公开(公告)号：EP2839035A1

公开(公告)日：2015-02-25

申请号：EP13778720.6

申请日：2013-04-16

申请人： Biological Dynamics, Inc.

发明人： SWANSON, Paul ,  TURNER, Robert ,  YANG, Kai ,  DOBROVOLSKAYA, Irina ,  LIU, David ,  KRISHNAN, Rajaram ,  CHARLOT, David ,  TU, Eugene ,  McCANNA, James ,  KUMOSA, Lucas

IPC分类号： C12Q1/68 ,  C12Q1/00

CPC分类号： B01L3/502715 ,  B01D57/02 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6806 ,  G01N27/447 ,  G01N27/44704 ,  G01S5/163 ,  G01S11/12 ,  G06F3/0346

摘要： The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.

摘要翻译： 本发明包括用于从包含细胞的流体分离核酸的方法，装置和系统。 在各个方面，所述方法，装置和系统可允许需要最少量材料和/或导致从复杂流体例如血液或环境样品分离的高纯度核酸的快速程序。

公开(公告)号：EP2836813A1

公开(公告)日：2015-02-18

申请号：EP13776001.3

申请日：2013-04-05

申请人： APDN (B.V.I.) Inc.

发明人： BERRADA, Abdelkrim ,  LIANG, MingHwa, Benjamin ,  HAYWARD, James, Arthur ,  JUNG, Lawrence

IPC分类号： G01N1/28 ,  H05H1/00

CPC分类号： C12N15/101 ,  B01J19/08 ,  B01J19/088 ,  B01J2219/0879 ,  B01J2219/0894 ,  B01J2219/24 ,  C12N15/1006 ,  C12Q1/6806 ,  G01N27/447 ,  G01N27/44791

摘要： The invention provides a composition including DNA bonded to a plasma-treated surface, the plasma can be any suitable plasma, such as an argon plasma, a compressed air plasma, a flame-based plasma or a vacuum plasma. Surfaces treatable by the methods of the invention include ceramic, metal, fabric and organic polymer surfaces. The DNA can be any DNA, such as a marker DNA, which can be linear or circular, single-stranded or double stranded and from about 25 bases to about 10,000 bases in length. Also provided is a method of binding DNA to a surface, including the steps of exposing the surface to a plasma to produce a plasma-treated surface; and applying DNA to the plasma-treated surface to produce surface bound DNA on the treated surface. A system for binding DNA to a surface is also disclosed, the system includes a plasma generator adapted to treating a surface with a plasma to produce a plasma-treated surface; and an applicator containing DNA adapted to applying DNA to the plasma-treated surface to produce surface bound DNA on the plasma-treated surface.

摘要翻译： 本发明提供了包含与等离子体处理的表面结合的DNA的组合物，等离子体可以是任何合适的等离子体，诸如氩等离子体，压缩空气等离子体，火焰基等离子体或真空等离子体。 可用本发明的方法处理的表面包括陶瓷，金属，织物和有机聚合物表面。 DNA可以是任何DNA，例如标记DNA，其可以是线性或环状的，单链或双链的，并且长度为约25至约10,000个碱基。 还提供了将DNA结合到表面的方法，包括将表面暴露于等离子体以产生经等离子体处理的表面的步骤; 并将DNA应用于等离子体处理的表面以在处理过的表面上产生表面结合的DNA。 还公开了用于将DNA结合到表面的系统，该系统包括等离子体发生器，其适于用等离子体处理表面以产生等离子体处理过的表面; 和含有DNA的涂药器，该DNA适于将DNA应用于等离子体处理的表面以在等离子体处理的表面上产生表面结合的DNA。

公开(公告)号：EP2825646A1

公开(公告)日：2015-01-21

申请号：EP13717812.5

申请日：2013-03-15

申请人： Cambridge Enterprise Limited

发明人： CHUA, Yii Leng

IPC分类号： C12N15/10

CPC分类号： B01D15/20 ,  B01D11/02 ,  B01D11/0219 ,  B01D15/00 ,  B01D15/02 ,  B01D15/24 ,  B01D15/245 ,  B01D15/26 ,  B01D17/02 ,  B01D17/0202 ,  C12N15/1006 ,  C12N15/101 ,  C12N15/1017

摘要： A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.

