-
公开(公告)号：EP1399575B1
公开(公告)日：2006-07-05
申请号：EP01992495.0
申请日：2001-10-31
发明人： ECONOMIDES, Aris, N. , MURPHY, Andrew, J. , VALENZUELA, David, M. , FRENDEWEY, David , YANCOPOULOS, George, D.
IPC分类号： C12N15/90
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , C12N15/907
摘要： A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous genes(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
-
公开(公告)号：EP1392859B1
公开(公告)日：2006-05-10
申请号：EP02713420.4
申请日：2002-01-16
IPC分类号： C12N5/06 , C12N5/08 , G01N33/50 , G01N33/569 , G01N33/68 , C07K16/28 , C07K14/315 , C07K14/52
CPC分类号： C40B30/04 , A01K2217/05 , A01K2267/01 , C07K14/315 , C07K14/52 , C07K16/2863 , C07K2317/77 , C07K2319/00 , C12N15/1051 , G01N33/56966 , G01N33/6845 , G01N2500/10
摘要： A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.
-
公开(公告)号：EP1572967A2
公开(公告)日：2005-09-14
申请号：EP03779216.5
申请日：2003-10-24
IPC分类号： C12N1/00
CPC分类号： C07H21/04 , A61K38/00 , C07K14/715 , C07K14/7155 , C07K16/244 , C07K2317/622 , C07K2317/73 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/32
摘要： The present invention provides a fusion polypeptide capable of binding interleukin -1 (IL-1) to form a nonfunctional complex. It also provides a nucleic acid sequence encoding the fusion polypeptide and methods of making and uses for the fusion polypeptide.
-
公开(公告)号：EP1392859A2
公开(公告)日：2004-03-03
申请号：EP02713420.4
申请日：2002-01-16
IPC分类号： C12Q1/68
CPC分类号： C40B30/04 , A01K2217/05 , A01K2267/01 , C07K14/315 , C07K14/52 , C07K16/2863 , C07K2317/77 , C07K2319/00 , C12N15/1051 , G01N33/56966 , G01N33/6845 , G01N2500/10
摘要： A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.
-
公开(公告)号：EP1392111A1
公开(公告)日：2004-03-03
申请号：EP02741823.5
申请日：2002-06-05
CPC分类号： C12N15/8509 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0393 , C12N15/907 , C12N2800/30 , C12N2830/00
摘要： The present invention is a method of targeting promoter-less selection cassettes into transcriptionally active loci. In particular, the invention is a method for targeting promoter-less selection cassettes into transcriptionally active loci in stem cells or other eukaryotic cells with much greater efficiency than previously observed with other methods, thus reducing or eliminating the need to screen for targeted cells. The invention also encompasses the DNA targeting vectors, the targeted cells, as well as non-human organisms, especially mice, created from the targeted cells.
摘要翻译： 本发明是将无启动子选择盒靶向转录活性基因座的方法。 特别地，本发明是一种以比先前用其他方法观察到的效率高得多的效率将无启动子选择盒靶向至干细胞或其他真核细胞中的转录活性基因座的方法，因此减少或消除筛选靶细胞的需要。 本发明还包括由靶向细胞产生的DNA靶向载体，靶向细胞以及非人生物体，尤其是小鼠。
-
66.发明授权MODULATION OF VASCULAR PERMEABILITY BY MEAN OF TIE2 RECEPTOR ACTIVATORS 有权调节装置GEFÄSSEPERMEABILITÄTTie2受体激活剂
公开(公告)号：EP1165115B1
公开(公告)日：2003-05-28
申请号：EP00916545.7
申请日：2000-03-20
IPC分类号： A61K38/17 , A61K38/18 , A61K39/395 , A61P9/00
CPC分类号： A61K38/1891 , A61K2039/505 , C07K16/22
摘要： The present invention provides for a method of decreasing or inhibiting vascular permeability and/or plasma leakage in a mammal comprising administering to the mammal a TIE2 receptor activator. The invention also provides for a method of decreasing or inhibiting vascular permeability and/or plasma leakage in a mammal comprising administering to the mammal an Ang-2 inactivator such as an anti-Ang-2 neutralizing antibody.
