公开(公告)号：EP3294751A1

公开(公告)日：2018-03-21

申请号：EP16722882

申请日：2016-05-13

申请人： GLAXO GROUP LTD

发明人： FINKA GARY BRIAN ,  HOARE MICHAEL ,  UDEN MARK ,  VOULGARIS IOANNIS

IPC分类号： C07K1/14

CPC分类号： C12N1/38 ,  C07K1/145 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/569 ,  C12P21/02

摘要： The present invention relates to a method of producing a recombinant protein in a host cell comprising adding Polyethyleneimine (PEI)during cell culture. Addition of PEI to the cell culture as a fermentation enhancer can result in reducing the viscosity of the cell culture, and/or increasing the extracellular concentration of the recombinant protein, and/or reducing the duration of cell culture to the point of harvest or protein recovery.

公开(公告)号：EP2702067B1

公开(公告)日：2018-02-21

申请号：EP12776139.3

申请日：2012-04-23

申请人： Billion King International Limited

发明人： Wong, Bing Lou ,  Kwok, Sui Yi

IPC分类号： C07K1/34 ,  C07K1/14 ,  C07K14/805 ,  C07K1/36

CPC分类号： C07K14/805

摘要： A method heat treatment of cross-linked hemoglobin solutions including polymeric hemoglobin is disclosed. The method involves contacting the hemoglobin solution with a high temperature short time heat treatment apparatus. The high temperature short time process thermally denatures unmodified tetrameric hemoglobin (hemoglobin dimer form), protein impurities (e.g. immunoglobin-G, serum albumin), bacteria, and viruses so that renal injury, vascular detrimental effects and other toxicity reactions can be avoided.

公开(公告)号：EP2480560B1

公开(公告)日：2018-02-21

申请号：EP10818191.8

申请日：2010-09-21

申请人： Medicago Inc.

发明人： VEZINA, Louis-Philippe ,  COUTURE, Manon ,  PAQUET, Dany ,  DARGIS, Michele ,  D'AOUST, Marc-Andre

IPC分类号： C07K1/14 ,  C07K1/34 ,  C07K14/415 ,  C12N5/04 ,  C12N15/00 ,  C12N15/82

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/517 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2760/16123 ,  C12N2760/16134

摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.

公开(公告)号：EP3281949A1

公开(公告)日：2018-02-14

申请号：EP16776503.1

申请日：2016-04-04

申请人： Spiber Inc. ,  Kojima Industries Corporation

发明人： ISHIDA Kana ,  YAMAMOTO Hironori ,  SUZUMURA Hiroaki ,  SEKIYAMA Kazuhide

IPC分类号： C07K14/435 ,  C07K1/14 ,  C07K14/00

CPC分类号： C08J3/097 ,  C07K1/14 ,  C07K14/00 ,  C07K14/435 ,  C08J2389/00 ,  C08L89/00 ,  C08L2203/16 ,  D01F1/10 ,  D01F4/02

摘要： A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5 × m × n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same.

摘要翻译： 本发明的极性溶剂溶液是极性溶剂溶液，其中含有聚氨基酸的溶质溶解在极性溶剂中。 将无机盐添加到溶液中，并且溶液中水分与无机盐的摩尔比为2.5×m×n或更小，其中m表示形成无机盐的阳离子的数量，n表示 阳离子。 本发明的制造方法是用于制造上述溶液的方法，其包括：改变溶液中无机盐的含水量和含量中的至少一个以调节溶液的粘度。 因此，本发明提供了一种极性溶剂溶液，其粘度可以容易地调节至所需的值，并因此当用作纺丝，膜等的涂料时能够稳定地纺丝和浇铸，以及其制备方法。

公开(公告)号：EP3116892A4

公开(公告)日：2017-11-22

申请号：EP14883670

申请日：2014-02-27

申请人： AGENCY SCIENCE TECH & RES

发明人： GAGNON PETER STANLEY

IPC分类号： B01D15/36 ,  B01D15/32 ,  C07K1/14 ,  C07K1/32

CPC分类号： C07K1/32 ,  B01D15/327 ,  B01D15/36 ,  B01D15/363 ,  B01D15/3828 ,  C07K1/145 ,  C07K1/34 ,  C07K1/36 ,  C07K16/00

摘要： A method of reducing the aggregate content in a preparation having a target protein includes contacting the preparation with an aryl anion to form a mixture and contacting the mixture with at least one electropositive solid to remove excess aryl anion.

