公开(公告)号：EP2238261A1

公开(公告)日：2010-10-13

申请号：EP08870439.0

申请日：2008-12-22

申请人： Verenium Corporation

发明人： WEINER, David ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin ,  DE SOUZA, Mervyn, L. ,  KOLLMANN, Sherry

IPC分类号： C12Q1/533 ,  C12N9/90 ,  C12N15/61 ,  A61K38/52 ,  C07K16/40

CPC分类号： C12N9/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A23G3/366 ,  A23G4/123 ,  A23G4/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  A61K38/52 ,  C12Q1/533 ,  C12Y501/00 ,  C12Y501/01

摘要： This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re¬ arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids. Thus, the invention provides enzymes, compositions, methods for production of pharmaceutical compositions, pharmaceutical intermediates, antibiotics, sweeteners, peptide enzymes, peptide hormones, fuel and fuel additive compositions, foods and food additives, beverage and beverage additives, feeds and feed additives, drugs and drug additives, dietary supplements, textiles, wood, paper, pulp, and detergents comprising the polypeptides or polynucleotides in accordance with the invention.

公开(公告)号：EP2238242A1

公开(公告)日：2010-10-13

申请号：EP08869698.4

申请日：2008-12-31

申请人： Verenium Corporation

发明人： WEINER, David ,  LUGINBUHL, Peter ,  BUENO, Analia ,  CUENCA, Joslin ,  MARASCO, Erin

IPC分类号： C12N9/10 ,  C12N9/02 ,  C12Q1/48 ,  C12Q1/26 ,  C07K14/38 ,  C07K16/40 ,  C07K9/00 ,  A61K38/44 ,  A61K38/45

CPC分类号： C12N9/1096 ,  A21D8/042 ,  A61K38/00 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0014 ,  C12N9/10 ,  C12N15/1137 ,  C12N15/63 ,  C12N15/86 ,  C12P7/10 ,  C12Q1/6876 ,  C12Y206/01021 ,  D06L4/40 ,  D06M16/003 ,  G01N33/573 ,  Y02E50/16 ,  Y02P20/52 ,  Y02T50/678

摘要： This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., damino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine + 2-oxoglutarate pyruvate + D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid + H 2 O + acceptor a 2-oxo acid + NH 3 + reduced acceptor. Thus, the invention provides enzymes, compositions, methods for production of pharmaceutical compositions, pharmaceutical intermediates, antibiotics, sweeteners, peptide enzymes, peptide hormones, fuel and fuel additive compositions, foods and food additives, beverage and beverage additives, feeds and feed additives, drugs and drug additives, dietary supplements, textiles, wood, paper, pulp, and detergents comprising the polypeptides or polynucleotides in accordance with the invention.

公开(公告)号：EP1999256A2

公开(公告)日：2008-12-10

申请号：EP07758102.3

申请日：2007-03-07

申请人： Verenium Corporation

发明人： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC分类号： C12N9/10

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.