公开(公告)号：EP2494033A1

公开(公告)日：2012-09-05

申请号：EP10827464.8

申请日：2010-10-28

Applicant: The University of North Carolina At Chapel Hill ,  Sapienza Universita di Roma

Inventor： REID, Lola, M. ,  WANG, Yunfang ,  CARDINALE, Vincenzo ,  GAUDIO, Eugenio ,  CARPINO, Guido ,  ALVARO, Domenico ,  CUI, Cai-Bin

IPC: C12N5/071

CPC classification number: C12N5/0672 ,  A61K35/413 ,  C12N5/0678 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2533/54 ,  C12N2533/80 ,  C12N2537/10

Abstract: The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands. Compositions comprising same, methods of identifying and isolating same, maintaining same in culture, expanding same in culture and differentiating or lineage restricting the same in vitro or in vivo to hepatic, biliary or pancreatic fates (e.g., as hepatocytes, cholangiocytes, and/or pancreatic islet cells) are also provided. Methods of using the multipotent cells and/or multipotent cell populations are also provided.

公开(公告)号：EP2403872A1

公开(公告)日：2012-01-11

申请号：EP10705891.9

申请日：2010-03-02

Applicant: Assistance Publique Hôpitaux De Paris

Inventor： POLAK, Michel ,  SCHARFMANN, Raphaël ,  ZERTAL-ZIDANI, Samia

IPC: C07K14/705 ,  C12N5/071

CPC classification number: C12N5/0676 ,  C07K14/705 ,  C12N5/0678 ,  C12N2501/999

Abstract: The present invention discloses a method for increasing the pool of Ngn3+ endocrine progenitor cells obtained from stem cells, by contacting said stem cells with a SUR1/Kir6.2 channel inhibitor. The invention also relates to a method for increasing the mass of pancreatic endocrine cells, in particular of β cells. The invention further concerns a method for treating diabetes.

公开(公告)号：EP1438418B1

公开(公告)日：2010-11-24

申请号：EP02768905.8

申请日：2002-09-26

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： HABENER, Joel, F. ,  ZULEWSKI, Henryk ,  THOMAS, Melissa, K. ,  ABRAHAM, Elizabeth, J. ,  VALLEJO, Mario ,  LEECH, Colin, A. ,  NOLAN, Anna, Louise ,  LECHNER, Andreas

IPC: C12Q1/00 ,  G01N33/53 ,  C12N5/02 ,  A61K48/00

CPC classification number: A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

Abstract: Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin and ABCG2 have been identified as molecular markers for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin and/or ABCG2-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

公开(公告)号：EP2233566A1

公开(公告)日：2010-09-29

申请号：EP09155331.3

申请日：2009-03-17

Applicant: Vrije Universiteit Brussel ,  OPUS NV

Inventor： Bouwens, Luc ,  Kunjom Mfopou, Josué

IPC: C12N5/077

CPC classification number: C12N5/0678 ,  A61K35/12 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/70 ,  C12N2506/02

Abstract: The invention relates to in vitro methods for producing pancreatic progenitor cells and cell populations from cells having definitive endoderm germ layer phenotype. The invention further encompasses so-obtained pancreatic progenitor cells and cell populations, compositions comprising the same, and further uses of said pancreatic progenitor cells and cell populations, such as inter alia for producing functional, insulin-producing beta cells. The invention is of particular relevance for medical applications, more specifically in the field of cell therapy of diabetes.

Abstract translation: 本发明涉及从具有确定的内胚层胚层表型的细胞产生胰腺祖细胞和细胞群的体外方法。 本发明还包括如此获得的胰腺祖细胞和细胞群，包含其的组合物，以及所述胰腺祖细胞和细胞群体的进一步用途，例如尤其用于产生功能性的产生胰岛素的β细胞。 本发明与医学应用特别相关，更具体地涉及糖尿病细胞治疗领域。

公开(公告)号：EP2087101A2

公开(公告)日：2009-08-12

申请号：EP07862249.5

申请日：2007-11-26

Applicant: Regents of the University of Minnesota

Inventor： VERFAILLIE, Catherine M. ,  CHASE, Lucas

IPC: C12N5/08

CPC classification number: C12N5/0678 ,  A01K2267/0325 ,  A61K35/12 ,  C12N2500/42 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2533/52

Abstract: The present invention relates to endodermal progenitor cells and methods of isolation, culture, differentiation and use thereof.

公开(公告)号：EP1391505B1

公开(公告)日：2009-01-28

申请号：EP02720586.3

申请日：2002-04-24

Applicant: Ajinomoto Co., Inc.

