公开(公告)号：EP1231259A3

公开(公告)日：2003-11-19

申请号：EP02004895.5

申请日：1999-07-22

申请人： Qinetiq Limited

发明人： Canham, Leigh Trevor

IPC分类号： C12M3/00 ,  C12N15/89 ,  C12N15/87

CPC分类号： C12N15/87 ,  A61K9/0021 ,  A61M37/0015 ,  A61M2037/0046 ,  A61M2037/0053 ,  C12M35/00 ,  C12N15/89 ,  C12N15/895

摘要： The present invention relates to the use of resorbable and/or bioactive silicon in the delivery of substances to cells. The silicon may be formed into biolistic bullets for penetration of the cell. The control of the pore size and porosity of porous silicon allows tuning of the bioactivity and/or resorbablitiy of the silicon. The present invention also relates to the advantages over known methods of delivering materials into cells.

摘要翻译： 本发明涉及可再吸收和/或生物活性硅在向细胞递送物质中的用途。 硅可以形成用于细胞穿透的生物弹子弹。 多孔硅的孔径和孔隙度的控制允许调节硅的生物活性和/或再吸收性。 本发明还涉及到将材料输送到电池中的已知方法的优点。

公开(公告)号：EP1051244A4

公开(公告)日：2003-06-11

申请号：EP99901307

申请日：1999-01-06

申请人： TOMALIA DONALD A

发明人： TOMALIA DONALD A ,  BALOGH LAJOS

IPC分类号： C12N15/09 ,  B01J13/02 ,  C08G83/00 ,  C12M1/00 ,  C12N5/10 ,  C12N15/89 ,  C08F299/00 ,  C08G63/48 ,  G01N33/545

CPC分类号： C08G83/003 ,  B01J13/02 ,  C12N15/895 ,  Y10S977/914 ,  Y10S977/916

摘要： A gene transfection particle includes a polymer, a support particle conjugated with the dendritic polymer, and genetic material conjugated with the dendritic polymer. The gene transfection particles are highly efficient and are capable of delivering higher quantities of genetic materials to cells, with reduced cell damage. A gene transfection method involves bombarding cells with conjugates of polymers and genetic material, with or without a support particle.

公开(公告)号：EP1245149A1

公开(公告)日：2002-10-02

申请号：EP01107969.6

申请日：2001-03-29

申请人： greenovation Biotech GmbH

发明人： Bock, Ralph ,  Carrer, Helaine

IPC分类号： A01H4/00 ,  A01H5/08 ,  A01H1/00 ,  C12N15/89 ,  C12N15/82

CPC分类号： C12N15/8214

摘要： The present invention provides methods for obtaining stably transformed transplastomic plant cell material belonging to the genus Lycopersicon (tomato) and having the potential to regenerate into mature fertile plants. The method generally comprises (a) transforming plastids of said plant cell material with a DNA molecule carrying an expression cassette encoding at least one protein of interest, and a target sequence enabling homologous recombination; (b) selecting plant cell material until substantially all plastids have been transformed with said DNA molecule, thereby obtaining said stably transformed transplastomic plant cell material; and, optionally (c) regenerating said transplastomic plant cell material into mature fertile plants. Furthermore, the present invention provides stably transformed transplastomic Lycopersicon plant cells, seeds, tissues and organs having the potential to regenerate into mature fertile plants, as well as mature fertile plants, obtainable or obtained by carrying out the methods according to the invention.

摘要翻译： 本发明提供用于获得属于番茄属（番茄）的稳定转化的转基因植物细胞材料的方法，并且具有再生成成熟可育植物的潜力。 该方法通常包括（a）用携带编码至少一种目的蛋白质的表达盒的DNA分子和能够进行同源重组的靶序列转化所述植物细胞材料的质体; （b）选择植物细胞材料，直到基本上所有的质体已经用所述DNA分子转化，从而获得所述稳定转化的转基因植物细胞材料; 和（c）将所述转基因植物细胞材料再生成成熟的可育植物。 此外，本发明提供了通过实施本发明的方法可获得或获得的具有可再生成成熟可育植物以及成熟可育植物的稳定转化的转基因番茄植物细胞，种子，组织和器官。

公开(公告)号：EP0941318B9

公开(公告)日：2001-12-19

申请号：EP97949949.8

申请日：1997-11-13

申请人： Soft Gene GmbH

发明人： WITTIG, Burghardt ,  JUNGHANS, Claas

IPC分类号： C12N15/11 ,  C12N15/89 ,  A61K48/00

CPC分类号： C12N15/87 ,  A61K48/00 ,  Y10S977/916

摘要： The invention concerns an expressible nucleic acid construct which contains only the sequence information necessary for expressing a gene for RNA or protein synthesis. Expression constructs of this type can be used in gene therapy and genetic vaccination and avoid many of the risks associated with constructs today. The invention further concerns the possibility of improving the conveying of the construct into cells or tissue by covalent linkage of the construct, for example to particles or peptides.

