公开(公告)号：EP3344760A1

公开(公告)日：2018-07-11

申请号：EP16843195.5

申请日：2016-09-06

申请人： The UAB Research Foundation ,  Emory University ,  Children's Healthcare Of Atlanta, Inc.

发明人： LAMB, Lawrence S. ,  SPENCER, H. Trent ,  GILLESPIE, G. Yancey

IPC分类号： C12N5/22 ,  C12N5/0783 ,  C07K14/705 ,  A61K35/17 ,  A61K31/495 ,  A61K39/00

CPC分类号： A61K35/17 ,  A61K31/495 ,  A61K39/0011 ,  A61K2039/5156 ,  A61K2039/5158 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/7155 ,  C07K16/30 ,  C07K2319/70 ,  C12N5/0636 ,  C12N5/0638 ,  C12N9/12 ,  C12N2501/998 ,  C12N2510/00 ,  C12Y207/10001

摘要： The present disclosure provides novel cell compositions engineered to express at least a chimeric antigen receptor and a survival factor. Methods of using such cell compositions are also described.

公开(公告)号：EP3325018A1

公开(公告)日：2018-05-30

申请号：EP16828669.8

申请日：2016-07-22

申请人： Duke University

发明人： GERSBACH, Charles, A. ,  CRAWFORD, Gregory, E. ,  REDDY, Timothy, E. ,  KLANN, Tyler, S.

IPC分类号： A61K48/00 ,  C07K19/00 ,  C12N5/22 ,  C12N15/63 ,  C12N15/65 ,  C12N15/867

CPC分类号： C12N15/1079 ,  C07K14/4703 ,  C07K14/4705 ,  C07K2319/00 ,  C12N9/22 ,  C12N2740/16043 ,  C12Y301/00 ,  C12Q2525/301 ,  C12Q2563/107

摘要： Disclosed herein are methods of using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) 9-based epigenomic editing systems for high-throughput screening of regulatory element function.

公开(公告)号：EP3272859A1

公开(公告)日：2018-01-24

申请号：EP16765134.8

申请日：2016-03-18

申请人： Kyoto University

发明人： GOTOH, Shimpei ,  YAMAMOTO, Yuki ,  KONISHI, Satoshi ,  MISHIMA, Michiaki

IPC分类号： C12N5/22 ,  C12N5/0735

摘要： This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3β inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.

摘要翻译： 本发明提供了一种从多能干细胞稳定产生气道上皮细胞的方法。 具体而言，本发明涉及由多能干细胞制造气道上皮细胞的方法，其包括以下步骤：（1）在含有激活素A和GSK3β抑制剂的培养基中培养多能干细胞; （2）在含有BMP抑制剂和TGFβ抑制剂的培养基中培养步骤（1）中获得的细胞; （3）在含有BMP4，视黄酸和GSK3β抑制剂的培养基中培养步骤（2）中获得的细胞; （5）将步骤（3）后获得的细胞在含有GSK3β抑制剂，FGF10和ROCK抑制剂的培养基中进行三维培养; （6）使步骤（5）中获得的近端气道上皮祖细胞在含有ROCK抑制剂的培养基中进行三维培养。

公开(公告)号：EP3122872A4

公开(公告)日：2017-08-30

申请号：EP15768776

申请日：2015-03-26

申请人： THE SALK INST FOR BIOLOGICAL STUDIES

发明人： EVANS RONALD M ,  YOSHIHARA EIJI ,  DOWNES MICHAEL R ,  YU RUTH T ,  ATKINS ANNETTE R

IPC分类号： C12N5/10 ,  A61K35/39 ,  C12N5/074 ,  C12N5/22

CPC分类号： C12N15/85 ,  A61K35/12 ,  A61K35/35 ,  A61K35/39 ,  A61K35/51 ,  A61K35/545 ,  A61K38/00 ,  A61K48/00 ,  C07K14/70567 ,  C07K14/721 ,  C12N5/0676 ,  C12N15/86 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2710/10032 ,  C12N2710/10043

摘要： The invention features compositions comprising in vitro generated beta cells capable of glucose-stimulated insulin secretion, methods of inducing beta cell maturation from embryonic or induced pluripotent stem cell-derived beta-like cells, and methods of using in vitro generated beta cells for the treatment of type 1 diabetes, type 2 diabetes, or a related disorder.

