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52 条记录 (耗时 0.016 秒)

    Sensitive immunoassays of antigens or antibodies sequestered with immune complexes
    43.
    发明公开
    Sensitive immunoassays of antigens or antibodies sequestered with immune complexes 失效
    敏感的免疫隔离在抗原或抗体的免疫复合物。

    公开(公告)号：EP0080109A1

    公开(公告)日：1983-06-01

    申请号：EP82110331.4

    申请日：1982-11-09

    申请人： New York Blood Center, Inc.

    发明人： Neurath, A. Robert

    IPC分类号： G01N33/54 ,  G01N33/56

    CPC分类号： G01N33/544 ,  G01N33/54306 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/5761 ,  Y10S435/962 ,  Y10S436/804 ,  Y10S436/825 ,  Y10S436/828

    摘要： A process for determining the presence of an antigen or antibody in a sample wherein said antigen or antibody exists in the form of an immune complex which comprises:

    A. contacting the immune complex originating from the sample suspected of containing immune complex with a dissociating buffer whereby said immune complex, if present, is dissociated into antigen and antibody;
    B. contacting a solid support which binds proteins with said dissociating buffer suspected of containing antigen or antibody and removing said buffer;
    C. washing said solid support;
    D. adding protein to fill unoccupied sites on said solid support;
    E. adding radioactively labeled or enzyme labeled antibody or antigen to said support, said labeled antibody or antigen corresponding to antigen or antibody on said solid support, incubating the resultant mass and washing the same;
    F. measuring the radioactivity or enzymatic activity associated with the solid support.

    Peptides for use in determining serum thymic factor
    48.
    发明公开
    Peptides for use in determining serum thymic factor 失效
    精华精华精华精华精华版。

    公开(公告)号：EP0043247A1

    公开(公告)日：1982-01-06

    申请号：EP81302874.3

    申请日：1981-06-25

    申请人： KUREHA KAGAKU KOGYO KABUSHIKI KAISHA

    发明人： Yanaihara, Noboru ,  Konno, Koji

    IPC分类号： C07C103/52 ,  G01N33/56

    CPC分类号： C07K7/062 ,  G01N33/534 ,  G01N33/6863 ,  Y10S930/18

    摘要： Serum thymic factor (STF) occurs in minute amounts in blood, making its determination difficult. It has been found that peptides having a primary structure represented by the formula: wherein

    (1) X is H-Tyr-Gln-Ala-Lys and Y is a hydroxyl group,
    (2) X is pGlu-Ala-Lys(N e- Tyr-H) and Y is a hydroxyl group, or

    (3) X is pGlu-Ala-Lys and Y is TyrOH, can be radioactively labelled and used to determine STF. These peptides can be synthesized by bonding a predetermined amino acid or peptide to the N-end of H-Asn-OH, H-Asn-Tyr-OH or a peptide of the appropriate primary structure and terminating in -Asn-OH or -ASn-Tyr-OH at its C-end without protecting the carboxyl group at the C-end of said H-Asn-OH, H-Asn-Tyr-OH or peptide terminating in -Asn-OH or -Asn-Tyr-OH at its C-end, and as desired elongating the resulting peptide chain from the N-end by the bonding of further predetermined amino acid(s) and/or peptide(s), to obtain the desired peptide.

    摘要翻译： 血清胸腺因子（STF）在血液中发生微量，难以确定。 已经发现，具有由下式表示的一级结构的肽：X-Ser-Gln-Gly-Gly-Ser-Asn-Y（I）...其中...（1）X是H-Tyr-Gln-Ala- Lys和Y是羟基，...（2）X是pGlu-Ala-Lys（Nε-Tyr-H），Y是羟基，或...（3）X是pGlu-Ala-Lys和Y 是TyrOH，可以放射性标记并用于确定STF。 这些肽可以通过将预定的氨基酸或肽键合到H-Asn-OH，H-Asn-Tyr-OH的N末端或适当的一级结构的肽并且终止于-Asn-OH或-ASn -Tyr-OH，而不保护所述H-Asn-OH，H-Asn-Tyr-OH的C末端的羧基或终止于-Asn-OH或-Asn-Tyr-OH的肽 其C-末端，并且根据需要通过进一步预定的氨基酸和/或肽的键合从N末端延伸得到的肽链，以获得所需的肽。

    Apparatus and method for conducting solid phase tests for mobile components in fluids
    50.
    发明公开
    Apparatus and method for conducting solid phase tests for mobile components in fluids 失效
    装置和用于在液体中进行可动部件的固相分析方法。

    公开(公告)号：EP0024174A1

    公开(公告)日：1981-02-25

    申请号：EP80302725.9

    申请日：1980-08-08

    申请人： Ventrex Laboratories Inc.

    发明人： Piasio, Roger Norman ,  Perry, David Allen ,  Nayak, Pangal Narayan

    IPC分类号： G01N33/54 ,  G01N33/56 ,  C12Q1/00

    CPC分类号： G01N33/54366 ,  Y10S436/81 ,  Y10S436/823

    摘要： Apparatus and method are provided for performing solid phase tests for mobile components in fluids, especially in vitro diagnostic tests. A solid phase device is used having a fixed component immobilized on its surface which is in contact with a fluid sample when inserted therein. The improvement involves utilizing a receptacle, into which the device is inserted, having the same fixed component immobilized on the inner surface of the receptacle. The test is performed by placing a fluid sample, having a mobile component reactive with the fixed component, into the receptacle and in contact with the insert for a period of time and measuring a change which is a function of the concentration of the mobile component.

    摘要翻译： 提供了用于在流体移动分量执行固相测定装置及方法，尤其是爱体外诊断测试。 固相装置，使用具有固定化在其表面的所有其与流体样品接触时插入其中的固定部件。 改进包括利用一个容器，在其中所述装置被插入时，其具有固定在插座的内表面上的相同的固定部件。 试验进行通过将流体样品，具有移动部件与固定组分反应，到插座和在与所述插入件用于在一段时间内接触并测量改变所有这一切是移动成分的浓度的函数。