公开(公告)号：EP3169697A4

公开(公告)日：2017-11-08

申请号：EP15822758

申请日：2015-07-13

申请人： GENNOVA BIOPHARMACEUTICALS LTD

发明人： RAGHUWANSHI ARJUN ,  SINGH SHRAWAN KUMAR ,  THAKER NIDHIBEN ,  SHANKAR SHAGUN ,  KARDILE PAVAN ,  SINGH SANJAY

IPC分类号： C07K1/14 ,  C07K14/535 ,  C12P21/00

CPC分类号： C07K14/535 ,  A61K9/0019 ,  A61K38/00 ,  A61K38/193 ,  A61K47/02 ,  A61K47/10 ,  A61K47/12 ,  A61K47/26 ,  A61K47/48215 ,  A61K47/60 ,  C07K1/02 ,  C07K1/14 ,  C07K1/18 ,  C07K1/34

摘要： The present invention provides a novel, scalable and industrially viable downstream process for purification of recombinant human G-CSF.

公开(公告)号：EP3228709A1

公开(公告)日：2017-10-11

申请号：EP15865944.1

申请日：2015-11-27

申请人： Wako Pure Chemical Industries, Ltd. ,  Osaka University

发明人： NISHIBU Takahiro ,  IMAWAKA Naoko ,  NARUSE Ken ,  HANAYAMA Rikinari

IPC分类号： C12N15/09 ,  C07K1/14 ,  C07K17/00 ,  G01N33/53

CPC分类号： G01N33/5076 ,  C07K1/14 ,  C07K14/70503 ,  C07K17/00 ,  C07K17/02 ,  C07K2319/40 ,  C12N7/02 ,  C12N15/09 ,  C12N2760/16151 ,  C12N2760/18551 ,  G01N33/53 ,  G01N33/5432 ,  G01N33/6842 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/149

摘要： An object of the present invention is to provide a carrier and a method for obtaining or removing extracellular membrane vesicle or virus present in a sample in good purity and in an intact state simply and conveniently, as well as and efficiently, or detecting them in high sensitivity.
The present invention relates to: "1. a carrier (a Tim carrier) on which a protein (a Tim protein), selected from a T-cell immunoglobulin and mucin domain-containing molecule-4 (a Tim-4) protein, a Tim-3 protein, and a Tim-1 protein, is bound; 2. a method for obtaining the extracellular membrane vesicle or the virus in the sample; 3. a method for removing the extracellular membrane vesicle or the virus in the sample; 4. a method for detecting the extracellular membrane vesicle or the virus in the sample; 5. a kit for capturing the extracellular membrane vesicle or the virus, comprising the Tim carrier; and 6. a kit for capturing the extracellular membrane vesicle or the virus, comprising a reagent containing the Tim protein and a reagent containing the carrier".

摘要翻译： 本发明的一个目的是提供一种载体和方法，用于简单且方便地并且有效地获得或除去存在于样品中的高纯度和完整状态的细胞外膜囊泡或病毒，或者在高 灵敏度。 本发明涉及：“1.载体（Tim载体），其上载有选自T细胞免疫球蛋白和含粘蛋白结构域的分子-4（Tim-4）蛋白质的蛋白质（Tim蛋白质）， Tim-3蛋白和Tim-1蛋白结合; 2.获得样品中细胞外膜囊泡或病毒的方法; 3.除去样品中细胞外膜囊泡或病毒的方法; 4。 5.检测样品中细胞外膜囊泡或病毒的方法; 5.用于捕获细胞外膜囊泡或病毒的试剂盒，其包含Tim载体;以及6.用于捕获细胞外膜囊泡或病毒的试剂盒， 包含含有Tim蛋白的试剂和含有载体的试剂“。

公开(公告)号：EP3228625A1

公开(公告)日：2017-10-11

申请号：EP15877635.1

申请日：2015-10-28

申请人： Osmun Biological Co., Ltd.

发明人： YANG, Anquan ,  WANG, Jing ,  ZHANG, Lihua

IPC分类号： C07K1/14 ,  C07K14/435

CPC分类号： C07K14/43504 ,  C07K1/12 ,  C07K1/145 ,  C07K1/36

摘要： The present invention involves a pearl protein preparation method and a water-soluble pearl protein and an acid-soluble pearl protein obtained by adopting this method. A pearl protein preparation method comprises the following steps: (1) grinding pearls into pearl powder; (2) performing ultra-high pressure homogenization to the nanoscale pearl powder using deionized water bath, proceeding with centrifugation at low temperature to obtain a water-soluble pearl extract a and sediments, and rinsing the sediments with ethanol solution; (3) dissolving the sediments obtained above in weak acid at certain temperature, and stirring at a low speed for reaction; performing centrifugation at low temperature, and obtaining a supernatant being an acid-soluble pearl extract b; (4) performing desalination to water-soluble pearl extract a and acid-soluble pearl extract b respectively; (5) freeze-drying the water-soluble pearl extract a and acid-soluble pearl extract b after desalination, and obtaining a water-soluble pearl protein and an acid-soluble pearl protein respectively. This invention uses nanoscale pearl powder, which provides raw materials for pearl skin care products and health care products.

