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公开(公告)号：EP0402094A3
公开(公告)日：1991-05-22
申请号：EP90306092.9
申请日：1990-06-05
CPC分类号： C12Q1/28
摘要： A substrate or an enzymatic activity in a body fluid can be measured accurately without influences of interfering substances such as bilirubin by reacting an oxidase corresponding to an analyte with the analyte or an oxidase corresponding to a substance produced by enzymatic reaction with the substance in a measuring system containing one or more cationic and/or amphoteric surfactants, followed by optical measurement of hydrogen peroxide produced by the reaction.
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公开(公告)号：EP0127179B1
公开(公告)日：1987-11-25
申请号：EP84106143.5
申请日：1984-05-29
IPC分类号： C07D257/04 , C12Q1/26
CPC分类号： C07D257/04 , C09B50/02 , C12Q1/26
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14.发明公开Process for quantitative determination of substrate treated with oxidase 失效维他命Zur定量Bestimmung eines mit氧化酶behandelten基材。
公开(公告)号：EP0100217A2
公开(公告)日：1984-02-08
申请号：EP83304262.5
申请日：1983-07-22
发明人： Sakata, Yoshitsugu , Miyashita, Yoshinobu , Hamanaka, Tadashi , Kodera, Hiroyuki , Miki, Yutaka , Yamanishi, Kazuhiko , Hanada, Toshiro
IPC分类号： C12Q1/26
摘要： A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent. Further, the peroxidase can be replaced by (b') iron complex of porphyrin or ion chelate of complexane.
摘要翻译： 样品中的底物可以通过测量通过用对应于待测定底物的特定氧化酶处理样品而产生的比色超氧化物离子来测定，所述氧化酶处理和所测得的超氧化物离子是通过使用包含（ a）氧化酶，（b）过氧化物酶，（c）苯酚和/或胺，（d）硫醇化合物，（e）待还原的着色剂，以及必要时（f）螯合剂。 此外，过氧化物酶可以由（b min）卟啉的铁络合物或络合物的离子螯合物代替。
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公开(公告)号：EP1182213A1
公开(公告)日：2002-02-27
申请号：EP01113996.1
申请日：2001-06-08
CPC分类号： C12N9/0006 , C07K14/4737 , C07K2319/00 , C12N9/16 , C12N9/2471 , C12Y101/01049 , C12Y302/01023
摘要： Disclosed is a hybrid enzyme containing a foreign peptide, the hybrid enzyme having an enzyme activity similar to that prior to the substitution or insertion of the peptide, and having a property that the hybrid enzyme activity is modulated or modified when a material having binding ability to the peptide introduced by substitution or insertion is bound to the peptide moiety. Using the hybrid enzyme, it becomes possible to assay a trace amount of CRP in a sample by a homogeneous colorimetry, and a macromolecule materials can be easily assayed in a homogeneous system.
摘要翻译： 本发明公开了含有外来肽的杂合酶，所述杂合酶具有与肽取代或插入前相似的酶活性，具有如下特性：当具有结合能力的物质 通过取代或插入引入的肽与肽部分结合。 使用混合酶，可以通过均匀比色法测定样品中痕量的CRP，并且可以容易地在均相系统中测定大分子材料。
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16.发明授权Method for measuring the amount of constituent contained in specific lipotrotein 失效用于测量包含在特定脂蛋白的组分的量的方法
公开(公告)号：EP0754948B1
公开(公告)日：2001-10-17
申请号：EP96111431.1
申请日：1996-07-16
发明人： Miki, Yutaka , Hanada, Toshiro , Tanaka, Kiyoko
IPC分类号： G01N33/68 , G01N33/92 , G01N33/53 , G01N33/487 , G01N21/59
CPC分类号： G01N33/92 , G01N21/59 , G01N2800/044 , Y10S435/805 , Y10S435/962 , Y10S436/805 , Y10S436/81 , Y10S436/811 , Y10S436/824 , Y10S436/825
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17.发明公开
公开(公告)号：EP0821239A2
公开(公告)日：1998-01-28
申请号：EP97112537.2
申请日：1997-07-22
发明人： Miki, Yutaka , Imajo, Nobuko , Hanada, Toshiro
CPC分类号： G01N33/92 , G01N2800/044 , Y10S435/975
摘要： The present invention is to provide a method for measuring an amount of cholesterol in low density lipoproteins (LDL-cholesterol) in a sample specifically at high accuracy and a reagent used in this method, and the present invention can attain such effect that direct measuring an amount of LDL-cholesterol by widely used automatic analyzers can be conducted by using the invention, which has not been possible after known methods.
摘要翻译： 本发明提供一种以高精度测定样品中的低密度脂蛋白（LDL-胆固醇）的胆固醇量的方法和该方法中使用的试剂，本发明可以获得直接测定 通过广泛使用的自动分析仪的LDL-胆固醇的量可以通过使用本发明进行，这在现有方法之后是不可能的。
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公开(公告)号：EP0124909B1
公开(公告)日：1989-02-15
申请号：EP84105266.5
申请日：1984-05-09
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公开(公告)号：EP0100217B1
公开(公告)日：1987-10-07
申请号：EP83304262.5
申请日：1983-07-22
发明人： Sakata, Yoshitsugu , Miyashita, Yoshinobu , Hamanaka, Tadashi , Kodera, Hiroyuki , Miki, Yutaka , Yamanishi, Kazuhiko , Hanada, Toshiro
IPC分类号： C12Q1/26
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公开(公告)号：EP0223977A1
公开(公告)日：1987-06-03
申请号：EP86113651.3
申请日：1986-10-02
IPC分类号： C12Q1/28 , C12Q1/26 , G01N33/535
CPC分类号： G01N33/548 , C12Q1/28
摘要： A process for measuring the degree of coloring of a color producing reagent to be reduced such as a tetrazolium salt with color developing assistants can be applied to clinical examinations such as determination of the amount of H 2 O 2 and determination of peroxidase activity without much suffering from influences of ascorbic acid, bililubin, etc. present in samples.
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