公开(公告)号：EP2471908A1

公开(公告)日：2012-07-04

申请号：EP10811643.5

申请日：2010-07-28

申请人： The University of Tokyo ,  Mitsubishi Chemical Medience Corporation

发明人： MATSUI, Hitoshi ,  SAKAI, Yasuyuki ,  FUJII, Teruo ,  TAKEUCHI, Shoji ,  TSUDA, Yukiko

IPC分类号： C12N5/077 ,  C12M1/34 ,  C12Q1/02

CPC分类号： C12M23/20 ,  C12M23/24 ,  C12M25/14 ,  C12M41/46 ,  C12N5/0671 ,  C12N2503/00 ,  C12N2533/30 ,  C12N2533/54 ,  G01N33/5044

摘要： A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.

摘要翻译： 培养肝细胞的方法，其中将包埋在细胞外基质中的肝细胞置于透气膜上，并且在从透气膜侧供给氧的同时培养肝细胞。 由此，可以诱导肝细胞中的极性，并且可以在短时间内形成胆小管。 此外，所形成的极性可以保持更长的时间。

公开(公告)号：EP2241614A1

公开(公告)日：2010-10-20

申请号：EP09700562.3

申请日：2009-01-09

申请人： The University of Tokyo

发明人： TAKEUCHI, Shoji ,  ONOE, Hiroaki

IPC分类号： C12M3/00 ,  C12M1/14 ,  C12M1/40 ,  C12Q1/00 ,  C12N5/06

CPC分类号： C12M25/08 ,  B01L3/502761 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/0668 ,  B01L2300/0819

摘要： Provided is a technique for handling a minor amount of a biological material with the purpose of observing selectively the interaction between single cells by controlling cell number and two- or three-dimensional configurations of each of the different cell types. The present invention provides an instrument and a method for handling a minor amount of a biological material. The instrument of the present invention for handling a minor amount of a biological material comprises a construct and a base, wherein: the base holds the construct in a releasable manner; the construct is held on a surface of the base in such a manner that a part of the construct is exposed externally on the outer face of the instrument; and at least a part of a surface of the constructs is exposed externally on the outer face of the instrument being fabricated so as to allow the adhesion of the minor amount of the biological material thereto.

摘要翻译： 提供了一种处理少量生物材料的技术，其目的是通过控制每种不同细胞类型的细胞数量和二维或三维构型来选择性地观察单细胞之间的相互作用。 本发明提供一种用于处理少量生物材料的仪器和方法。 用于处理少量生物材料的本发明的仪器包括构建体和基底，其中：所述基座以可释放的方式保持构造; 将构造体保持在基部的表面上，使得构造体的一部分在仪器的外表面外部暴露; 并且构造物的表面的至少一部分在外部被暴露在被制造的器械的外表面上，以便允许少量的生物材料粘附到其上。

公开(公告)号：EP4083195A1

公开(公告)日：2022-11-02

申请号：EP20904571.5

申请日：2020-12-24

申请人： The University of Tokyo

发明人： TAKEUCHI, Shoji ,  MORIMOTO, Yuya ,  JO, Byeongwook ,  NIE, Minghao ,  TAJIMA, Ai

IPC分类号： C12N5/077

摘要： A method of producing a 3D tissue composite, comprising: a preparation step in which a multiple number of sheet-shaped first structures containing first cells are prepared, wherein at least one of the multiple number of first structures holds a second structure containing second cells; a stacking step in which the multiple number of first structures are stacked to form a 3D composite; and a culturing step in which the 3D composite is cultured to form a 3D tissue composite containing first tissues formed from the first cells and second tissues formed from the second cells.

公开(公告)号：EP3147346A1

公开(公告)日：2017-03-29

申请号：EP15796411.5

申请日：2015-05-20

申请人： The University of Tokyo

发明人： TAKEUCHI, Shoji ,  ONOE, Hiroaki ,  MIURA, Shigenori

IPC分类号： C12M1/00

CPC分类号： C12M25/10 ,  B01D69/081 ,  B01D69/085 ,  B01D69/12 ,  B01D69/144 ,  B01D71/08 ,  B01D2323/42 ,  C12M25/12

摘要： The present invention relates to a hollow microfiber comprising (1) one or more cell-adhesive layers having a cell-adhesive hydrogel, (2) an outer shell layer having a high-strength hydrogel that covers the outer periphery of the cell-adhesive layer that is positioned farthest from the center axis among the one or more cell-adhesive layers, and (3) a cell layer that covers the inner periphery of the cell-adhesive layer that is positioned closest to the center axis among the one or more cell-adhesive layers. The present invention also relates to a method of manufacturing the hollow microfiber and a kit for carrying out the manufacturing method.