摘要翻译： 本发明涉及可表达的核酸构建体，其仅含有表达RNA或蛋白质合成基因所必需的序列信息。 这种表达构建体可用于基因治疗和基因疫苗接种，并避免今天与构建体相关的许多风险。 本发明进一步涉及通过构建体例如颗粒或肽的共价连接改善构建体向细胞或组织的输送的可能性。

公开(公告)号：EP1047792A1

公开(公告)日：2000-11-02

申请号：EP99924219.1

申请日：1999-05-13

申请人： CEDARS-SINAI MEDICAL CENTER ,  IMPERIAL COLLEGE OF SCIENCE,TECHNOLOGY & MEDICINE

发明人： READHEAD, Carol, W. ,  WINSTON, Robert

IPC分类号： C12N15/89 ,  C12N15/86 ,  C12N15/88 ,  C12N5/06 ,  A01K67/027 ,  A61K48/00

CPC分类号： A01K67/0275 ,  A01K67/027 ,  A01K2217/05 ,  A61K35/12 ,  A61K48/00 ,  C12N5/061 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2799/021 ,  C12N2799/022 ,  C12N2799/027

摘要： A composition for in vitro and in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A method of genetically altering a vertebrate male germ cell in vivo employs a lentiviral-derived vector. A method of substantially depopulating a vertebrate testis employs a combination of a dose of an alkylating agent, such as busulfan, chlorambucil, cyclophosphamide, melphalan, or ethyl ethanesulfonic acid, and a dose of gamma radiation. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

公开(公告)号：EP0992577A1

公开(公告)日：2000-04-12

申请号：EP98110294.0

申请日：1998-06-05

申请人： Lummel, Wolfgang

发明人： Knoblauch, Michael

IPC分类号： C12M3/00 ,  B01L3/02 ,  C12N15/89

CPC分类号： C12N15/89 ,  C12M35/00

摘要： Die Erfindung betrifft ein Mikroinjektionsverfahren zum Einbringen eines Injektionsstoffes, insbesondere genetisches Material, in Prokaryoten- und Eukaryotenzellen, sowie Zellkompartimente von letzteren (Plastiden, Zellkerne). Die offensichtlichsten Nachteile im Stand der Technik sind mit der Verletzung der Zelle durch die Glaspipette verbunden. Hier schafft die Erfindung dadurch Abhilfe, daß man die Nanopipette (10), welche einen Aussendurchmesser von 0,05 - 0,2 µm, einen Innendurchmesser von 0,1 - 1,5 mm und einen Spitzendurchmesser von 0,025 bis 0,3 µm besitzt, mit dem mit dem
Injektionsstoff (1) und einem durch Wärme expandierbaren Stoff oder -gemisch füllt und die Kapillare der Nanopipette (10) anschließend mit einem Klebemittel verschließt, die Pipettenspitze mit Hilfe eines Mikroskopes und eines Mikromanipulators in die gewünschte Plastide, das Bakterium oder das Zellkompartiment/Zellkern einsticht, und die Nanopipette mittels einer regelbaren Heizvorrichtung (12) erwärmt, bis der Injektionsstoff mit einer Ausströmgeschwindigkeit im Bereich bis zu 1 Femtoliter pro Sekunde an der Pipettenspitze aus- und in die Plastide, das Bakterium oder das Zellkompartiment/den Zellkern, deren Durchmesser im Bereich von 1 - 20 µm liegt, eintritt.
Die Erfindung ist ebenfalls die entsprechend gefüllte Nanopipette selbst, die von einer regelbaren Heizvorrichtung zur thermogesteuerten Injektion erwärmbar ist.