-
公开(公告)号：EP0533846B1
公开(公告)日：2000-08-09
申请号：EP91913637.4
申请日：1991-06-03
IPC分类号： C12N15/12 , C12N5/10 , C12P21/08 , G01N33/53 , G01N33/569 , A01K67/027 , G01N33/68 , A61K48/00 , C12Q1/68 , C07K14/00 , A61K38/00
CPC分类号： C07K14/705 , A61K38/00 , C07K2319/00 , C07K2319/41 , C07K2319/75 , C12Q1/6883 , C12Q2600/158
摘要： The present invention relates to the ciliary neurotrophic factor (CNTF) receptor, and provides for CNTF receptor nucleic acid and amino acid sequences. It also relates to (i) assay systems for detecting CNTF activity; (ii) experimental model systems for studying the physiologic role of CNTF; (iii) diagnostic techniques for identifying CNTF-related neurologic conditions; (iv) therapeutic techniques for the treatment of CNTF-related neurologic and muscular conditions, and (v) methods for identifying molecules homologuous to CNTF and CNTFR.
-
公开(公告)号：EP0656062B1
公开(公告)日：2000-03-08
申请号：EP93921339.3
申请日：1993-09-02
发明人： VALENZUELA, David, M. , HARLAND, Richard, M. , IP, Nancy, Y. , SMITH, William, C. , YANCOPOULOS, George, D. , CUDNY, Henryk , LAMB, Teresa , KNECHT, Anne
IPC分类号： C12N15/16 , C07K14/00 , C12N1/21 , C12N5/10 , C12P21/02 , A61K38/08 , C12N5/02 , C12P21/08 , C12N5/12
CPC分类号： C07K16/22 , A61K38/00 , A61K39/00 , C07K14/475
摘要： Novel dorsal growth inducing factors, complexes including the factors, and DNA or RNA coding sequences for the factors are described.
-
公开(公告)号：EP0971730A2
公开(公告)日：2000-01-19
申请号：EP98917988.2
申请日：1998-04-03
CPC分类号： G01N33/566 , G01N33/6887 , G01N2333/475 , G01N2333/78 , G01N2333/912
摘要： The present invention provides for use of a molecule capable of competing with collagen for binding to the extracellular domain of a Tyro-10 (DDR-2) or NEP (DDR-1) receptor in the manufacture of a medicament for use in a therapeutic method which comprises modulating or blocking the binding of collagen to Tyro-10 (DDR-2) or NEP (DDR-1) extracellular domain-expressing cells. The invention also provides for a screen for a polypeptide ligand that can bind to the Tyro-10 (DDR-2) or NEP (DDR-1) receptor and that can promote a differential function and/or influence the phenotype, such as growth and/or proliferation, of cells that bear the receptor. The present invention also provides a method of screening for a molecule capable of competing with collagen for binding to the extracellular domain of a Tyro-10 (DDR-2) or NEP (DDR-1) receptor comprising contacting a sample suspected of containing the molecule with the extracellular domain of a Tyro-10 (DDR-2) or NEP (DDR-1) receptor in the presence of collagen under conditions in which the collagen is capable of binding to the extracellular domain and detecting binding of the molecule to the Tyro-10 (DDR-2) or NEP (DDR-1) receptor extracellular domain.
-
公开(公告)号：EP0859840A1
公开(公告)日：1998-08-26
申请号：EP96937006.0
申请日：1996-10-25
CPC分类号： C07K14/4702
摘要： A novel ligand (Efl-6) that binds the Elk subfamily of Eph receptors is identified, and methods for making the soluble Elf-6 ligand in biologically active form is described. A cDNA clone encoding this novel protein enables production of the recombinant protein, which is useful to support neuronal and other Eph receptor-bearing cell populations.
-
-
-
-
-
-
-
-
-
搜索结果
73 条记录 (耗时 0.031 秒)
