摘要:
Ein Verfahren zur Herstellung einer in einen Probenbehälter (1, 1a, 1b, 1c) eingeschlossenen, wasserhaltigen, gefrierkonservierten Probe, bei welchem zumindest Probenmaterial in den Probenbehälter eingebracht, dieser sodann druckdicht verschlossen und anschließend mittels eines Kryogens (8) in einer zeitlichen und örtlichen Abfolge abgekühlt wird, wobei vorerst ein Opferbereich (4) des Behälterinhaltes (2) und erst danach der gesamte Behälterinhalt erstarrt. Ein hierbei bevorzugt verwendbarer Probenbehälter (1a) ist röhrchenförmig, an seinen beiden Enden verschlossen und im Wesentlichen U-förmig ausgebildet.
摘要:
A system and method for concurrently and uniformly removing thermal energy from a specimen sample. A thermal insulating device is provided comprising an insulating material, the device having a plurality of chambers for receiving specimen samples, the device further includes a thermal ballast whereby the rate of thermal energy removal is controlled and influenced by the thermal ballast.
摘要:
A single-use coring probe for collecting a frozen aliquot from a frozen biological sample includes a hollow coring bit and an ejector adapted. The ejector is operable to eject a frozen sample core from the bit as it moves from a retracted position to an extended position. Use of the ejector converts the probe to a disabled configuration to discourage reuse of the coring probe to obtain another sample. The probe may include a locking mechanism adapted to prevent re-use of the single-use coring probe by locking the ejector in the extended position. A hand-held coring device can be used to take frozen sample cores from frozen samples. A tissue container is suitable for holding a frozen tissue sample in frozen storage and also for holding the sample while the sample is sectioned and/or a full-depth frozen sample core is extracted from the frozen tissue.
摘要:
The invention relates to a method of inspecting a sample (101), the method comprising: repeatedly • Preparing a fresh surface (304,306) on the block face of a sample by removing the surface layer, • Inspecting the block face with a scanning probe microscope (100), resulting in information of the current surface layer,
Followed by • Combining the information of a multitude of inspections into a 3D reconstruction of the sample,
Characterized in that The removal is performed in an evacuated environment by ion beam milling.
By performing the removal of the surface with ion beam milling, mechanical deformations are avoided, surface roughness is reduced, and the vertical resolution is enhanced, resulting in a better 3D reconstruction of the sample.