公开(公告)号：EP0149853A3

公开(公告)日：1986-09-17

申请号：EP84116437

申请日：1984-12-28

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.

发明人： Yamanishi, Kazuhiko ,  Hanada, Toshiro

IPC分类号： C12Q01/32

CPC分类号： C12Q1/32

摘要： In a process for measuring the activity of a dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazorium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used, deposition and staining of a formazan compound produced can be prevented and improvement in color-producing stability and enhancement of coloring can be attained.

公开(公告)号：EP0124909A3

公开(公告)日：1985-09-18

申请号：EP84105266

申请日：1984-05-09

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.

发明人： Yamanishi, Kazuhiko ,  Hanada, Toshiro

IPC分类号： C12Q01/32 ,  C12Q01/28 ,  C12Q01/60 ,  C12Q01/00

CPC分类号： C12Q1/28 ,  C12Q1/008 ,  C12Q1/60

摘要： A reduced form coenzyme can be determined by acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide and measuring the amount of hydrogen peroxide colorimetrically.

公开(公告)号：EP0100217A3

公开(公告)日：1985-04-10

申请号：EP83304262

申请日：1983-07-22

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.

发明人： Sakata, Yoshitsugu ,  Miyashita, Yoshinobu ,  Hamanaka, Tadashi ,  Kodera, Hiroyuki ,  Miki, Yutaka ,  Yamanishi, Kazuhiko ,  Hanada, Toshiro

IPC分类号： C12Q01/26 ,  G01N33/52

CPC分类号： C12Q1/62 ,  C12Q1/26 ,  C12Q1/54 ,  C12Q1/60

摘要： A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent. Further, the peroxidase can be replaced by (b') iron complex of porphyrin or ion chelate of complexane.

公开(公告)号：EP0124909A2

公开(公告)日：1984-11-14

申请号：EP84105266.5

申请日：1984-05-09

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.

发明人： Yamanishi, Kazuhiko ,  Hanada, Toshiro

IPC分类号： C12Q1/32 ,  C12Q1/28 ,  C12Q1/60 ,  C12Q1/00

CPC分类号： C12Q1/28 ,  C12Q1/008 ,  C12Q1/60

摘要： A reduced form coenzyme can be determined by acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide and measuring the amount of hydrogen peroxide colorimetrically.