摘要翻译： 描述了从多孔固相中获得液体的方法。 该方法包括在多孔固相的第一端形成液体密封，液体与液体结合在一起，其中液体密封液体与与固相结合的液体不混溶，并且在多孔固相上施加压力差 以使不相容的液体朝向多孔固相的第二端移动通过多孔固相，从而将结合到多孔固相的液体朝向第二端移动，并从第二端释放该液体。 与没有形成液体密封的相应方法相比，使用这种方法从固相中回收液体。 在优选的实施方案中，用于形成液体密封的液体是矿物油。 该方法在核酸提取中具有特异性应用，利用核酸捕获到固相。 还描述了用于执行方法的套件和装置。

公开(公告)号：EP2757156A1

公开(公告)日：2014-07-23

申请号：EP12832581.8

申请日：2012-08-28

申请人： Sony Corporation

发明人： NAKAMURA, Tomohiko ,  SAKAMOTO, Naohisa ,  YOTORIYAMA, Tasuku ,  ITO, Kazumine

IPC分类号： C12N15/09 ,  B01D15/04 ,  B01J39/04 ,  B01J41/04 ,  C12M1/00 ,  C12Q1/68 ,  G01N30/88

CPC分类号： C12N15/101 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01J39/04 ,  B01J41/04 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6806 ,  G01N30/88 ,  G01N30/96 ,  G01N2030/8827 ,  C12Q2523/308 ,  B01J39/043 ,  B01J41/043 ,  B01J39/05 ,  B01J41/05

摘要： [Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency.
[Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used.

摘要翻译： 提供一种纯化核酸的方法，其中操作简单，并且可以在短时间内以高效率提取核酸。 [解决方案]一种纯化核酸的方法，包括在含有正离子交换树脂和负离子交换树脂的离子交换树脂10中将含有核酸的样品中的物质吸附的步骤。 作为正离子交换树脂，可以使用排阻极限分子量低于第一正离子交换树脂的第一正离子交换树脂和第二正离子交换树脂。

公开(公告)号：EP2737088A1

公开(公告)日：2014-06-04

申请号：EP12819596.3

申请日：2012-07-27

申请人： Biogen Idec MA Inc.

发明人： RAY, Soma

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00

CPC分类号： C12N15/101 ,  C07H1/06 ,  C07H21/00 ,  C12N15/1003 ,  C12N2710/22011 ,  C12Q1/701

摘要： In one aspect, the disclosure provides methods for isolating nucleic acid from a Cerebrospinal Fluid (CSF) sample. In one aspect, the disclosure provides methods for determining the amount of JC virus DNA in a sample.

公开(公告)号：EP2655617A1

公开(公告)日：2013-10-30

申请号：EP11804700.0

申请日：2011-12-23

申请人： Qiagen GmbH

发明人： SCHLUMPBERGER, Martin

IPC分类号： C12N15/10 ,  G01N1/36

CPC分类号： G01N1/30 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/101

摘要： The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample.

摘要翻译： 本发明涉及一种用于处理嵌蜡生物样品的方法，使用聚（有机硅氧烷）来液化嵌入蜡的生物样品的包埋介质和用于处理蜡嵌入的生物样品的试剂盒。

公开(公告)号：EP2576779A1

公开(公告)日：2013-04-10

申请号：EP11726377.2

申请日：2011-06-01

申请人： Qiagen GmbH

发明人： FABIS, Roland ,  PETZEL, Jan

IPC分类号： C12N15/10

CPC分类号： C12N15/1013 ,  C12N15/101

摘要： The present invention relates to a method for isolating and/or purifying one or more nucleic acid(s) from a sample, comprising the steps of essentially separating the nucleic acid(s) from the sample by binding the nucleic acid(s) to a solid phase by means of a non-chaotropic water-soluble binding ligand at a first pH (pH I), and essentially eluting the nucleicacid(s) from the solid phase at a second pH (pH II). The invention further relates to a kit for isolating and/or purifying nucleic acid(s) from a sample and/or for protecting nucleic acid(s).

