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179 条记录 (耗时 0.029 秒)

    Enzymatic oil-degumming method
    41.
    发明公开
    Enzymatic oil-degumming method 审中-公开
    EnzymatischesÖlentschleimungsverfahren

    公开(公告)号：EP2275522A2

    公开(公告)日：2011-01-19

    申请号：EP10175653.4

    申请日：2005-07-18

    申请人： Danisco A/S

    发明人： Miasnikov, Andrei ,  Soe, Jørn ,  Mikkelsen, Jørn ,  Povelainen, Mira ,  Pitkanen, Virve

    IPC分类号： C11B3/00 ,  C11C3/00 ,  C07F9/10 ,  A23J7/00 ,  C12N9/20 ,  C12P7/64

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： The invention also encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of:
    a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3;
    a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and
    a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.

    摘要翻译： 本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂转化为酰基受体。 优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。 第4,5,7,8,12,14或16号或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,9,13,15或17所示的核苷酸序列 或与其具有至少70％同一性的nulceotide seqonunce。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂肪分解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基 如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可以由选自以下的核酸编码：包含SEQ ID No.3所示的核苷酸序列的核酸; 通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

    Lipolytic Enzyme. Uses Thereof In The Food Industry
    42.
    发明公开
    Lipolytic Enzyme. Uses Thereof In The Food Industry 审中-公开
    Lipolytisches Enzym Verwendungen davon in der Lebensmittelindustrie

    公开(公告)号：EP2267107A2

    公开(公告)日：2010-12-29

    申请号：EP10174040.5

    申请日：2005-07-18

    申请人： Danisco A/S

    发明人： Miasnikov, Andrei ,  Soe, Jørn ,  Mikkelsen, Jørn ,  Povelainen, Mira ,  Pitkanen, Virve

    IPC分类号： C11B3/00 ,  A23J7/00 ,  C12N9/20

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of:
    a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3;
    a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and
    a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.

    摘要翻译： 本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂转化为酰基受体。 优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。 第4,5,7,8,12,14或16号或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,9,13,15或17所示的核苷酸序列 或与其具有至少70％同一性的nulceotide seqonunce。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂肪分解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基 如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可以由选自以下的核酸编码：包含SEQ ID No.3所示的核苷酸序列的核酸; 通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

    METHOD FOR PRODUCTION OF DHA-CONTAINING PHOSPHOLIPID THROUGH MICROBIAL FERMENTATION
    46.
    发明公开
    METHOD FOR PRODUCTION OF DHA-CONTAINING PHOSPHOLIPID THROUGH MICROBIAL FERMENTATION 有权
    发酵的磷脂磷酸二氢叶酸发酵液（Verfahren zur Herstellung von DHA-haltigem Phospholipid durch mikrobielle Fermentation

    公开(公告)号：EP2163641A1

    公开(公告)日：2010-03-17

    申请号：EP08763991.0

    申请日：2008-06-03

    申请人： National University Corporation Hokkaido University ,  Research Of Microbes Co., Ltd.

    发明人： OKUYAMA, Hidetoshi ,  ORIKASA, Yoshitake ,  NISHIDA, Takanori

    IPC分类号： C12P9/00

    CPC分类号： C12P7/6481 ,  C12N1/10 ,  C12R1/90

    摘要： Disclosed is a method for producing a DHA phospholipid comprising an ω3 unsaturated fatty acid, particularly DHA, as a constituent lipid by using a microorganism in a simpler manner. Specifically disclosed is a method for producing a phospholipid comprising an ω3 unsaturated fatty acid as a constituent lipid, which comprises the steps of: growing a microorganism capable of producing the ω3 unsaturated fatty acid in a culture medium containing a carbon source; and further culturing the grown microorganism in a culture medium without any carbon source. The method enables to produce a highly value-added phospholipid which comprises an ω3 unsaturated fatty acid as a constituent lipid by using a microorganism capable of producing the ω3 unsaturated fatty acid in a large quantity.

