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公开(公告)号：EP1945808A4
公开(公告)日：2009-09-23
申请号：EP06813323
申请日：2006-08-07
发明人： SORGE JOSEPH A
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/6853 , C12Q2537/137 , C12Q2525/301 , C12Q2521/301 , C12Q2531/119 , C12Q2531/101 , C12Q2525/161
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32.发明公开METHODS FOR DETECTION OF A TARGET NUCLEIC ACID BY FORMING A CLEAVAGE STRUCTURE USING A REVERSE TRANSCRIPTASE 审中-公开METHOD FOR DETECTION靶核酸MEANS形成的一种缝隙构造的作者基于逆转录酶
公开(公告)号：EP2038428A1
公开(公告)日：2009-03-25
申请号：EP07809814.2
申请日：2007-06-22
发明人： SORGE, Joseph, A.
CPC分类号： C12Q1/6823 , C12Q2525/301 , C12Q2525/161 , C12Q2521/307
摘要： The invention relates to and methods for generating a signal indicative of the presence of a target nucleic acid in a sample. The compositions and methods include a reverse transcriptase, a nuclease, an upstream primer and downstream probe.
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33.发明公开SYSTEM AND METHOD FOR FLUORESCENCE EXCITATION AND DETECTION HAVING DISTINCT OPTICAL PATHS 审中-公开系统和方法用于荧光激发和检测具有不同的光路
公开(公告)号：EP1893975A2
公开(公告)日：2008-03-05
申请号：EP06784804.4
申请日：2006-06-12
IPC分类号： G01N21/01
CPC分类号： G01J3/10 , G01J3/4406 , G01J2003/1213 , G01N21/645 , G01N21/6452 , G01N21/6486 , G01N2021/6484 , G01N2201/0446 , G01N2201/0612 , G01N2201/062
摘要： A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).
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公开(公告)号：EP1869217A2
公开(公告)日：2007-12-26
申请号：EP06748510.2
申请日：2006-03-21
申请人： STRATAGENE
CPC分类号： C12Q1/6809 , C12Q1/6816 , C12Q2533/107 , C12Q2545/114 , C12Q2525/207
摘要： The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.
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公开(公告)号：EP1606389A4
公开(公告)日：2007-02-21
申请号：EP04718119
申请日：2004-03-05
发明人： SORGE JOSEPH A , FIRMIN ANDREW
IPC分类号： C12N20060101 , C12N1/00 , C12Q1/68
CPC分类号： C12Q1/6818 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2565/1015 , C12Q2535/125
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公开(公告)号：EP1226255B1
公开(公告)日：2006-03-29
申请号：EP00973960.8
申请日：2000-10-27
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3' to 5' exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
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公开(公告)号：EP1616033A2
公开(公告)日：2006-01-18
申请号：EP04758226.7
申请日：2004-03-19
发明人： BORNS, Michael
CPC分类号： C12N15/102 , C12N9/1252 , C12Q1/6844 , C12Q1/686 , C12Q2527/119 , C12Q2522/101 , C12Q2521/101
摘要： The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
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38.发明公开Thermal cycler including a temperature gradient block 失效温泉Kreisprozessgerätmit Temperaturgraddienst
公开(公告)号：EP1609849A1
公开(公告)日：2005-12-28
申请号：EP05010116.1
申请日：1994-10-20
申请人： STRATAGENE
CPC分类号： B01L7/52 , B01L7/525 , B01L7/5255 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2300/1844 , G01N35/0099 , G01N35/028 , G01N2035/042 , Y10S435/809 , Y10T436/25
摘要： A method in which a temperature is generated across a gradient block, and an apparatus comprising a block (2, 17, 18, 19) across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells (3) on the gradient block (2, 17, 18, 19) can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment, the gradient block (2, 17, 18, 19) is integrated into a thermal cycler used for nucleic acid amplification reactions.
摘要翻译： 一种在梯度块上产生温度的方法，以及包括可以产生温度梯度的块（2,17,18,19）的装置。 通过设置这样的梯度，保留在梯度块（2,17,18,19）上的孔（3）中的多个反应混合物可以在仅稍微不同的温度下同时运行，从而允许反应的最佳温度为 快速识别。 在优选的实施方案中，将梯度嵌段（2,17,18,19）整合到用于核酸扩增反应的热循环仪中。
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公开(公告)号：EP1578951A2
公开(公告)日：2005-09-28
申请号：EP03809647.5
申请日：2003-10-24
IPC分类号： C12N1/00
CPC分类号： C07H21/04 , C12N9/1252 , C12Q1/6869 , C12Q2535/101 , C12Q2521/101
摘要： The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3'-5' exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
摘要翻译： 本发明涉及具有降低的碱基模拟物检测活性的古细菌DNA聚合酶突变体的产生和表征。 本发明进一步提供了具有降低的碱性类似物检测活性的古细菌DNA聚合酶突变体，其含有调节其它DNA聚合酶活性的额外突变，包括DNA聚合或3'-5'外切核酸酶活性。 本发明还公开了具有降低的碱基类似物检测活性的DNA聚合酶的方法和应用。
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公开(公告)号：EP1438430A4
公开(公告)日：2005-05-25
申请号：EP02763814
申请日：2002-10-02
发明人： TAYLOR ROGER H , ORR ALAN R
IPC分类号： G01N21/78 , C12N15/00 , C12P19/34 , C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q2537/165
摘要： The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithms and methods and/systems implementing the algorithms.
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