ATTENUATED AND ADAPTED STRAIN OF PSEUDOMONAS FOR DELIVERING ANTIGENS
    51.
    发明公开
    ATTENUATED AND ADAPTED STRAIN OF PSEUDOMONAS FOR DELIVERING ANTIGENS 审中-公开
    ABGESCHWÄCHTEUND ANGEPASSTE PSEUDOMONAS-STÄMMEZUR FREISETZUNG VON ANTIGENEN

    公开(公告)号:EP2791318A1

    公开(公告)日:2014-10-22

    申请号:EP12805669.4

    申请日:2012-12-12

    IPC分类号: C12N1/20 C12R1/38 A61K39/00

    摘要: The invention is related to a process for obtaining an adapted strain of Pseudomonas, comprising the following steps: €¢ deleting the genes ExoS ExoT, aroA and lasI in an initial Pseudomonas strain cultivated in a LB medium, €¢ progressively cultivating this strain in a chemically defined medium based on a glucose minimal medium supplemented with magnesium and calcium, wherein the adapted strain presents the same toxicity and secretion capacities than the initial strain, and its doubling time when cultivated in said chemically defined medium is less than 60 minutes. The present invention is also related to the adapted strain such as obtained,in particular wherein the adapted strain is furthermore treated to become 'killed but metabolically active'.

    摘要翻译: 本发明涉及一种获得拟南芥假单胞菌菌株的方法,其包括以下步骤:删除在LB培养基中培养的初始假单胞菌属菌株中的ExoS ExoT,aroA和lasI基因,并逐渐培养该菌株 基于补充有镁和钙的葡萄糖基本培养基的化学上确定的培养基,其中所述适应菌株具有与初始菌株相同的毒性和分泌能力,并且其在所述化学确定的培养基中培养的倍增时间小于60分钟。 本发明还涉及适应的菌株,例如获得的,特别是其中将适应菌株进一步处理成“杀死但代谢活性”。

    COMPLEX CRYSTALLINE SUGAR COMPRISING D-PSICOSE AND D-ALLOSE AND PROCESS FOR PRODUCTION OF THE SAME
    54.
    发明公开
    COMPLEX CRYSTALLINE SUGAR COMPRISING D-PSICOSE AND D-ALLOSE AND PROCESS FOR PRODUCTION OF THE SAME 有权
    COMPLEX,D-阿洛酮糖和D-阿洛糖含有结晶糖和方法及其生产

    公开(公告)号:EP1860195A4

    公开(公告)日:2011-10-19

    申请号:EP06715211

    申请日:2006-03-03

    IPC分类号: C12P19/02 C12P19/24 C12R1/38

    CPC分类号: C12P19/02 C12P19/24

    摘要: Disclosed is a process for producing a crystalline sugar comprising D-psicose and D-allose. Also disclosed is a process for producing the sugar. A complex crystalline sugar comprising D-psicose and D-allose. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.