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74 条记录 (耗时 0.025 秒)

    RNASE-H-based assays utilizing modified RNA monomers
    32.
    发明公开
    RNASE-H-based assays utilizing modified RNA monomers 有权
    RNASE-H-BASIERTE测定单核苷酸修饰型RNA单体

    公开(公告)号：EP2644708A1

    公开(公告)日：2013-10-02

    申请号：EP13173389.1

    申请日：2009-04-30

    申请人： Integrated Dna Technologies, Inc.

    发明人： Walder, Joseph Alan ,  Behlke, Mark Aaron ,  Rose, Scott ,  Dobosy, Joseph

    IPC分类号： C12Q1/68

    CPC分类号： C12N9/96 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2549/101 ,  C12Q2525/186 ,  C12Q2521/301 ,  C12Q2525/121

    摘要： The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

    摘要翻译： 本发明涉及用于各种生物测定的新型寡核苷酸化合物，例如核酸扩增，连接和测序反应。 该寡核苷酸在寡核苷酸的3'末端或其附近包含核糖核酸结构域和封闭基团。 这些化合物提供了前所未见的增加的特异性水平。 还提供了进行核酸扩增，连接和测序的方法。 此外，本文还公开了含有寡核苷酸的试剂盒。

    Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
    33.
    发明公开
    Methods and compositions for the specific inhibition of gene expression by double-stranded RNA 有权
    Verfahren und Zusammensetzungen zur spezifischen Inhibition der Genexpression durchdoppelsträngigeRNA

    公开(公告)号：EP2514758A1

    公开(公告)日：2012-10-24

    申请号：EP12174021.1

    申请日：2005-03-15

    申请人： City of Hope ,  INTEGRATED DNA TECHNOLOGIES, INC.

    发明人： Rossi, John J. ,  Behlke, Mark A. ,  Kim, Dongho

    IPC分类号： C07H21/04 ,  A61K48/00 ,  C12N15/11 ,  C12N15/68 ,  C12N15/63 ,  C12N15/113

    CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/33 ,  C12N2310/50 ,  C12N2310/51 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2320/51 ,  C12N2330/30

    摘要： The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA ("dsRNA"), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.

    摘要翻译： 本发明涉及用于选择性地降低基因产物从细胞中所需靶基因的表达以及用于治疗由基因表达引起的疾病的组合物和方法。 更具体地，本发明涉及含有双链RNA（“dsRNA”）的组合物及其制备方法，其能够降低真核细胞中靶基因的表达。 dsRNA具有长度为25至约30个核苷酸的第一寡核苷酸序列和在生物学条件下与第一序列退火的第二寡核苷酸序列。 此外，具有至少19个核苷酸的序列长度的dsRNA序列之一的区域与由靶基因产生的RNA的核苷酸序列充分互补，以通过RNAi机制触发靶RNA的破坏。