    摘要翻译： 公开了通过以更简单的方式使用微生物来制备包含É3不饱和脂肪酸，特别是DHA作为组成脂质的DHA磷脂的方法。 具体公开了一种生产包含É3不饱和脂肪酸作为构成脂质的磷脂的方法，其包括以下步骤：在含有碳源的培养基中培养能够产生É3不饱和脂肪酸的微生物; 并且在没有任何碳源的培养基中进一步培养生长的微生物。 该方法能够通过使用能够大量生产É3不饱和脂肪酸的微生物来产生包含É3不饱和脂肪酸作为构成脂质的高附加值磷脂。

    Production of polyunsaturated fatty acids using novel cell treatment method
    47.
    发明公开
    Production of polyunsaturated fatty acids using novel cell treatment method 审中-公开
    Herstellung mehrfachungesättigterFettsäurenmittels eines neuartigen Zellbehandlungsverfahrens

    公开(公告)号：EP2130923A2

    公开(公告)日：2009-12-09

    申请号：EP09010790.5

    申请日：2006-02-08

    申请人： Suntory Holdings Limited

    发明人： Higashiyama, Kenichi ,  Nakajima, Toshiharu

    IPC分类号： C12P7/64 ,  C12N1/00 ,  C12P1/00 ,  C12P1/02 ,  C12N1/04 ,  C12N1/14 ,  C12N1/12 ,  A61K31/00 ,  A23L1/00 ,  A23K1/00 ,  C11B1/00

    CPC分类号： A23D9/00 ,  A23K10/12 ,  A23K20/158 ,  A23L33/115 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/005 ,  C12P7/6472 ,  C12P7/6481 ,  A23V2250/1882

    摘要： A method for isolating one compound or more than one compound from a biomass which contains microorganisms that have produced the compound or compounds, the method comprising the following steps: (a) preparing or obtaining wet cells having an average moisture content of between 30% and 80%; (b) subjecting the wet cells to primary drying to obtain primary dried cells having an average moisture content of between 5% and 50%; (c) subjecting the primary dried cells obtained in (b) to secondary drying to obtain secondary dried cells having an average moisture content of no greater than 10%; and (d) extracting or isolating, purifying and/or refining the compound or each of the compounds from the secondary dried cells obtained in (c).

    摘要翻译： 一种从含有生成化合物或微生物的微生物的生物质中分离一种化合物或一种以上化合物的方法，该方法包括以下步骤：（a）制备或获得平均含水量在30％至 80％; （b）使湿细胞进行一次干燥以获得平均含水量在5％至50％之间的初级干燥细胞; （c）使（b）中获得的初级干燥细胞二次干燥，得到平均含水量不大于10％的二次干燥细胞; 和（d）从（c）中获得的二次干燥细胞提取或分离，纯化和/或精制化合物或每种化合物。

    NOVEL PHOSPHOLIPID PROCESSING AGENT
    49.
    发明公开
    NOVEL PHOSPHOLIPID PROCESSING AGENT 审中-公开
    消除磷脂维生素A

    公开(公告)号：EP1918371A1

    公开(公告)日：2008-05-07

    申请号：EP06781181.0

    申请日：2006-07-18

    申请人： Asahi Kasei Pharma Corporation

    发明人： KOGA, Shinji ,  IMAMURA, Shigeyuki

    IPC分类号： C12N15/00 ,  A23L1/30 ,  A61K38/00 ,  C12N9/16 ,  C12N15/09 ,  C12P9/00 ,  C12P19/44

    CPC分类号： C12N9/20 ,  A23L29/10 ,  A61K38/00 ,  C12N9/16 ,  C12P7/6481 ,  C12P9/00 ,  C12P19/44

    摘要： Problems: It is an object of the present invention to provide a novel phospholipase B (PLB) having less hydrolytic activity on phosphatidylinositol (PI) among diacyl form of the phospholipid. Also, it is an object of the invention to provide a method of efficiently manufacturing PI and glyceryl phosphoryl choline (GPC) from a phospholipid mixture by using a substrate specificity of an enzyme.
    Means to Solve the Problems: Provided is a phospholipid processing agent containing an enzyme, which acts effectively on a phospholipid other than PI, such as phosphatidyl choline (lecithin), and having less PLB activity on PI.

    摘要翻译： 问题：本发明的目的是提供一种在二酰基形式的磷脂中对磷脂酰肌醇（PI）具有较少水解活性的新型磷脂酶B（PLB）。 此外，本发明的目的是提供一种通过使用酶的底物特异性从磷脂混合物有效地制备PI和甘油磷酰胆碱（GPC）的方法。 解决问题的手段：提供了含有酶的磷脂加工剂，其有效地作用于PI以外的磷脂，例如磷脂酰胆碱（卵磷脂），并且对PI具有较小的PLB活性。