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公开(公告)号：EP0977035B2
公开(公告)日：2010-07-21
申请号：EP99113781.1
申请日：1996-06-06
IPC分类号： G01N33/52 , G01N33/50 , C07D239/60 , C07D311/16 , C07D209/08 , C07F5/00 , C07F5/02
CPC分类号： G01N33/582 , C07D209/08 , C07D239/60 , C07D239/62 , C07D239/66 , C07D311/16 , C07F5/003 , C07F5/02 , C07F5/027 , C07F9/10 , C07F9/5728 , C09B23/02 , G01N33/5005 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/5044 , G01N33/5047 , G01N33/5058 , G01N33/5061 , G01N33/5076 , G01N33/52 , G01N33/542 , G01N33/6872 , G01N2500/10 , Y02P20/55 , Y10S436/805
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公开(公告)号：EP0982398B1
公开(公告)日：2003-11-05
申请号：EP99118473.0
申请日：1996-03-20
发明人： Tsien, Roger Y. , Zlokarnik, Gregor
CPC分类号： C07D501/36 , C07D501/00 , C07D505/00 , C07D519/00 , C12N9/86 , C12N15/85 , C12N2800/90 , C12N2830/002 , C12P17/184 , C12P17/188 , C12P35/00 , C12Q1/18 , C12Q1/34 , C12Q1/6897 , C12Q2334/00 , G01N2333/986 , G01N2415/00 , Y10S435/805
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13.发明公开Scanning confocal microscope providing a continuous display 失效Konfokales Rastermikroskop mit kontinuierlicher Anzeige。
公开(公告)号：EP0592089A2
公开(公告)日：1994-04-13
申请号：EP93306533.6
申请日：1993-08-18
发明人： Tsien, Roger Y.
CPC分类号： G02B21/008 , G02B21/0076
摘要： A scanning confocal microscope scans a sample with an incident beam of radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation in at least two wavelengths. A portion of the fluorescent light retraces a portion of the path optical of incident beam, to a dichroic mirror that separates it from the incident beam for detection by a pair of photomultiplier tubes. A data processor accumulates digital data from the photomultiplier tubes to form a succession of image data frames of the sample being scanned, in the two wavelengths. Image data for a selected number of frames in each wavelength is averaged and then recorded on a single track of a recording system, in an alternating fashion with averaged data for the other wavelength. In addition, a ratio of the averaged data for the two wavelengths is delivered to a video display.
摘要翻译： 扫描共焦显微镜以光栅扫描图案用入射光束扫描样品，导致样品在至少两个波长内发出可见光并发出可见光。 荧光的一部分将入射光束的光路的一部分返回到分光镜，该分色镜将其与入射光束分离，以便由一对光电倍增管检测。 数据处理器从光电倍增管累积数字数据，以在两个波长内形成扫描样本的一系列图像数据帧。 对每个波长中所选择的帧数的图像数据进行平均化，然后以与另一波长的平均数据交替的方式记录在记录系统的单个轨道上。 此外，两个波长的平均数据的比率被传送到视频显示器。
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公开(公告)号：EP1610126B1
公开(公告)日：2014-04-30
申请号：EP05015762.7
申请日：1996-06-06
IPC分类号： G01N33/50
CPC分类号： G01N33/582 , C07D209/08 , C07D239/60 , C07D239/62 , C07D239/66 , C07D311/16 , C07F5/003 , C07F5/02 , C07F5/027 , C07F9/10 , C07F9/5728 , C09B23/02 , G01N33/5005 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/5044 , G01N33/5047 , G01N33/5058 , G01N33/5061 , G01N33/5076 , G01N33/52 , G01N33/542 , G01N33/6872 , G01N2500/10 , Y02P20/55 , Y10S436/805
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公开(公告)号：EP1610126A1
公开(公告)日：2005-12-28
申请号：EP05015762.7
申请日：1996-06-06
IPC分类号： G01N33/50
CPC分类号： G01N33/582 , C07D209/08 , C07D239/60 , C07D239/62 , C07D239/66 , C07D311/16 , C07F5/003 , C07F5/02 , C07F5/027 , C07F9/10 , C07F9/5728 , C09B23/02 , G01N33/5005 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/5044 , G01N33/5047 , G01N33/5058 , G01N33/5061 , G01N33/5076 , G01N33/52 , G01N33/542 , G01N33/6872 , G01N2500/10 , Y02P20/55 , Y10S436/805
摘要： Methods and compositions are provided for determining the potential of a membrane. In one aspect, the method comprises: (a) introducing a first reagent comprising a hydrophobic fluorescent ion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane, as described by the Nernst equation, (b) introducing a second reagent which labels the first face or the second face of the membrane, which second reagent comprises a chromophore capable of undergoing energy transfer by either (i) donating excited state energy to the fluorescent ion, or (ii) accepting excited state energy from the fluorescent ion, (c) exposing the membrane to radiation; (d) measuring energy transfer between the fluorescent ion and the second reagent, and (e) relating the energy transfer to the membrane potential. Energy transfer is typically measured by fluorescence resonance energy transfer. In some embodiments the first and second reagents are bound together by a suitable linker. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.
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公开(公告)号：EP0977035B1
公开(公告)日：2005-09-14
申请号：EP99113781.1
申请日：1996-06-06
IPC分类号： G01N33/52 , G01N33/50 , C07D239/60 , C07D311/16 , C07D209/08 , C07F5/00 , C07F5/02
CPC分类号： G01N33/582 , C07D209/08 , C07D239/60 , C07D239/62 , C07D239/66 , C07D311/16 , C07F5/003 , C07F5/02 , C07F5/027 , C07F9/10 , C07F9/5728 , C09B23/02 , G01N33/5005 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/5044 , G01N33/5047 , G01N33/5058 , G01N33/5061 , G01N33/5076 , G01N33/52 , G01N33/542 , G01N33/6872 , G01N2500/10 , Y02P20/55 , Y10S436/805
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17.发明公开Substrates for beta-lactamase and uses thereof 失效Nukleinsäurenund deren Verwendung zur Bestimmung von Beta-Laktamase
公开(公告)号：EP0982398A1
公开(公告)日：2000-03-01
申请号：EP99118473.0
申请日：1996-03-20
发明人： Tsien, Roger Y. , Zlokarnik, Gregor
CPC分类号： C07D501/36 , C07D501/00 , C07D505/00 , C07D519/00 , C12N9/86 , C12N15/85 , C12N2800/90 , C12N2830/002 , C12P17/184 , C12P17/188 , C12P35/00 , C12Q1/18 , C12Q1/34 , C12Q1/6897 , C12Q2334/00 , G01N2333/986 , G01N2415/00 , Y10S435/805
摘要： The invention provides recombinant nucleic acid molecules comprising expression control sequences adapted for function in a vertebrate cell operably linked to a nucleotide sequence coding for the expression of beta-lactamase. Further, this invention concerns methods for determining the amount of beta-lactamase activity in a cell.
摘要翻译： 本发明提供重组核酸分子，其包含适于在编码β-内酰胺酶表达的核苷酸序列可操作地连接的脊椎动物细胞中的功能的表达调控序列。 此外，本发明涉及确定细胞中β-内酰胺酶活性的量的方法。
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公开(公告)号：EP1081495B1
公开(公告)日：2004-03-10
申请号：EP99117221.4
申请日：1999-09-01
发明人： Knapp, Tom , Zlokarnik, Gregor , Negulescu, Paul , Tsien, Roger Y. , Rink, Tim
IPC分类号： G01N33/542
CPC分类号： G01N33/542
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