利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

396 条记录 (耗时 0.069 秒)

    TREATMENT OF BACTERIAL DISEASES WITH GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR
    66.
    发明公开
    TREATMENT OF BACTERIAL DISEASES WITH GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR 失效
    粒细胞 - 巨噬细胞集落引起的疾病的利用系数用于细菌治疗的药物中的用途。

    公开(公告)号：EP0317568A1

    公开(公告)日：1989-05-31

    申请号：EP87905063.0

    申请日：1987-07-20

    申请人： IMMUNEX CORPORATION

    发明人： GRABSTEIN, Kenneth, Howard ,  MORRISSEY, Philip, J.

    IPC分类号： A61K38 ,  A61P31 ,  C07K14

    CPC分类号： C07K14/535 ,  A61K38/00 ,  Y10S930/145

    摘要： Des sujets souffrant de maladies infectieuses sont traitées par administration directe de quantités thérapeutiquement efficaces d'un facteur de stimulation de colonies de granulocytes-macrophages utilisées par lui-même ou conjointement avec un antibiotique ou un sulfonamide ou d'autres agents thérapeutiques immunologiquement efficaces. Le facteur de stimulation de colonies de granulocytes-macrophages homogènes utilisé dans le traitement de maladies bactériennes est préparé par des techniques d'ADN recombinant puis est purifié jusqu'à son homogénéité par chromatographie liquide de haute performance en phase inverse de sorte qu'il peut être administré sans danger à des sujets souffrant de maladies infectieuses.

    Detection of inflammation and novel antibodies therefor
    67.
    发明公开
    Detection of inflammation and novel antibodies therefor 失效
    Feststellung einerEntzündungundAntikörperdazu。

    公开(公告)号：EP0245052A2

    公开(公告)日：1987-11-11

    申请号：EP87303946.5

    申请日：1987-05-01

    申请人： IMMUNEX CORPORATION

    发明人： Conlon, Paul Jerome III ,  Prickett, Kathryn Susan

    IPC分类号： G01N33/564 ,  G01N33/68 ,  G01N33/577 ,  A61K39/395

    CPC分类号： G01N33/6869 ,  C07K16/245 ,  G01N33/564 ,  G01N2800/52

    摘要： An assay, test kit and novel antibodies for use therein are provided for detecting the presence of inflammation caused, for instance, by infectious disease or immune dysfunction, measures the level of IL-1 in body fluid, such as serum. Various assay techniques may be employed including direct assays, competitive assays and double determinant assays. The assay and test kit employs novels polyclonal or monoclonal antibodies specific for unique antigenic sites on the IL-1 protein or IL-1 peptide.

    摘要翻译： 提供用于其中的测定，测试试剂盒和新型抗体用于检测例如由感染性疾病或免疫功能障碍引起的炎症的存在，测量体液（例如血清）中IL-1的水平。 可以使用各种测定技术，包括直接测定，竞争性测定和双重决定簇测定。 测定和测试试剂盒使用对IL-1蛋白或IL-1肽独特抗原位点特异的多克隆或单克隆抗体。

    Interleukin-2 receptor and DNA encoded therefor and their preparation
    69.
    发明公开
    Interleukin-2 receptor and DNA encoded therefor and their preparation 失效
    白细胞介素-2受体激动剂，dafürkodierende DNS und deren Herstellung。

    公开(公告)号：EP0162699A2

    公开(公告)日：1985-11-27

    申请号：EP85303575.6

    申请日：1985-05-21

    申请人： IMMUNEX CORPORATION

    发明人： Cerretti, Douglas P. ,  Cosman, David J. ,  Dower, Steven K. ,  March, Carl J. ,  Urdal, David L. ,  Larsen, Alf D.

    IPC分类号： C12N15/12 ,  C12N15/85 ,  C12N5/10 ,  C12P21/02

    CPC分类号： C07K14/7155 ,  C07K16/2866

    摘要： Interleukin-2 receptor derived from normal and malignant cells has been purified by use of various techniques including affinity chromatography in conjunction with a monoclonal antibody directed to the receptor. The purification process also includes reversed phased high performance liquid chromatography. By these techniques, interleukin-2 receptor has been purified to homogeneity. The high purification of the interleukin-2 receptor has made possible the sequencing of the amino acid residues at the N-terminal of this protein molecule. Double-stranded cDNA is prepared from polyadenylated RNA extracted from cell lines or other sources known to produce IL-2 receptor. The cDNA is inserted within a plasmid vector and then the recombinant plasmid employed to transform an appropriate host. Transformed hosts are identified and grouped into pools. Plasmid DNA prepared from these pools is hybridized with a labeled synthetic oligonucleotide probe corresponding to a portion of the amino acid sequence of the purified IL-2 receptor. The cDNA clone isolated with the probe is characterized by restriction enzyme mapping and sequenced by chain-termination method. The particular DNA clone that actually contains the gene coding for the functional IL-2 receptor is identified by expressing the clones in COS-7 monkey kidney cells and assaying for the expressed IL-2 receptor by its ability to bind IL-2 or a monoclonal antibody directed against the IL-2 receptor.

    摘要翻译： 来自正常和恶性细胞的白细胞介素-2受体已经通过使用各种技术进行纯化，包括亲和层析与针对受体的单克隆抗体。 纯化方法还包括反相高效液相色谱法。 通过这些技术，白细胞介素-2受体已经被纯化到同质性。 白细胞介素-2受体的高度纯化使得可以对该蛋白质分子的N-末端的氨基酸残基进行测序。 从从已知产生IL-2受体的细胞系或其他来源提取的聚腺苷酸化RNA制备双链cDNA。 将cDNA插入质粒载体中，然后将重组质粒用于转化合适的宿主。 已识别转换的主机并将其分组到池中。 从这些池制备的质粒DNA与对应于纯化的IL-2受体的氨基酸序列的一部分的标记的合成寡核苷酸探针杂交。 用探针分离的cDNA克隆的特征在于限制酶作图并通过链终止法进行测序。 通过在COS-7猴肾细胞中表达克隆并通过其结合IL-2或单克隆抗体的能力测定表达的IL-2受体来鉴定实际含有编码功能性IL-2受体的基因的特定DNA克隆 针对IL-2受体的抗体。

    Serum-free cell culture medium and process for making same
    70.
    发明公开
    Serum-free cell culture medium and process for making same 失效
    无血清细胞培养基及制备方法

    公开(公告)号：EP0112174A3

    公开(公告)日：1985-05-15

    申请号：EP83307637

    申请日：1983-12-15

    申请人： IMMUNEX CORPORATION

    发明人： Baker, Paul E.

    IPC分类号： C12N05/00

    CPC分类号： C12N5/0037 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/84

    摘要： A substitute for serum in a cell culture medium comprises fetuin, transferrin, and phosphatidylcholine having the general formula:
    wherein:
    R, is either a saturated fatty acid selected from the group consisting essentially of lauric acid, myristic acid, palmitic acid and stearic acid; or an unsaturated fatty acid selected from the group consisting essentially of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and R 2 is conversely either an unsaturated fatty acid selected from the group consisting essentially of oleic acid, linoleic acid, linolenic acid and arachidonic acid; or a saturated fatty acid selected from the group consisting essentially of lauric acid, myristic acid, palmitic acid and stearic acid. A serum-free medium comprises such substitute together with inorganic salts, amino acids carbohydrates, buffering agents, vitamins and compounds to simulate the natural cell environment. It is capable of growing a wide range of suspension and monolayer cells.