEUVRE
    11.
    发明公开
    PROCÉDÉ DE DÉTERMINATION DE LA CHARGE MUTATIONNELLE D'UNE BANQUE DE GÈNES OBTENUE PAR MUTAGÉNÈSE ALÉATOIRE D'UN GÈNE D'INTÉRÊT ET LES MOYENS POUR SA MISE EN OEUVRE 有权
    用于确定突变是随机突变特定基因获得的基因库的程度程序以及将它们实现

    公开(公告)号：EP1774032A2

    公开(公告)日：2007-04-18

    申请号：EP05775328.7

    申请日：2005-06-07

    申请人： Proteus

    发明人： CHODORGE, Matthieu ,  FOURAGE, Laurent ,  LEFEVRE, Fabrice ,  MASSON, Jean-Michel

    IPC分类号： C12Q1/68 ,  G06F1/00

    CPC分类号： C12N15/102 ,  G06F19/22

    摘要： The invention relates to a method of determining the mutational load of a gene library obtained by random mutagenesis of a particular gene of interest and means for implementing same, comprising the following steps: a) preparation of an abacus that links the mutational load (CM) of a gene library obtained by random mutagenesis of a model gene to the fraction of mutated model genes observed in said library; b) random mutagenesis of the model gene that was used to prepare the abacus in step (a) and of the gene of interest, in order to produce the corresponding mutated gene libraries; c) determination of the mutational load (CM) of the gene library obtained from the model gene in step (b) using the abacus established in step (a); and d) application of a correction factor (CF) to the mutational load (CM) of the mutated model gene library determined in step (C), in order to determine the mutational load (CM) of the library of mutated genes of interest from step (b).

    METHOD OF PREPARING POLYNUCLEOTIDE FRAGMENTS FOR USE IN SHUFFLING
    12.
    发明公开
    METHOD OF PREPARING POLYNUCLEOTIDE FRAGMENTS FOR USE IN SHUFFLING 有权
    用于生产多核苷酸片段FOR USE IN洗牌

    公开(公告)号：EP1404830A2

    公开(公告)日：2004-04-07

    申请号：EP02743542.9

    申请日：2002-05-16

    申请人： Proteus

    发明人： DUPRET, Daniel ,  LEFEVRE, Fabrice ,  FOURAGE, Laurent

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1027

    摘要： Method of preparing fragments for shuffling using DNA repair enzymes. Repair enzymes can be used to repair damaged and mismatched residues to reduce level of mutation in fragments, or can themselves create fragments when used without sufficient DNA polymerase. Invention further includes reaction mixtures created by or during the method, sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.

    PROCEDE DE SEPARATION ET DE CARACTERISATION DES FONCTIONS POTENTIELLEMENT PRESENTES DANS UN ECHANTILLON BIOLOGIQUE CONTENANT DES ACIDES NUCLEIQUES
    17.
    发明公开
    PROCEDE DE SEPARATION ET DE CARACTERISATION DES FONCTIONS POTENTIELLEMENT PRESENTES DANS UN ECHANTILLON BIOLOGIQUE CONTENANT DES ACIDES NUCLEIQUES 有权
    进行隔离势函数的表征方法对生物样品的基础上，它包含NUCLEIC

    公开(公告)号：EP1104489A1

    公开(公告)日：2001-06-06

    申请号：EP99936724.6

    申请日：1999-08-11

    申请人： Proteus S.A.

    发明人： DUPRET, Daniel ,  MASSON, Jean-Michel ,  LEFEVRE, Fabrice

    IPC分类号： C12Q1/68 ,  C12N15/00 ,  C12N15/63

    CPC分类号： C12Q1/68

    摘要： The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.