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    METHODS AND MEANS FOR OBTAINING MODIFIED PHENOTYPES
    42.
    发明公开
    METHODS AND MEANS FOR OBTAINING MODIFIED PHENOTYPES 审中-公开
    获得修饰表型的方法和手段

    公开(公告)号：EP3214177A3

    公开(公告)日：2017-11-22

    申请号：EP17162147.7

    申请日：1999-04-07

    申请人： Commonwealth Scientific and Industrial Research Organisation

    发明人： WATERHOUSE, Peter, Michael ,  WANG, Ming-Bo ,  GRAHAM, Michael, Wayne

    IPC分类号： C12N15/82 ,  C12N15/11

    CPC分类号： C12N15/8283 ,  C12N15/63 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8282

    摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly plant cells, by introducing a chimeric RNA molecule comprising at least one RNA region with a nucleotide sequence comprising
    i. a sense nucleotide sequence of at least 15 consecutive nucleotides having 100% sequence identity with at least part of the nucleotide sequence of the nucleic acid of interest; and
    ii. an antisense nucleotide sequence including at least 15 consecutive nucleotides, having about 100% sequence identity with the complement of said at least 15 consecutive nucleotides of said sense nucleotide sequence;
    wherein said RNA region is capable of forming an artificial hairpin RNA structure with a double stranded RNA stem by base-pairing between the regions with sense and antisense nucleotide sequence such that said at least 15 consecutive nucleotides of the sense sequence basepair with said at least 15 consecutive nucleotides of the antisense sequence, wherein said nucleic acid of interest is a gene integrated into the genome of said eukaryotic cell, wherein said RNA molecule is produced by transcription of a chimeric gene.

    摘要翻译： 提供了用于通过将包含至少一个RNA区的嵌合RNA分子与包含i的核苷酸序列导入来减少真核细胞，特别是植物细胞中感兴趣的核酸的表型表达的方法和手段。 与感兴趣的核酸的核苷酸序列的至少一部分具有100％序列同一性的至少15个连续核苷酸的正义核苷酸序列; 和ii。 反义核苷酸序列，其包含至少15个连续核苷酸，与所述有义核苷酸序列的所述至少15个连续核苷酸的互补序列具有约100％的序列同一性; 其中所述RNA区域能够通过具有有义和反义核苷酸序列的区域之间的碱基配对形成具有双链RNA干扰的人造发夹RNA结构，使得有义序列碱基对的所述至少15个连续核苷酸与所述至少15个 其中所述感兴趣的核酸是整合到所述真核细胞的基因组中的基因，其中所述RNA分子通过嵌合基因的转录产生。

    METHODS AND MEANS FOR OBTAINING MODIFIED PHENOTYPES
    44.
    发明公开
    METHODS AND MEANS FOR OBTAINING MODIFIED PHENOTYPES 审中-公开

    公开(公告)号：EP3214177A2

    公开(公告)日：2017-09-06

    申请号：EP17162147.7

    申请日：1999-04-07

    申请人： Commonwealth Scientific and Industrial Research Organisation

    发明人： WATERHOUSE, Peter, Michael ,  WANG, Ming-Bo ,  GRAHAM, Michael, Wayne

    IPC分类号： C12N15/82 ,  C12N15/11

    CPC分类号： C12N15/8283 ,  C12N15/63 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8282

    摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly plant cells, by introducing a chimeric RNA molecule comprising at least one RNA region with a nucleotide sequence comprising
    i. a sense nucleotide sequence of at least 15 consecutive nucleotides having 100% sequence identity with at least part of the nucleotide sequence of the nucleic acid of interest; and
    ii. an antisense nucleotide sequence including at least 15 consecutive nucleotides, having about 100% sequence identity with the complement of said at least 15 consecutive nucleotides of said sense nucleotide sequence;
    wherein said RNA region is capable of forming an artificial hairpin RNA structure with a double stranded RNA stem by base-pairing between the regions with sense and antisense nucleotide sequence such that said at least 15 consecutive nucleotides of the sense sequence basepair with said at least 15 consecutive nucleotides of the antisense sequence, wherein said nucleic acid of interest is a gene integrated into the genome of said eukaryotic cell, wherein said RNA molecule is produced by transcription of a chimeric gene.