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179 条记录 (耗时 0.031 秒)

    Enzymatic oil-degumming method
    7.
    发明公开
    Enzymatic oil-degumming method 审中-公开
    酶促油脱胶法

    公开(公告)号：EP2275522A3

    公开(公告)日：2011-09-21

    申请号：EP10175653.4

    申请日：2005-07-18

    申请人： Danisco A/S

    发明人： Miasnikov, Andrei ,  Soe, Jørn ,  Mikkelsen, Jørn ,  Povelainen, Mira ,  Pitkanen, Virve

    IPC分类号： C11B3/00 ,  C11C3/00 ,  C07F9/10 ,  A23J7/00 ,  C12N9/20 ,  C12P7/64 ,  A21D8/04

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： The invention also encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of:
    a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3;
    a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and
    a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.

    摘要翻译： 本发明还包括在各种方法和用途中可使用从下列属之一获得的脂解酶：链霉菌属，棒状杆菌属和嗜热菌属，其中所述脂解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂酰基受体。 优选地，用于这些方法和用途的脂解酶包含如SEQ.ID.NO.1中任一所示的氨基酸序列。 ID。 编号为4,5,7,8,12,14或16的氨基酸序列或与其具有至少70％同一性的氨基酸序列，或者包含如SEQ ID No.3,6,9,13,15或17所示的核苷酸序列 或与其具有至少70％同一性的核苷酸序列。 本发明还涉及能够水解至少半乳糖脂或能够将酰基从半乳糖脂转移到一个或多个酰基受体底物的脂肪分解酶，其中所述酶可以从链霉菌种获得并且包括含有氨基酸的脂解酶 如SEQ ID No.4所示的氨基酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可由选自以下的核酸编码：包含SEQ ID No.3所示核苷酸序列的核酸; 通过遗传密码的变性与核苷酸序列SEQ ID No.3相关的核酸; 和包含与SEQ ID No.3中所示的核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

    Processes for the preparation of phosphatide salts
    8.
    发明公开
    Processes for the preparation of phosphatide salts 审中-公开
    Verfahren zur Herstellung von Phosphatidsalzen

    公开(公告)号：EP2354238A1

    公开(公告)日：2011-08-10

    申请号：EP10154488.0

    申请日：2010-02-24

    申请人： Lipogen Ltd.

    发明人： Rutenberg, David

    IPC分类号： C12P7/64 ,  C12P9/00 ,  C12P13/06

    CPC分类号： C12P7/6481 ,  C12P9/00 ,  C12P13/06

    摘要： The present invention discloses processes a process for the preparation of phosphatide-salt complexes, the process including the steps of: using at least one raw material lecithin as a substrate; and enzymatically processing at least one raw material lecithin with phospholipase-D, racemic or enantiomerically-pure serine, and/or amine in an aqueous carboxylate-salt-complex solution, wherein the step of processing is performed in a single-phase reaction environment, to produce phosphatide-salt complexes (including a mixture of phosphatidic-acid-salt (PA-salt) complex and phosphatidylserine-salt (PS-salt) complex) having a structural fatty-acid chain derived from at least one raw material lecithin. Preferably, the step of processing is performed at a pH in the range of about 4.5-8.0 at a temperature in the range of about 25-60°C. Preferably, the aqueous carboxylate-salt-complex solution is formed from an aqueous solution of a carboxylic acid with a chain length of C2-C8 and a salt in an approximately 1:2 (weight per weight) acid-to-salt ratio.

    摘要翻译： 本发明公开了制备磷脂 - 盐络合物的方法的方法，该方法包括以下步骤：使用至少一种原料卵磷脂作为底物; 并在羧酸盐 - 盐 - 络合物溶液中用磷脂酶-D，外消旋或对映体纯的丝氨酸和/或胺酶处理至少一种原料卵磷脂，其中处理步骤在单相反应环境中进行， 产生具有衍生自至少一种原料卵磷脂的结构脂肪酸链的磷脂 - 盐配合物（包括磷脂酸 - 盐（PA-盐）复合物和磷脂酰丝氨酸盐（PS-盐）复合物的混合物）。 优选地，在大约25-60℃的温度下，在约4.5-8.0的pH范围内进行处理步骤。 优选地，羧酸盐 - 盐络合物溶液由链长为C 2 -C 8的羧酸水溶液和约1:2（重量/重量/重量）酸 - 盐比的盐形成。