摘要翻译： 本发明涉及用于从样品中分离和/或纯化一个或多个核酸的方法，其包括以下步骤：通过将核酸与一种或多种核酸结合至基本上将基因与样品分离， 固相，其在第一pH（pH I）下通过非离液序列的水溶性结合配体，并且在第二pH（pH II）下基本上从固相中洗脱核酸。 本发明还涉及用于从样品中分离和/或纯化核酸和/或保护核酸的试剂盒。

公开(公告)号：EP2574670A1

公开(公告)日：2013-04-03

申请号：EP11865705.5

申请日：2011-12-27

申请人： Nanohelix Co., Ltd

发明人： KO, Minsu ,  KIM, Young Cheol ,  LEE, Siseok

IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N30/90

CPC分类号： C12N15/101 ,  C07H21/00 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2523/308 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2565/137 ,  G01N30/90 ,  G01N2030/8827 ,  Y10T436/255

摘要： Provided is a method for rapid nucleic acid purification, and the method for rapid nucleic acid isolation according to the present invention is very useful in diagnosing causes of disease or detecting a target gene; can be used in molecular diagnosis of causes of disease more rapidly and conveniently, as compared with the existing nucleic acid isolation method requiring complicated and special equipment; does not require skills therefor, thereby allowing an ordinary person to personally conduct isolation of nucleic acid for analyzing causes of disease and further solving the existing inconvenience caused by directly going to the hospitals or health clinical centers; and can analyze causes of disease more promptly.

摘要翻译： 提供了一种快速核酸纯化方法，根据本发明的快速核酸分离方法在诊断病因或检测靶基因方面非常有用; 与现有的需要复杂和特殊设备的核酸分离方法相比，可以更迅速方便地用于疾病原因的分子诊断; 不需要技能，从而允许普通人亲自进行核酸分离，分析疾病原因，进一步解决直接前往医院或健康临床中心造成的不便之处; 并能及时分析疾病原因。

公开(公告)号：EP1623039B1

公开(公告)日：2012-08-01

申请号：EP04752257.8

申请日：2004-05-13

申请人： Ibis Biosciences, Inc.

发明人： HOFSTADLER, Steven, A. ,  CUMMINS, Lendell, L.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/00

CPC分类号： C12N15/101 ,  C12Q1/6806 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2565/627

摘要： The present invention provides methods for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.

公开(公告)号：EP2394152A2

公开(公告)日：2011-12-14

申请号：EP10732498.0

申请日：2010-02-03

申请人： NetBio, Inc.

发明人： SELDEN, Richard, F. ,  TAN, Eugene

IPC分类号： G01N1/34 ,  C12N15/10 ,  B01L3/00

CPC分类号： B01L3/502753 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/50825 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/043 ,  B01L2400/0487 ,  C12N15/1003 ,  C12N15/101

摘要： A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.

摘要翻译： 用于从仪器使用的未处理样品装置分离核酸，细胞裂解物和细胞悬浮液的独立装置包括至少一个输入，和：（i）大流体组分，包括用于接收未处理样品的室 从收集装置和至少一个填充液体净化试剂储存容器; 和（ii）通过至少一个微流体元件与所述宏流体组分连通的微流体组分，所述微流体组分还包含至少一个核酸纯化基质; 和（iii）通过微流体元件和核酸纯化基质的至少一个界面端口连接到仪器上用于驱动所述液体纯化试剂的驱动机构的至少一个界面端口，其中仅对所述装置的输入通过所述室和所述界面端口 驱动机构。