摘要翻译： 本发明涉及一种中空微细纤维，其包含（1）一种或多种具有细胞粘合剂水凝胶的细胞粘合剂层，（2）具有覆盖细胞粘附层外周的高强度水凝胶的外壳层 在所述一个或多个电池 - 粘合剂层中位于距离所述中心轴线最远的电池层，以及（3）在所述一个或多个电池单元中覆盖所述电池 - 粘合剂层的位于最靠近所述中心轴线的内周的电池层 粘附层。 本发明还涉及制造中空微纤维的方法和用于实施该制造方法的试剂盒。

公开(公告)号：EP2623530B1

公开(公告)日：2015-03-04

申请号：EP11828738.2

申请日：2011-09-07

申请人： Terumo Kabushiki Kaisha ,  The University of Tokyo

发明人： HEO, Yun-Jung ,  SHIBATA, Hideaki ,  KAWANISHI, Tetsuro ,  TAKEUCHI, Shoji ,  MATSUNAGA, Yukiko ,  OKITSU, Teru

IPC分类号： C08F290/06 ,  C08F220/36 ,  C08F220/54 ,  C08G65/333 ,  G01N21/78 ,  A61B5/00 ,  A61B5/145 ,  G01N21/77 ,  G01N21/64

CPC分类号： A61B5/0071 ,  A61B5/14532 ,  C08F220/36 ,  C08F220/54 ,  C08F290/062 ,  G01N21/6428 ,  G01N2021/7786 ,  Y10S436/80

公开(公告)号：EP2219032A1

公开(公告)日：2010-08-18

申请号：EP08854712.0

申请日：2008-11-26

申请人： The University of Tokyo

发明人： TAKEUCHI, Shoji ,  SUZUKI, Hiroaki ,  OTA, Sadao ,  TAN, Wei-Heong

IPC分类号： G01N33/543 ,  G01N33/68 ,  G01N37/00

CPC分类号： C40B60/00 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/041 ,  B01L2300/0864 ,  B01L2400/0487 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/92 ,  G01N2405/04

摘要： There is provided a planar lipid bilayer array formed by microfluidic technique and a method of analysis using the planar lipid bilayers, providing the advantages such as portability, decreased analysis time, a smaller amount of required reagents, and parallel automation with high reproducibility. The planar lipid bilayer array formed by microfluidic technique is a planar lipid bilayer array formed by microfluidic technique (PDMS device) 1 saturated with water by preliminarily immersing in water, comprising microchannels 2 connected to an inlet of a microfluidic channel and arranged in parallel, and microchambers 3 having apertures on both sides of the microchannel 2.

摘要翻译： 提供了一种通过微流体技术和使用该平面脂双层分析的方法形成的平面脂双层阵列，从而提供的优点是：如便携性，减少分析时间，所需的试剂的量较小，并且平行自动化以高再现性。 通过微流体技术形成的平面脂双层阵列是通过微流体技术形成的平面脂双层阵列（PDMS装置）1通过在水中预先浸泡，包括在一个微流体通道的入口连接到和平行地配置微通道2水饱和，并 微室的微通道的bothsides具有光圈3 2

公开(公告)号：EP2216647A1

公开(公告)日：2010-08-11

申请号：EP08854654.4

申请日：2008-11-26

申请人： The University of Tokyo

发明人： TAKEUCHI, Shoji ,  KURIBAYASHI, Kaori

IPC分类号： G01N27/333 ,  B01J13/04 ,  G01N27/416 ,  G01N33/544

CPC分类号： G01N33/48728 ,  A61K9/1277

摘要： There is provided a method of preparing organic-solvent-free artificial lipid membranes formed over microapertures of a substrate and a substrate for holding the artificial lipid membranes. The method of preparing artificial lipid membranes over microapertures of a substrate includes forming a lipid pattern 5 on the perimeter of microapertures 4 formed in a substrate 1, swelling the lipid pattern 5 by an electroformation method, fusing the swelled lipid pattern, and further swelling the fused lipid pattern for each microaperture to prepare dome-shaped organic-solvent-free lipid membranes 7.

摘要翻译： 提供了一种制备在基片形成microapertures和基板无有机溶剂的人工脂质膜，用于保持人工脂质膜的方法。 在基板的microapertures制备人工脂质膜的方法包括：在一个基片1而形成microapertures 4的周边形成的脂质图案5，通过在电形成方法肿胀脂质图案5，熔融的溶胀脂质图案，并且进一步溶胀 对于每个microaperture制备圆顶状有机溶剂无脂膜融合脂质图案7