摘要翻译： 本发明的特征在于包含能够葡萄糖刺激的胰岛素分泌的体外产生的β细胞的组合物，从胚胎或诱导的多能干细胞衍生的β样细胞诱导β细胞成熟的方法，以及使用体外产生的β细胞进行治疗的方法 1型糖尿病，2型糖尿病或相关疾病。

公开(公告)号：EP2962113A4

公开(公告)日：2016-11-09

申请号：EP14756627

申请日：2014-02-28

申请人： BIOCARE MEDICAL LLC

发明人： TACHA DAVID ,  QI WEIMIN

IPC分类号： G01N33/574 ,  C07K16/28 ,  C07K16/32 ,  C12N5/16 ,  C12N5/22

CPC分类号： C07K16/18 ,  C07K16/28 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/565 ,  G01N33/5017 ,  G01N33/574 ,  G01N33/57423 ,  G01N33/57484 ,  G01N33/57488 ,  G01N33/577

摘要： The present invention is related to the anti-p40 antibodies, kits, cocktails, and use of anti-p40 antibodies for detection of cancer.

公开(公告)号：EP2484763B1

公开(公告)日：2015-10-21

申请号：EP12154752.5

申请日：2007-08-21

申请人： Zensun (Shanghai) Science and Technology Limited

发明人： Zhou, Mingdong, Dr.

IPC分类号： C12N15/11 ,  C12N15/12 ,  C12N15/52 ,  C12N15/63 ,  C12N15/85 ,  C12N15/86 ,  C12N9/48 ,  C12N5/16 ,  C12N5/22 ,  C07H21/00 ,  C07K16/16 ,  A61K38/45 ,  C07K16/40 ,  C12Q1/48 ,  G01N33/68 ,  C12N9/12

CPC分类号： C07K16/40 ,  A61K38/45 ,  C12N9/1205 ,  C12Q1/485 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/4756

公开(公告)号：EP2730654A2

公开(公告)日：2014-05-14

申请号：EP13184566.1

申请日：2007-08-21

申请人： Zensun (Shanghai) Science and Technology Limited

发明人： Zhou, Mingdong

IPC分类号： C12N15/11 ,  C12N15/12 ,  C12N15/52 ,  C12N15/63 ,  C12N15/85 ,  C12N15/86 ,  C12N9/48 ,  C12N5/16 ,  C12N5/22 ,  C07H21/00 ,  C07K16/16 ,  A61K38/45 ,  C07K16/40 ,  C12Q1/48 ,  G01N33/68 ,  C12N9/12

CPC分类号： C07K16/40 ,  A61K38/45 ,  C12N9/1205 ,  C12Q1/485 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/4756

摘要： The present invention relates to an isolated polypeptide comprising an amino acid sequence having at least 70% identity to the amino acid sequence of SEQ ID NO:2 or to the amino acid sequence of SEQ ID NO:25; or encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or the amino acid sequence of SEQ ID NO: 25, wherein the polypeptide is capable of phosphorylating myosin light chain. The present invention also relates to an isolated nucleic acid encoding said polypeptide; a composition and a kit comprising the polypeptide or the nucleic acid; a vector comprising the isolated nucleic acid; an isolated host cell comprising the nucleic acid or the vector; an antibody that specifically binds to the polypeptide of the present invention; and a transgenic non-human animal which expresses the nucleic acid of the present invention. The invention further relates to a method of detecting the presence of a nucleic acid of the present invention; and a method of producing a neukinase polypeptide of the present invention.

