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206 条记录 (耗时 0.019 秒)

    BIOSENORCHIP
    31.
    发明公开
    BIOSENORCHIP 审中-公开

    公开(公告)号：EP1272850A2

    公开(公告)日：2003-01-08

    申请号：EP01927637.7

    申请日：2001-03-29

    申请人： Infineon Technologies AG

    发明人： FREY, Alexander ,  THEWES, Roland

    IPC分类号： G01N33/543 ,  C12Q1/68

    CPC分类号： G01N33/5438 ,  G01N27/3277

    摘要： The invention relates to a biosensor chip that is provided with a first electrode and a second electrode. The first electrode is provided with a holding area for holding probe molecules which can bind macromolecular biopolymers. The invention also relates to an integrated electric differentiating circuit by means of which an electric current can be detected and can be differentiated according to time, whereby said current is generated during a reduction/oxidation recycling procedure.

    摘要翻译： 本发明涉及一种具有第一电极和第二电极的生物传感器芯片。 第一电极具有用于保持可结合大分子生物聚合物的探针分子的保持区域。 本发明还涉及一种集成的差分电路，通过该集成的差分电路可以检测电流并且可以根据时间来区分电流，由此在还原/氧化再循环过程期间产生所述电流。

    DETECTION SYSTEM
    33.
    发明公开
    DETECTION SYSTEM 审中-公开
    检测系统

    公开(公告)号：EP1234057A2

    公开(公告)日：2002-08-28

    申请号：EP00979768.9

    申请日：2000-11-29

    申请人： The Secretary of State for Defence

    发明人： LEE, Martin, Alan ,  LESLIE, Dario, Lyall

    IPC分类号： C12Q1/68 ,  G01N27/00 ,  B01L7/00 ,  B01L3/00

    CPC分类号： C12Q1/6844 ,  G01N27/3277 ,  C12Q2565/607 ,  C12Q2563/113 ,  C12Q2561/113

    摘要： A method for detecting a target nucleic acid sequence in a sample, by subjecting it to an amplification reaction and taking continuous electrochemical measurements on it during the reaction. The method can be used to determine whether an amplification reaction has taken place, to quantitate the amount of target in the sample or to determine sequence characteristics. Also disclosed is apparatus for use in the method, comprising (i) an amplification reaction vessel which comprises an electrochemical cell, (ii) means for taking continuous electrochemical measurements on a sample contained in the vessel and (iii) temperature control and measurement means, wherein the electrochemical cell comprises an element formed from an electrically conducting plastics material such as a polymer loaded with an electrically conducting material. Further disclosed is a reaction vessel for use in the apparatus, a probe for use in the method and a kit for effecting the method.

    DETECTION OF PURINE AND PYRIMIDINE NUCLEOTIDES AND UNDERIVATIZED NUCLEIC ACIDS BY SINUSOIDAL VOLTAMMETRY
    34.
    发明公开
    DETECTION OF PURINE AND PYRIMIDINE NUCLEOTIDES AND UNDERIVATIZED NUCLEIC ACIDS BY SINUSOIDAL VOLTAMMETRY 失效
    使用正弦形伏安法的嘌呤和嘧啶核苷酸和非衍生化NAKLEINSAÜREN的识别

    公开(公告)号：EP0995111A1

    公开(公告)日：2000-04-26

    申请号：EP98934328.0

    申请日：1998-07-10

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： KUHR, Werner, G. ,  SINGHAL, Pankaj

    IPC分类号： G01N27/26

    CPC分类号： G01N27/3277

    摘要： Sinusoidal voltammetry was employed to detect both purine and pyrimidine-based nucleic acids. Adenine and cytosine, representing these two classes of nucleic acids, could be detected with nanomolar detection limits at a copper electrode under these conditions, where the sensitivity for adenine was much higher than that for cytosine. Detection limits for purine-containing nucleotides (e.g., adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), and adenosine 5'-triphosphate (ATP)) were on the order of 70-200 nM using this method. These detection limits are achieved for native nucleotides and are over two orders of magnitude lower than those found with UV absorbance detection. Pyrimidine-based nucleotides could also be detected with high sensitivity due to the presence of a sugar backbone which is electroactive at the copper surface.