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    METHOD AND KIT FOR QUANTITATIVE DETERMINATION FOR SMALL, DENSE PARTICLE LOW DENSITY LIPOPROTEINS
    31.
    发明公开
    METHOD AND KIT FOR QUANTITATIVE DETERMINATION FOR SMALL, DENSE PARTICLE LOW DENSITY LIPOPROTEINS 有权
    方法和试剂盒用于定量测定一种小块低密度脂蛋白

    公开(公告)号：EP1930442A4

    公开(公告)日：2008-09-10

    申请号：EP06797203

    申请日：2006-08-31

    申请人： DENKA SEIKEN KK

    发明人： ITOH YASUKI

    IPC分类号： C12Q1/60 ,  G01N33/92

    CPC分类号： C12Q1/60 ,  G01N33/92

    摘要： Disclosed is a method for performing separation/determination of a small, dense LDL rapidly in a simple manner without the need of any pre-treatment of a sample, which is applicable to an automatic analyzer. A method for quantification of a small, dense LDL cholesterol comprising the steps of mixing an enzyme for determination of cholesterol with a sample in the presence of a polyoxyethylene-polyoxypropylene copolymer or a derivative thereof to allow the copolymer or derivative to react with the small, dense LDL selectively, among other lipoproteins, and determining the amount of cholesterol produced by the reaction.

    NOVEL POLYMER AND METHOD OF MEASURING CHOLESTEROL THEREWITH
    33.
    发明公开
    NOVEL POLYMER AND METHOD OF MEASURING CHOLESTEROL THEREWITH 有权
    消除聚合物VERFAHREN ZUR MESSUNG VON CHOLESTERIN DAMIT

    公开(公告)号：EP1903069A1

    公开(公告)日：2008-03-26

    申请号：EP06729024.7

    申请日：2006-03-14

    申请人： Wako Pure Chemical Industries, Ltd.

    发明人： ITAI, Tomokazu ,  MORI, Kiyoko ,  YAGURA, Yo ,  KOYAMA, Isao ,  YAMAMOTO, Naoyuki

    IPC分类号： C08G81/00 ,  C08F4/04 ,  C08F293/00 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/68 ,  G01N33/92

    CPC分类号： G01N33/92 ,  C08F210/00 ,  C08F293/00 ,  C08F293/005 ,  G01N2800/044

    摘要： It is an object of the present invention to provide the method for determining cholesterol in lipoprotein and reagent for determining cholesterol comprising the polymer, and the present invention relates to the reagent for determining cholesterol in lipoproteins such as high-density lipoprotein (HDL), low-density lipoprotein(LDL) and very low-density lipoprotein(VLDL) and the determination method using the polymer comprising the following units as constituents:
    (iv) a polyethylene glycol segment represented by the general formula [1]:

            (CH 2 CH 2 O)k-     [1]

    (wherein, k represents an integer of 10 to 250);
    (v) a monomer unit represented by the general formula [2]:

    (wherein R 1 represents a hydrogen atom or C 1 -C 3 alkyl group); and
    (vi) a monomer unit represented by the general formula [3]:

    [wherein, R 2 represents a hydrogen atom or C 1 -C 3 alkyl group, and R 3 represents a group represented by the general formula [4]:

            -COOR 4      [4]

    (wherein, R 4 represents an alkyl group, a haloalkyl group or a bornyl group), an alkyl group, an alkoxy group, an aralkyl group or an alkylcarbamoyl group; particularly, in the direct measurement of cholesterols such as HDL, LDL and VLDL contained in the sample, the reagent for determining cholesterol in the presence of said polymer, and the method for determining the concentration of cholesterol in a specific lipoprotein using said reagent for determining cholesterol, and a novel polymer.

