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    Method and kit for measuring a component to be assayed in a specimen
    2.
    发明授权
    Method and kit for measuring a component to be assayed in a specimen 有权
    用于测量样品中待测组分的方法和试剂盒

    公开(公告)号：EP2495565B1

    公开(公告)日：2017-09-20

    申请号：EP10826752.7

    申请日：2010-10-27

    申请人： Kyowa Medex CO., LTD.

    发明人： KAWAMURA, Mizuho

    IPC分类号： G01N33/531 ,  G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/6866

    摘要： Methods and kits for measuring a component to be measured in a specimen are provided, which enable accurate measurements that are not affected by reaction temperatures or such when measuring the component to be measured, such as antigens. Methods for measuring a component to be measured, which comprise: reacting, in the presence of a fatty acid alkanolamide, a component to be measured in a specimen with a first antibody which binds to the component to be measured; then reacting a labeled second antibody, in which a label is bound to a second antibody that binds to the component to be measured, to form an immunocomplex comprising the first antibody, the component to be measured, and the labeled second antibody; and measuring the amount of the label in the formed immunocomplex; and kits for measuring the component to be measured in a specimen used in the measurement methods.

    摘要翻译： 提供了用于测量样本中要测量的部件的方法和套件，其能够进行准确的测量，这些测量不受反应温度等的影响，或者当测量诸如抗原的待测量的部件时。 测定被测定成分的方法，其特征在于，在脂肪酸链烷醇酰胺的存在下，使试样中的测定对象成分与结合于被测定成分的第一抗体反应， 然后使标记的第二抗体与结合待测量的组分的第二抗体结合以形成包含第一抗体，待测量的组分和标记的第二抗体的免疫复合物; 并测量形成的免疫复合物中标记的量; 以及用于测量在测量方法中使用的样本中待测量的部件的套件。

    METHOD FOR STABILIZING ASCORBIC ACID OXIDASE
    7.
    发明公开
    METHOD FOR STABILIZING ASCORBIC ACID OXIDASE 有权
    的稳定化方法抗坏血酸

    公开(公告)号：EP2857503A4

    公开(公告)日：2016-01-13

    申请号：EP13794306

    申请日：2013-05-23

    申请人： KYOWA MEDEX CO LTD

    发明人： KINJO KENTA ,  ARATAKE TOMOKO

    IPC分类号： C12N9/02 ,  C12N9/96

    CPC分类号： C12N9/96 ,  C12N9/0063 ,  C12Q1/26 ,  C12Y110/03003 ,  G01N2333/90235

    摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.

    METHOD FOR MEASURING CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN
    9.
    发明公开
    METHOD FOR MEASURING CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN 审中-公开
    VERFAHREN ZUR MESSUNG VON CHOLESTERIN在HOCHDICHTEM LIPOPROTEIN

    公开(公告)号：EP2891718A1

    公开(公告)日：2015-07-08

    申请号：EP13833253.1

    申请日：2013-08-30

    申请人： Kyowa Medex Co., Ltd.

    发明人： SASAKI, Rie ,  KATAYAMA, Yuki

    IPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/60

    CPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/902 ,  G01N2333/904 ,  G01N2333/914 ,  G01N2333/918

    摘要： Provided is a method for measuring cholesterol in high-density lipoprotein (HDL) simply and accurately. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising at least one substance selected from the group consisting of pyridinium salts and quaternary ammonium salts each having a specific structure, and a polyanion to form hydrogen peroxide or reduced coenzyme and measuring the formed hydrogen peroxide or reduced coenzyme.

    摘要翻译： 提供了一种简单准确地测定高密度脂蛋白（HDL）胆固醇的方法。 一种用于测量样品中高密度脂蛋白胆固醇的方法，包括使样品与i）胆固醇酯水解酶和胆固醇氧化酶或ii）胆固醇酯水解酶，氧化型辅酶和胆固醇脱氢酶在水溶液 包含选自吡啶鎓盐和各自具有特定结构的季铵盐的至少一种物质的介质和形成过氧化氢或还原型辅酶的聚阴离子并测量形成的过氧化氢或还原型辅酶。

    METHOD FOR ANALYSIS FOR COMPONENT TO BE ASSAYED
    10.
    发明公开
    METHOD FOR ANALYSIS FOR COMPONENT TO BE ASSAYED 有权
    分析方法，供审议的组件

    公开(公告)号：EP2653551A4

    公开(公告)日：2014-05-14

    申请号：EP11849227

    申请日：2011-12-12

    申请人： KYOWA MEDEX CO LTD

    发明人： MURAKAMI TOMOMI

    IPC分类号： C12Q1/00 ,  C12Q1/28 ,  G01N21/77 ,  G01N21/78 ,  G01N33/64

    CPC分类号： G01N21/77 ,  C09B21/00 ,  C12Q1/28 ,  G01N21/78 ,  G01N2333/908

    摘要： The present invention provides a method for measuring a component to be measured in a sample, comprising converting the component to be measured in the sample into hydrogen peroxide and measuring the formed hydrogen peroxide in the presence of an ±-keto acid using an oxidative-coloring chromogen. The present invention also provides a method for suppressing the influence of a peroxide on a method of converting a component to be measured in a sample into hydrogen peroxide and measuring the formed hydrogen peroxide using an oxidative-coloring chromogen, the suppression method comprising using an ±-keto acid. The measuring method and the suppression method of the present invention using an ±-keto acid suppress the influence of a peroxide to give an accurate measurement of the component to be measured in the sample.