利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

29 条记录 (耗时 0.027 秒)

    PHASE-PROTECTING REAGENT FLOW ORDERINGS FOR USE IN SEQUENCING-BY-SYNTHESIS
    23.
    发明公开
    PHASE-PROTECTING REAGENT FLOW ORDERINGS FOR USE IN SEQUENCING-BY-SYNTHESIS 审中-公开

    公开(公告)号：EP3366782A1

    公开(公告)日：2018-08-29

    申请号：EP18151358.1

    申请日：2012-04-05

    申请人： Life Technologies Corporation

    发明人： HUBBELL, Earl ,  SCHULTZ, Jonathan

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607

    摘要： A method for nucleic acid sequencing includes disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands with the sequencing primer and a polymerase operably bound therewith to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.

    SYSTEM AND METHOD INCORPORATING SOLID BUFFER
    24.
    发明公开
    SYSTEM AND METHOD INCORPORATING SOLID BUFFER 审中-公开
    包含固体缓冲器的系统和方法

    公开(公告)号：EP3158342A1

    公开(公告)日：2017-04-26

    申请号：EP15738170.8

    申请日：2015-06-16

    申请人： Life Technologies Corporation

    发明人： BALL, James ,  SCHULTZ, Jonathan

    IPC分类号： G01N33/84 ,  B01J8/00 ,  C12Q1/68

    摘要： A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

    摘要翻译： 缓冲悬浮液包括表面活性剂和固体缓冲剂颗粒，其零点电荷至少为1.2个pH单位，不同于缓冲悬浮液的pH。 缓冲悬浮液可通过将储备溶液与固体缓冲剂颗粒混合并滴定来制备。 预先形成pH敏感过程的方法包括从贮存器抽取缓冲悬浮液，从缓冲悬浮液中过滤固体缓冲剂颗粒，并将过滤后溶液施加到传感器。

    SEQUENCING DEVICE
    25.
    发明公开
    SEQUENCING DEVICE 审中-公开
    测序装置

    公开(公告)号：EP3157678A1

    公开(公告)日：2017-04-26

    申请号：EP15733595.1

    申请日：2015-06-17

    申请人： Life Technologies Corporation

    发明人： SCHULTZ, Jonathan ,  ROSWECH, Todd ,  HOSHIZAKI, Jon ,  CARRILLO, Albert ,  BALL, James

    IPC分类号： B01L3/00 ,  G01N35/10

    CPC分类号： C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

    摘要： A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    摘要翻译： 制备试剂的方法包括将药筒插入器械中。 盒包括多个试剂外壳，所述试剂外壳设置在盒的空腔中并且将端口暴露于盒的外部。 每个试剂外壳包括试剂容器，该试剂容器包括试剂和限定可压缩容积的内腔，开口通过试剂容器限定到内腔。 该方法还包括将多个流体端口连接到多个试剂外壳的开口; 通过所述流体端口施加溶液以至少部分地填充所述多个试剂外壳; 并且循环腔的压力，由此对于每个试剂腔，在增加压力期间，溶液进入试剂容器的内腔，与试剂结合并压缩可压缩容积，并且在减压过程中，可压缩容积 减少并且试剂通过开口喷射。