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1134 条记录 (耗时 0.026 秒)

    NEW USE OF A FLUIDIC DEVICE
    53.
    发明授权
    NEW USE OF A FLUIDIC DEVICE 有权
    流体装置的新用途

    公开(公告)号：EP3019625B1

    公开(公告)日：2018-02-21

    申请号：EP14823735.7

    申请日：2014-07-10

    申请人： Gradientech AB

    发明人： KREUGER, Johan ,  THORSLUND, Sara ,  WU, Zhigang

    IPC分类号： C12Q1/18 ,  B01L3/00 ,  C12M1/12 ,  C12M3/06

    CPC分类号： C12Q1/18 ,  B01L3/5027 ,  C12M23/16 ,  C12M25/14

    摘要： A fluidic device (1) has a culture chamber (10) configured to house a 3D culture matrix (2) comprising a culture of microorganisms (6). A concentration gradient of a test substance is established over the 3D culture matrix (2) by providing respective fluid flows at different end portions (12, 14) of the culture chamber (10) and comprising different concentrations of the test substance. The response of the microorganisms (6) to the test substance is determined based on the position of a border zone (5) in the 3D culture matrix (2).

    RAPID DETERMINATION OF BACTERIAL SUSCEPTIBILITY TO AN ANTIBIOTIC AT THE POINT OF CARE
    54.
    发明公开
    RAPID DETERMINATION OF BACTERIAL SUSCEPTIBILITY TO AN ANTIBIOTIC AT THE POINT OF CARE 审中-公开

    公开(公告)号：EP3134538A4

    公开(公告)日：2017-11-15

    申请号：EP15783784

    申请日：2015-04-20

    申请人： POINT OF CARE DIAGNOSTICS LLC

    发明人： TURNER RONALD

    IPC分类号： C12Q1/04 ,  C12N1/20 ,  C12Q1/18

    CPC分类号： C12Q1/04 ,  C12Q1/66

    摘要： A method for determining whether bacteria in a sample obtained from a subject at a point of care in a clinical setting is susceptible to an antibiotic, within a time period associated with a point of care. The method includes measuring a bioluminescent indication from a first test sample based on released ATP to determine a characteristic associated with the bioluminescent indication and comparing the characteristic associated with the bioluminescent indication to a first threshold. The method includes determining whether a bacteria is present by comparing the difference between a characteristic associated with a first confirmatory bioluminescent signal and a characteristic associated with a second confirmatory bioluminescent signal to an confirmatory threshold. The method includes determining that bacteria is susceptible to an antibiotic by comparing a difference between a characteristic associated with a second bioluminescent signal and a characteristic associated with the first bioluminescent signal to a second threshold.

    IN VITRO ORAL BIOFILM MODELS OF INTERDENTAL SPACES AND USES
    57.
    发明公开
    IN VITRO ORAL BIOFILM MODELS OF INTERDENTAL SPACES AND USES 审中-公开
    体内口腔生物膜模型的INTERDENTAL空间和用途

    公开(公告)号：EP3207117A1

    公开(公告)日：2017-08-23

    申请号：EP14812024.9

    申请日：2014-11-11

    申请人： Colgate-Palmolive Company

    发明人： STETTLER, Hans ,  RUSSO, Amy

    IPC分类号： C12M1/12 ,  C12Q1/18

    CPC分类号： G01N33/56955 ,  C12M1/12 ,  C12Q1/18

    摘要： Disclosed is an in vitro oral biofilm model of interdental spaces including: a support, and a plurality of substratum pairs attached to the support, wherein an oral biofilm is capable of forming on the substratum pairs, wherein the substratum pairs each include a first member and a second member, and wherein the first member and the second member of each substratum pair are arranged to form a space interval between the first member and second member. Methods for assessing the formation of oral biofilms using the in vitro oral biofilm model and methods for testing agents, such as oral compositions, on biofilm reduction using the present model are also provided.

    摘要翻译： 公开了齿间空间的体外口腔生物膜模型，其包括：支撑件和附接到支撑件的多个基底对，其中口腔生物膜能够在基底对上形成，其中基底对各自包括第一构件和 第二构件，并且其中每个基底对的第一构件和第二构件被布置为在第一构件和第二构件之间形成空间间隔。 还提供了使用体外口腔生物膜模型评估口腔生物膜形成的方法和用于测试试剂例如口服组合物的方法，其使用本模型对生物膜减少。

    THERMOCHROMIC SENSING DEVICES, SYSTEMS, AND METHODS
    58.
    发明公开
    THERMOCHROMIC SENSING DEVICES, SYSTEMS, AND METHODS 审中-公开
    热敏感测装置，系统和方法

    公开(公告)号：EP3187593A1

    公开(公告)日：2017-07-05

    申请号：EP16204546.2

    申请日：2016-12-15

    申请人： Palo Alto Research Center, Incorporated

    发明人： RECHT, Michael I. ,  MARTINI, Joerg ,  KIESEL, Peter

    IPC分类号： C12Q1/04 ,  C12Q1/18 ,  G01N33/50

    CPC分类号： C12M41/12 ,  C12M23/12 ,  C12M23/20 ,  C12M31/00 ,  C12M41/18 ,  C12Q1/04 ,  C12Q1/18

    摘要： A test vessel includes one or more test locations configured to contain a medium suitable for culturing a live substance. A thermochromic material is thermally coupled to the one or more test locations. The thermochromic material is configured to exhibit a spectral shift in light emanating from the thermochromic material in response to an increase or decrease in energy conversion by the live substance that causes a change in temperature of the thermochromic material.