摘要翻译： 本发明涉及分离的多肽，其包含与SEQ ID NO：2的氨基酸序列或SEQ ID NO：25的氨基酸序列具有至少70％同一性的氨基酸序列; 或编码包含SEQ ID NO：2的氨基酸序列或SEQ ID NO：25的氨基酸序列的多肽，其中所述多肽能够磷酸化肌球蛋白轻链。 本发明还涉及编码所述多肽的分离的核酸; 包含所述多肽或所述核酸的组合物和试剂盒; 包含分离的核酸的载体; 包含核酸或载体的分离的宿主细胞; 特异性结合本发明多肽的抗体; 和表达本发明核酸的转基因非人动物。 本发明进一步涉及检测本发明核酸存在的方法; 以及生产本发明的新生素酶多肽的方法。

公开(公告)号：EP2046834B9

公开(公告)日：2013-04-10

申请号：EP07792618.6

申请日：2007-08-10

申请人： ONO PHARMACEUTICAL CO., LTD. ,  Medarex, Inc.

发明人： POGUE, Sarah, L. ,  KORMAN, Alan J. ,  CARDARELLI, Josephine, M. ,  SRINIVASAN, Mohan ,  CHEN, Bingliang ,  BELL, Alasdair, F.

IPC分类号： C07K16/24 ,  A61K39/395 ,  A61P3/10 ,  A61P9/10 ,  A61P29/00 ,  A61P35/00 ,  A61P37/00 ,  A61P37/06 ,  A61P43/00 ,  C12P21/08 ,  C12N5/22 ,  C12N15/13

CPC分类号： C07K16/24 ,  A61K2039/505 ,  C07K2299/00 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92

公开(公告)号：EP1068296B1

公开(公告)日：2011-08-10

申请号：EP99916193.8

申请日：1999-03-30

申请人： GERON CORPORATION

发明人： GAETA, Federico, C., A.

IPC分类号： C12N5/22 ,  C12N9/00

CPC分类号： C12N9/1241 ,  A61K39/0011 ,  A61K2039/5154 ,  A61K2039/5156 ,  A61K2039/5158 ,  A61K2039/55522 ,  C12N9/1276

摘要： The invention provides a method of activating a T lymphocyte by contacting the T lymphocyte with a dendritic cell (DC) that presents a telomerase reverse transcriptase (TRT) peptide in the context of a MHC class I or MHC class II molecule. The DC may be pulsed with a TRT polypeptide or may comprise a recombinant polynucleotide encoding a TRT such as hTRT. The invention also provides DCs comprising a recombinant TRT polynucleotide. The methods and compositions of the invention are used in prevention and treatment of cancers and other cell proliferation diseases or conditions.

公开(公告)号：EP2316856A1

公开(公告)日：2011-05-04

申请号：EP10013085.5

申请日：2003-10-17

申请人： Genmab A/S

发明人： Teeling, Jessica ,  Ruuls, Sigrid ,  Glennie, Martin ,  Van De Winkel, Jan G. J. ,  Parren, Paul ,  Petersen, Jorgen ,  Baadsgaard, Ole D. M. Sc. ,  Huang, Haichun

IPC分类号： C07K16/28 ,  C07K16/30 ,  C12N5/22 ,  C12N5/24 ,  C12N15/63

CPC分类号： C07K16/2887 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/4258 ,  C07K2317/21 ,  C07K2317/54 ,  C07K2317/56 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  G01N33/686

摘要： Isolated human monoclonal antibodies which bind to and inhibit human CD20, and related antibody-based compositions and molecules, are disclosed. The human antibodies can be produced by a transfectoma or in a non-human transgenic animal, e.g ., a transgenic mouse, capable of producing multiple isotypes of human monoclonal antibodies by undergoing V-D-J recombination and isotype switching. Also disclosed are pharmaceutical compositions comprising the human antibodies, non-human transgenic animals and hybridomas which produce the human antibodies, and therapeutic and diagnostic methods for using the human antibodies.

摘要翻译： 公开了结合并抑制人CD20的分离的人单克隆抗体以及相关的基于抗体的组合物和分子。 人抗体可以由转染瘤或非人转基因动物例如转基因小鼠产生，转基因小鼠能够通过进行V-D-J重组和同种型转换来产生人单克隆抗体的多重同种型。 还公开了包含人抗体，非人转基因动物和产生人抗体的杂交瘤的药物组合物，以及使用人抗体的治疗和诊断方法。