摘要翻译： 本发明涉及一种珠蛋白制备方法，采用该方法得到的水溶性珍珠蛋白和酸溶性珍珠蛋白。 一种珍珠蛋白制备方法，包括以下步骤：（1）将珍珠研磨成珍珠粉; （2）用去离子水浴对纳米级珍珠粉进行超高压均质化处理，低温离心处理得到水溶性珍珠母提取物a和沉淀物，用乙醇溶液冲洗沉淀物; （3）将上述得到的沉淀物溶解于一定温度的弱酸中，低速搅拌反应; 在低温下离心分离，得到酸溶性珍珠提取物b的上清液; （4）分别对水溶性珍珠提取物a和酸溶性珍珠提取物b进行脱盐处理; （5）将脱水后的水溶性珍珠提取物a和酸溶性珍珠提取物b冷冻干燥，分别得到水溶性珠蛋白和酸溶性珠蛋白。 本发明采用纳米级珍珠粉，为珍珠护肤品和保健品提供原料。

公开(公告)号：EP2826383B1

公开(公告)日：2017-09-06

申请号：EP14185515.5

申请日：2009-07-10

申请人： Burcon Nutrascience (MB) Corp.

发明人： Schweizer, Martin ,  Green, Brent E. ,  Segall, Kevin I. ,  Logie, James

IPC分类号： A23J1/14 ,  A23J3/14 ,  A23L2/66 ,  C07K1/14 ,  C07K14/415 ,  A23L33/17

CPC分类号： A23J3/14 ,  A23J1/14 ,  A23V2002/00 ,  A23V2300/34 ,  A23V2300/38 ,  A23V2300/40

公开(公告)号：EP3200817A1

公开(公告)日：2017-08-09

申请号：EP15847588.9

申请日：2015-09-30

申请人： Antnor Limited

发明人： GALEA, Anthony ,  BROKHMAN, Irina

IPC分类号： A61K38/48 ,  A61K35/14 ,  A61K38/19 ,  A61P19/00 ,  A61P21/00 ,  C07K1/14 ,  C07K14/54 ,  C12N9/64

CPC分类号： A61K35/14 ,  A61K38/1841 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/2006 ,  C07K1/14 ,  C12N9/64 ,  A61K2300/00

摘要： Described are methods of producing an autologous composition useful for treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal. The method comprises preparing an anti-inflammatory/anti-catabolic component of the autologous composition comprising IL-1ra and TIMPs. An anti-inflammatory/anti-catabolic component is prepared comprising: collecting blood from the mammal; delivering the blood to a tube; incubating the blood at a temperature of from about 37° C. to about 39° C. for about 24 hours, preferably in the presence of sodium citrate; centrifuging the blood to separate the blood into a supernatant component and a cellular fraction; and collecting the supernatant component. The method further comprises preparing a regenerative component of the autologous composition comprising: collecting blood from the mammal; delivering the blood to a tube in the presence of about 4% citric acid; centrifuging the blood to separate a platelet-rich plasma component from a whole blood; collecting the platelet-rich plasma component; and mixing the supernatant component with the platelet-rich plasma component to provide the autologous composition. Also provided is a method of treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a subject with the autologous composition, an autologous composition for treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal and the use of the autologous composition for the treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal.

摘要翻译： 描述了生产用于治疗哺乳动物中的损伤和/或损伤的结缔组织，慢性肌腱炎，慢性肌肉撕裂和/或慢性退行性关节病症和皮肤炎性病症的自体组合物的方法。 该方法包括制备包含IL-1ra和TIMP的自体组合物的抗炎/抗分解代谢组分。 制备抗炎/抗分解代谢组分，包括：从哺乳动物采集血液; 将血液输送到管中; 优选在柠檬酸钠存在下，将血液在约37℃至约39℃的温度下孵育约24小时; 离心血液以将血液分离成上清液组分和细胞组分; 收集上清液组分。 该方法还包括制备自体组合物的再生组分的步骤，包括：从哺乳动物采集血液; 在约4％柠檬酸存在下将血液输送到管中; 离心血液以从全血中分离富血小板血浆成分; 收集富血小板血浆成分; 并且将上清液组分与富含血小板的血浆组分混合以提供自体组合物。 还提供了用自体组合物治疗受治疗者的受损和/或受伤结缔组织，慢性肌腱炎，慢性肌肉撕裂和/或慢性退行性关节病症和皮肤炎性病症的方法，用于治疗受损和/或受伤的自体组合物 结缔组织，慢性肌腱炎，慢性肌肉撕裂和/或慢性退行性关节病症和皮肤炎性病症，以及使用自体组合物治疗受损和/或受伤的结缔组织，慢性肌腱炎，慢性肌肉撕裂和/ 或哺乳动物中的慢性退行性关节病症和皮肤炎性病症。