    摘要翻译： 测试容器包括一个或多个测试位置，所述测试位置被配置为包含适于培养活体物质的培养基。 热致变色材料热耦合到一个或多个测试位置。 响应于引起热致变色材料的温度变化的活物质的能量转换的增加或减少，热致变色材料被配置为展现从热致变色材料发出的光的光谱偏移。

    METHOD FOR THE MICROBIOLOGICAL DETERMINATION OF TRACES OF ANTIBIOTICS IN LOW VOLUME BIOLOGICAL SAMPLES
    59.
    发明公开
    METHOD FOR THE MICROBIOLOGICAL DETERMINATION OF TRACES OF ANTIBIOTICS IN LOW VOLUME BIOLOGICAL SAMPLES 审中-公开
    VERFAHREN ZUR MIKROBIOLOGISCHEN BESTIMMUNG VON SPUREN VON抗生素在BIOLOGISCHEN麻省理工学院GERINGEM VOLUMEN

    公开(公告)号：EP3085790A4

    公开(公告)日：2017-06-28

    申请号：EP14872796

    申请日：2014-12-11

    申请人： UNIV SANTIAGO CHILE

    发明人： SANHUEZA CHAVEZ LORETO ,  WILKENS ANWANDTER MARCELA ANDREA ,  LAURIDO FUENZALIDA CLAUDIO AURELIO

    IPC分类号： C12Q1/02 ,  C12Q1/18 ,  C12R1/445

    CPC分类号： C12Q1/18 ,  G01N2333/31

    摘要： A microbiological method for determining traces of antibiotics in low volume biological samples, which comprises determining the minimum inhibitory concentration (MIC) of an antibiotic selected from a group consisting of minocycline, ciprofloxacin, kanamycin, tetracycline and oxacillin against Staphylococcus aureus. The bacteria are grown overnight in Mueller-Hinton broth (MHB), said cultures subsequently being diluted to 0.5 McFarland standard (1.5 x 108 cells/mL). Said antibiotics are then added to 96-well plates to a final volume of 200 µL and incubated at 37°C for 18 hours. Absorbency is determined and the results are expressed as the percentage of inhibition relative to the control containing the biological sample, selected in turn from urine, blood, synovial fluid, eye aqueous humour or cerebrospinal fluid (CSF), and the bacterial culture. The curve representing the percentage of bacterial growth inhibition versus concentration of antibiotic is obtained, and fitted by "Four Parameter Logistic Curve (4PL)" analysis, yielding the antibiotic concentration values in the biological sample. The method allows to determine amounts of antibiotic in the biological sample even at ng/mL level.

    摘要翻译： 一种测定低体积生物样品中痕量抗生素的微生物学方法，其包括测定选自米诺环素，环丙沙星，卡那霉素，四环素和苯唑西林的抗生素对金黄色葡萄球菌的最小抑制浓度（MIC）。 细菌在Mueller-Hinton肉汤（MHB）中生长过夜，随后将所述培养物稀释至0.5McFarland标准（1.5×10 8个细胞/ mL）。 然后将所述抗生素加入到96孔板中至终体积为200μL，并在37℃下孵育18小时。 测定吸收性并将结果表示为相对于含有生物样品的对照的抑制百分比，其依次从尿，血液，滑液，眼房水或脑脊液（CSF）和细菌培养物中选择。 获得表示细菌生长抑制百分比对抗生素浓度的曲线，并且通过“四参数逻辑斯谛曲线（4PL）”分析进行拟合，产生生物样品中的抗生素浓度值。 该方法允许甚至在ng / mL水平下确定生物样品中抗生素的量。

    CARACTERISATION DE MICRO-ORGANISMES PAR MALDI-TOF
    60.
    发明公开
    CARACTERISATION DE MICRO-ORGANISMES PAR MALDI-TOF 审中-公开

    公开(公告)号：EP3174994A1

    公开(公告)日：2017-06-07

    申请号：EP15756680.3

    申请日：2015-07-29

    申请人： Biomérieux

    发明人： RAMJEET, Mahendrasingh ,  SURRE, Jérémy ,  FERULLO, Audrey

    IPC分类号： C12Q1/18 ,  G01N33/68 ,  C12Q1/04

    CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/18 ,  G01N1/36 ,  G01N33/6848

    摘要： The present invention relates to a method for preparing an area for characterizing an analysis plate for carrying out at least one characterization of a population of at least one microorganism in the presence of at least one antimicrobial agent via mass spectrometry according to the matrix-assisted laser desorption/ionization mass spectrometry technique known as MALDI. Said method includes the following consecutive steps: - a step that involves making use of an analysis plate intended for characterization via the MALDI technique and including at least one analysis area having an antimicrobial agent; - a step for depositing the population of at least one microorganism onto said analysis area in contact with the antimicrobial agent; - an incubation step that involves preserving the analysis plate during a sufficient period time and under conditions sufficient to allow interaction of the antimicrobial agent with the present microorganism; and - a step for depositing, on the analysis area, a matrix suited to the MALDI technique. The invention also relates to characterization and functionalization methods and to related analysis plates and uses.

    摘要翻译： 本发明涉及一种制备用于表征分析板的区域的方法，所述分析板用于在存在至少一种抗微生物剂的情况下通过质谱法根据基质辅助激光对至少一种微生物的群体进行至少一次表征 解吸/电离质谱技术称为MALDI。 所述方法包括以下连续步骤： - 涉及利用旨在用于通过MALDI技术表征的分析板并且包括至少一个具有抗微生物剂的分析区域的步骤; - 用于将至少一种微生物的群体沉积到与所述抗微生物剂接触的所述分析区域上的步骤; - 孵育步骤，其涉及在足够的时间段和足以允许抗微生物剂与本微生物相互作用的条件下保存分析板; 以及 - 在分析区域上存放适合于MALDI技术的矩阵的步骤。 本发明还涉及表征和功能化方法以及相关的分析板和用途。