摘要翻译： 用于引入的材料显微注射法（I），特别是爱外源遗传物质，到原核或真核细胞和细胞区室（质体和核），使用纳米吸管和压力，是新的。 用于引入的材料显微注射法（I），特别是爱外源遗传物质，到原核或真核细胞和细胞区室（质体和核），使用纳米吸管和压力，是新的。 纳米移液器具有至0.5外径 - 2mm至0.1内径 - 1.5毫米和0.025一个点测量 - 0.3微米，并且填充有具有温暖膨胀材料混合的注射材料。 纳米移液管的毛细管然后用粘接剂，插入到质体，细菌，细胞用显微镜和微操纵器和纳米吸管隔室或细胞核中的移液管尖端加温利用加热装置关闭。 该注射材料被传递到质体，细菌，细胞室或细胞核（其中有一个直径1 - 20亩升）与约每秒1亩升的速度。 因此独立claimsoft包括用于纳米移液器用于执行（I）。

公开(公告)号：EP0960933A1

公开(公告)日：1999-12-01

申请号：EP99109415.2

申请日：1999-05-11

申请人： Micronas Intermetall GmbH

发明人： Baumann, Werner, Dr. ,  Ehret, Ralf, Dr. ,  Lehman, Mirko, Dipl.-Phys. ,  Igel, Günter, Dipl.-Ing. ,  Gahle, Jürgen, Dr. Ing. ,  Wolf, Bernhard, Prof. Dr. ,  Sieben, Ulrich, Dr. Dipl-Phys. ,  Freund, Ingo, Dipl.-Ing. ,  Brischwein, Martin, Dr.

IPC分类号： C12M3/00 ,  C12N15/89

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

摘要： Die Erfindung betrifft ein Verfahren zur Messung einer Zustandsgröße einer biologischen Zelle (3), die in einem Nährmedium (2) adhärent an einem Auflagebereich (5) angelagert wird. Innerhalb des Auflagebereichs (5) der Zelle (3) wird mit Abstand von dessen Rand eine Öffnung in die Zellmembran der Zelle (3) eingebracht. Dabei dichtet der die Öffnung umgrenzende, an dem Auflagebereich (5) anhaftende Rand der Zellmembran die im Inneren der Zelle (3) befindliche Zellflüssigkeit gegen das Nährmedium (2) ab. Durch die Öffnung hindurch wird die Zustandsgröße (2) gemessen. Die Erfindung betrifft ferner eine Vorrichtung zur Durchführung des Verfahrens.

摘要翻译： 开口被引入到电池安装件（5）的区域内的电池（3）膜中并且与支撑边缘隔开。包括用于适当设备的独立权利要求包括电池安装例如电池。 适当的仪器，电极和/或辐射能源。

公开(公告)号：EP0587798A4

公开(公告)日：1995-04-19

申请号：EP92914194

申请日：1992-06-08

申请人： DOWELANCO

发明人： WALSH TERENCE A ,  OWENS MERLO PATRICIA ANN ,  STRICKLAND JAMES A ,  ORR GREGORY L ,  MERLO DONALD J ,  WALDRON CLIVE

IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N65/00 ,  C07K14/00 ,  C07K14/415 ,  C07K14/81 ,  C12N1/21 ,  C12N5/10 ,  C12N9/18 ,  C12N15/09 ,  C12N15/82 ,  C12N15/89 ,  C12P21/02 ,  C12N15/11 ,  A01H1/06 ,  C12N9/50 ,  C12N15/29

CPC分类号： C12N15/8286 ,  A01N63/00 ,  C07K14/415 ,  C07K14/8139 ,  C12N9/18 ,  C12N15/89 ,  Y02A40/162

摘要： The present invention provides a composition and method of using certain cysteine protease inhibitors to protect plants otherwise susceptible to insect infestation by one or more of Mexican bean beetle, red flour beetle, confused flour beetle, cowpea beetle, boll weevil, Colorado potato beetle, three-lined potato beetle, rice weevil, maize weevil, granary weevil, lesser grain borer, flea beetles, Egyptian alfalfa weevil, bean weevil, yellow mealworm, asparagus beetle and squash bug.

公开(公告)号：EP4219730A1

公开(公告)日：2023-08-02

申请号：EP23157476.5

申请日：2016-01-27

申请人： INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES

发明人： GAO, Caixia ,  ZHANG, Yi ,  WU, Zhongyi ,  ZHANG, Kang

IPC分类号： C12N15/89 ,  C12N15/84

摘要： The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant. In the present invention, tissue culture is omitted by transient expression of the sequence-specific nuclease; mutation is obtained at whole plant level; the method is independent of the genotype and recipient, and thus can be applied to various varieties of various species; T1 mutants can be obtained directly and the mutation can be stable inherited; more importantly, the mutant plant as obtained is free of exogenous genes, and thus have higher bio-safety.

公开(公告)号：EP3252162B1

公开(公告)日：2023-03-01

申请号：EP16742765.7

申请日：2016-01-27

发明人： GAO, Caixia ,  ZHANG, Yi ,  WU, Zhongyi ,  ZHANG, Kang

IPC分类号： C12N15/89 ,  C12N15/84