    摘要翻译： 本发明的目的是提供用于测定脂蛋白中的胆固醇的方法和用于测定包含该聚合物的胆固醇的试剂，本发明涉及用于测定脂蛋白中的胆固醇的试剂，例如高密度脂蛋白（HDL），低密度脂蛋白 密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）以及使用包含以下单位的聚合物作为成分的测定方法：（iv）由通式[1]表示的聚乙二醇片段：€ƒ€€ ƒ€ƒ€ƒ€ƒ€ƒ（CH 2 CH 2 O）k-€ƒ€ƒ€ƒƒ[1]（其中，k表示10到250的整数）; （v）由通式[2]表示的单体单元：（其中R 1表示氢原子或C 1 -C 3烷基）; 和（vi）由通式[3]表示的单体单元：[其中，R 2表示氢原子或C 1〜C 3烷基，R 3表示由通式[4]表示的基团： ƒ€ƒ€ƒ€ƒ€€ƒ€ƒ-COOR 4€ƒ€ƒ€ƒ€ƒ[4]（其中，R 4表示烷基，卤代烷基或冰基）， 烷基，烷氧基，芳烷基或烷基氨基甲酰基; 特别是直接测定样品中所含的HDL，LDL，VLDL等胆固醇的方法，在所述聚合物存在下，测定胆固醇的试剂，以及使用所述试剂测定特定脂蛋白中的胆固醇浓度的方法 胆固醇和新型聚合物。

    METHOD OF ANALYZING COMPONENTS IN BIOLOGICAL SAMPLES
    36.
    发明公开
    METHOD OF ANALYZING COMPONENTS IN BIOLOGICAL SAMPLES 有权
    程序用于生物样品成分分析

    公开(公告)号：EP1288306A4

    公开(公告)日：2006-05-10

    申请号：EP01934540

    申请日：2001-06-05

    申请人： SYSMEX CORP

    发明人： KISHI KOJI ,  KAKUYAMA TSUTOMU ,  OCHIAI KOJI

    IPC分类号： C12Q1/44 ,  C12Q1/60 ,  G01N33/92

    CPC分类号： C12Q1/44 ,  C12Q1/60 ,  G01N33/92 ,  Y10S435/975

    摘要： A method of selectively assaying components (in particular, cholesterol) in very low-density lipoprotein (VLDL) which is one of serum lipoproteins. In this assay, an enzyme reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE), which are enzymes reacting well with high-density lipoprotein (HDL) and VLDL, is carried out in the presence of calixalene or its salt optionally together with one or more substances selected from among albumin and basic amino acids.

    METHOD AND REAGENT FOR MEASURING CHOLESTEROL IN HIGH DENSITY LIPOPROTEINS
    38.
    发明公开
    METHOD AND REAGENT FOR MEASURING CHOLESTEROL IN HIGH DENSITY LIPOPROTEINS 有权
    VERFAHREN UND REAGENS ZUR MESSUNG VON CHOLESTERIN LIPOPROTEINEN HOHER DICHTE

    公开(公告)号：EP1555326A1

    公开(公告)日：2005-07-20

    申请号：EP03756656.9

    申请日：2003-10-16

    申请人： Kyowa Medex Co., Ltd.

    发明人： KATAYAMA, Yuki Kyowa Medex Co., Ltd. ,  FUJINAKA, Mayumi Kyowa Medex Co., Ltd.

    IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92

    CPC分类号： G01N33/92 ,  C09B21/00 ,  C12Q1/60 ,  G01N2333/90241 ,  G01N2333/904 ,  G01N2333/914

    摘要： A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of a surfactant selected from the group consisting of polyoxyethylene alkylamine or polyoxyethylene alkenylamine and a surfactant selected from the group consisting of polyoxyethylene polycyclic phenyl ether sulfate and an anionic bile acid derivative, and measuring the formed hydrogen peroxide or a reduced coenzyme; and a reagent used therefor.

    摘要翻译： 一种用于定量测定样品中高密度脂蛋白胆固醇的方法，其包括：将样品与i）胆固醇酯酶和胆固醇氧化酶或ii）胆固醇酯酶，氧化型辅酶和胆固醇脱氢酶在水性介质中反应，所述方法包括i）非离子表面活性剂 ，聚阴离子和白蛋白，或ii）选自聚氧乙烯烷基胺或聚氧乙烯链烯基胺的表面活性剂和选自聚氧乙烯多环苯基醚硫酸盐和阴离子胆汁酸衍生物的表面活性剂的组合，并测量形成的过氧化氢 或还原型辅酶; 和用于其的试剂。