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    METHOD FOR MANUFACTURING PANCREATIC-HORMONE-PRODUCING CELLS
    2.
    发明授权
    METHOD FOR MANUFACTURING PANCREATIC-HORMONE-PRODUCING CELLS 有权

    公开(公告)号：EP2505639B1

    公开(公告)日：2018-09-19

    申请号：EP10841072.1

    申请日：2010-12-28

    Applicant: Takeda Pharmaceutical Company Limited

    Inventor： HOSOYA, Masaki ,  KUNISADA, Yuya ,  SHOJI, Masanobu ,  YAMAZOE, Noriko

    IPC: A61K35/39 ,  G01N33/50 ,  C12N5/071

    CPC classification number: C12N5/0676 ,  A61K35/39 ,  C12N5/0678 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/727 ,  C12N2506/45 ,  G01N33/507

    Abstract: The present invention provides a method of more efficiently producing pancreas cells, particularly pancreatic hormone-producing cells, a method of stably producing pancreas cells in a large amount by more efficiently inducing differentiation of stem cells into pancreas cells, a medicament containing a pancreas cells and a screening method using the cells. A method of producing pancreatic hormone-producing cells, including subjecting stem cells to the following steps (1) - (4): (1) a step of cultivating stem cells in a medium containing an activator of activin receptor-like kinase-4,7 and a GSK3 inhibitor (2) a step of cultivating the cells obtained in the aforementioned step (1) in a medium containing an activator of activin receptor-like kinase-4,7 (3) a step of cultivating the cells obtained in the aforementioned step (2) in a medium containing any one or more kinds selected from the group consisting of (a) retinoic acid receptor agonists, (b) at least one kind selected from the group consisting of inhibitors of AMP-activated protein kinase and/or activin receptor-like kinase-2,3,6, and BMP antagonists, and (c) inhibitors of activin receptor-like kinase-4,5,7 (4) a step of cultivating the cells obtained in the aforementioned stem (3).

    USE OF SUSD2 PROTEIN AS MARKER
    4.
    发明公开
    USE OF SUSD2 PROTEIN AS MARKER 审中-公开
    使用SUSD2蛋白作为标记

    公开(公告)号：EP3178923A1

    公开(公告)日：2017-06-14

    申请号：EP15829144.3

    申请日：2015-08-04

    Applicant: Peking University ,  Peking University Shenzhen Graduate School ,  Beijing Ruipu Chenchuang Technology Co., Ltd

    Inventor： DENG, Hongkui ,  LIU, Haisong ,  ZHU, Dicong ,  YANG, Huan ,  LIANG, Zhen

    IPC: C12N5/071 ,  G01N33/53

    CPC classification number: G01N33/507 ,  C12N5/0676 ,  C12N5/0678 ,  G01N33/5073 ,  G01N33/56966 ,  G01N2500/10

    Abstract: Disclosed is a use of a SUSD2 protein as a marker, in particular a use of the SUSD2 protein as the marker in identification, selection or separation of pancreatic internal secretion precursor cells and/or newborn pancreatic internal secretion cells; and a use of an mRNA, for encoding the SUSD2 protein, of a precursor protein as the marker in identification of the pancreatic internal secretion precursor cells and/or the newborn pancreatic internal secretion cells. Through analysis of gene expression of pancreatic endoderm cells sourced by induced directional differentiation of human pluripotent stem cells, the enrichment expression of a SUSD2 gene in the pancreatic internal secretion precursor cells and the newborn pancreatic internal secretion cells is found. In addition, a protein encoded by the SUSD2 gene is a receptor protein on cell membranes. Using the protein as the marker, the identification, the selection or the separation of the pancreatic internal secretion precursor cells and the newborn pancreatic internal secretion cells can be carried out, which has an important significance on research about pancreas related cells in each development stage.

    Abstract translation: 公开了SUSD2蛋白质作为标志物的用途，特别是SUSD2蛋白质作为胰腺内分泌前体细胞和/或新生胰腺内分泌细胞的鉴定，选择或分离中的标记的用途; 以及用于编码SUSD2蛋白的前体蛋白质作为鉴定胰内分泌前体细胞和/或新生胰腺内分泌细胞的标记的用途。 通过分析由人多能干细胞的诱导定向分化产生的胰腺内胚层细胞的基因表达，发现SUSD2基因在胰内分泌前体细胞和新生胰腺内分泌细胞中的富集表达。 另外，由SUSD2基因编码的蛋白质是细胞膜上的受体蛋白质。 以该蛋白质作为标记物，可以进行胰内分泌前体细胞和新生胰腺内分泌细胞的鉴定，选择或分离，这对于研究每个发育阶段的胰腺相关细胞具有重要意义。

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