Inventor： TANIGUCHI, Hideki ,  SUZUKI, Atsushi

IPC: C12N5/08 ,  C12N15/09 ,  G01N33/50 ,  G01N33/15 ,  A61K35/39 ,  A61P1/18 ,  C12Q1/68 ,  C12Q1/02

CPC classification number: C12Q1/6881 ,  A61K35/12 ,  C12N5/0678 ,  C12N2503/02 ,  C12Q2600/158 ,  G01N33/5005

Abstract: A method of separating pancreatic stem cells from mammalian pancreas; a method of identifying mammalian pancreatic stem cells; and use of the pancreatic stem cells thus separated and identified. More specifically, a method of separating mammalian pancreatic stem cells and a method of identifying the same involving the step of analyzing the expression state of at least two marker proteins selected from the group consisting of c-Met, c-Kit, CD45, TER119 and Flk-1 or genes encoding the same.

公开(公告)号：EP1978090A1

公开(公告)日：2008-10-08

申请号：EP08002213.0

申请日：2001-08-09

Applicant: ES Cell International Pte Ltd.

Inventor： Lu, Kuanghui ,  Rubin, Lee ,  Pang, Kevin

IPC: C12N5/06 ,  C12N5/08

CPC classification number: C12N5/0647 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2500/02 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/125 ,  C12N2501/148 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2501/35 ,  C12N2501/39 ,  C12N2501/835 ,  C12N2510/02

Abstract: A method for obtaining differentiated pancreatic cell types comprising contacting a pancreatic progenitor cell with an adherent matrix and a differentiation medium comprising a cAMP elevating agent, at least 150 ng/ml PYY and at least 3% fetal bovine serum, wherein said progenitor cell gives rise to at least one differentiated pancreatic cell type, wherein the pancreatic progenitor cell is to be obtained from human embryonic tissue or a cell suspension of human embryonic tissue.

Abstract translation: 一种用于获得分化胰腺细胞类型的方法，包括使胰腺祖细胞与贴壁基质和包含cAMP升高剂，至少150ng / ml PYY和至少3％胎牛血清的分化培养基接触，其中所述祖细胞产生 至少一种分化胰腺细胞类型，其中所述胰腺祖细胞将从人胚胎组织或人胚胎组织的细胞悬液中获得。

公开(公告)号：EP1391505A4

公开(公告)日：2004-09-29

申请号：EP02720586

申请日：2002-04-24

Applicant: AJINOMOTO KK

Inventor： TANIGUCHI HIDEKI ,  SUZUKI ATSUSHI

IPC: A61K35/12 ,  A61P1/18 ,  C12N5/074 ,  C12Q1/68 ,  G01N33/50 ,  C12N5/08 ,  A61K35/39 ,  C12N15/09 ,  C12Q1/02 ,  G01N33/15

CPC classification number: C12Q1/6881 ,  A61K35/12 ,  C12N5/0678 ,  C12N2503/02 ,  C12Q2600/158 ,  G01N33/5005

Abstract: A method of separating pancreatic stem cells from mammalian pancreas; a method of identifying mammalian pancreatic stem cells; and use of the pancreatic stem cells thus separated and identified. More specifically, a method of separating mammalian pancreatic stem cells and a method of identifying the same involving the step of analyzing the expression state of at least two marker proteins selected from the group consisting of c-Met, c-Kit, CD45, TER119 and Flk-1 or genes encoding the same.

公开(公告)号：EP1309673A2

公开(公告)日：2003-05-14

申请号：EP01957508.3

申请日：2001-08-09

Applicant: ES Cell International Pte Ltd.

Inventor： LU, Kuanghui ,  PANG, Kevin ,  RUBIN, Lee

IPC: C12N5/06 ,  C12N5/08

CPC classification number: C12N5/0676 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0647 ,  C12N5/0678 ,  C12N2500/02 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/125 ,  C12N2501/148 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2501/35 ,  C12N2501/39 ,  C12N2501/835 ,  C12N2510/02

Abstract: The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.

Abstract translation: 本发明涉及基本上纯的活胰腺祖细胞群，以及用于分离这些细胞的方法。 本发明进一步涉及这种祖细胞及其子代的某些治疗用途。

公开(公告)号：EP1175487A2

公开(公告)日：2002-01-30

申请号：EP00915758.7

申请日：2000-02-10

Applicant: Curis, Inc.

Inventor： KAGAN, David ,  FUNG, Brenda ,  PANG, Kevin

IPC: C12N5/06 ,  A61K35/39 ,  A61P5/48

CPC classification number: C12N5/0678 ,  A61K35/12 ,  C12N2500/36 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  Y02A50/471

Abstract: The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.