公开(公告)号：EP1945663A1

公开(公告)日：2008-07-23

申请号：EP06779123.6

申请日：2006-08-11

申请人： Cancer Research Technology Limited

发明人： HASSAN, Andrew, Bassim ,  ZACCHEO, Oliver ,  PRINCE, Stuart

IPC分类号： C07K14/71 ,  A61K38/17 ,  C07K19/00 ,  C12N15/12 ,  C12N15/63

CPC分类号： C07K14/475 ,  A61K35/36 ,  A61K35/66 ,  A61K38/18 ,  A61K48/005 ,  C07K14/71 ,  C07K16/461 ,  C07K2319/20 ,  C07K2319/30 ,  C12N15/11 ,  C12N15/63 ,  C12N15/815 ,  C12N15/85

摘要： This invention relates to modified IGF-II binding domains of the Insulin-like Growth Factor 2 Receptor (IGF2R) which have enhanced binding affinity for IGF-II relative to the wild type IGF-II binding domain. Suitable IGF-II binding domains may be modified, for example, by substituting residue E1544 for a non-acidic residue. These modified domains may be useful in the sequestration of Insulin-like Growth Factor II (IGF-II), for example, in the treatment of cancer.

公开(公告)号：EP1919958A1

公开(公告)日：2008-05-14

申请号：EP06778357.1

申请日：2006-08-25

申请人： Laboratoires Serono SA

发明人： DUPRAZ, Philippe ,  KOBR, Michel

IPC分类号： C07K19/00 ,  C12N15/62 ,  C12N5/10 ,  C12N15/63 ,  G01N33/50

CPC分类号： C12N9/1205 ,  C07K2319/20 ,  C07K2319/60 ,  C12N9/1029 ,  C12N15/64 ,  G01N33/5023

摘要： This invention refers to industrial production of proteins. More particularly, the invention refers to a fusion protein as a novel chimeric selection marker comprising a peptide conferring resistance to an antibiotic, or a fragment, allelic variant, splice variant or mutein thereof, and at least one sequence comprising SEQ ID NO: 1, 2 or 3, preferably for producing a protein of interest (POI). The inventive chimeric selection marker exhibits: (i) a resistance to an antibiotic; and (ii) a fluorescence activity upon binding of a ligand to the sequence comprising SEQ ID NO: 1, 2 or 3. The invention further refers to nucleic acids encoding the inventive fusion protein and to expression vectors, comprising the inventive fusion protein and additionally the protein of interest (POI). Finally, uses of the inventive chimeric selection marker for screening cells for high expression of a protein of interest (POI) are disclosed.

公开(公告)号：EP1549339A4

公开(公告)日：2008-01-23

申请号：EP03774576

申请日：2003-10-06

申请人： UNIV CHICAGO

发明人： ROIZMAN BERNARD ,  ZHOU GOUYING ,  YE GUO JIE

IPC分类号： C07K14/035 ,  C12N15/869 ,  A61K39/245

CPC分类号： C12N15/86 ,  A61K47/642 ,  A61K47/6901 ,  C07K14/005 ,  C07K2319/20 ,  C12N2710/16622 ,  C12N2710/16643 ,  C12N2810/50 ,  C12N2810/852 ,  C12N2810/859

摘要： The invention relates to engineered Herpes simplex virus (HSV) particles that are targeted to one or more specific receptors. Also, recombinant vectors for producing such HSV particles. By reducing the affinity of HSV for its natural receptor(s) and increasing the affinity for a selected receptor, the HSV particles of the invention may be used for targeting cells that express the selected receptor. The ability to selectively target cells renders the HSV particles particularly useful in selectively killing the selected receptor-bearing cells (such as tumor cells), imaging cells bearing the selected receptor, and providing gene replacement therapy to cells bearing the selected receptor.

公开(公告)号：EP1601331A4

公开(公告)日：2007-09-05

申请号：EP04718559

申请日：2004-03-08

申请人： UNIV UTAH RES FOUND

发明人： SAMLOWSKI WOLFRAM ,  ADAMS NATHAN BRADLEY ,  MCGREGOR JOHN

IPC分类号： A61K38/00 ,  B65D51/28 ,  C07K14/55 ,  C07K14/735 ,  C12N5/10 ,  A61K48/00 ,  C07K14/54 ,  C12N15/12 ,  C12N15/26 ,  C12N15/63 ,  C12N15/64

CPC分类号： C07K14/55 ,  A61K38/00 ,  C07K14/70535 ,  C07K2319/03 ,  C07K2319/20

摘要： Compounds, genetic constructs, and cancer treatment methods 035 provided. Expression vectors were designed to express fusion genes including hIL-2 with a Fcϵ-Ϝ transmembrane anchor derived from a subunit of the FC epsilon receptor. mRNA and the IL­2tm fusion protein was expressed in transfected RD995 tumor cells. Expression of the IL­2tm protein on the tumor A511 surface membrane was confirmed by microscopy. RD995 ce11s transfected with IL-2tm or pCMV2b (empty expression vector) were implanted subcutaneously into C3H /HEN mice. Tumors in groups of mice implanted with 106 or 105 RD995 ce11s transfected with IL-2tm grew slower than controls. Without being limited to any one theory, it is believed that selective expression of cytokines such as IL-2 on the surface of tumors is likely to stimulate tumor-infiltrating lymphocytes that are primed and already recognize tumor antigens, enhancing tumor recognition and killing, potentially avoiding toxicity associated with known cytokine therapies.

公开(公告)号：EP1674575A3

公开(公告)日：2007-08-08

申请号：EP05023967.2

申请日：2001-04-11

申请人： LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY

发明人： Ware, Carl F.

IPC分类号： C12N15/12 ,  C12N15/62 ,  C07K14/705 ,  C07K16/08 ,  A61K38/17 ,  A61K31/713 ,  A61K39/395

CPC分类号： C07K14/705 ,  C07K14/47 ,  C07K16/2878 ,  C07K2317/74 ,  C07K2319/20 ,  C07K2319/30

摘要： A novel polypeptide ligand, p30, or LIGHT, for herpes virus entry mediator, HVEM, is provided. p30 is useful for modulating immune responses and in inhibiting infection and/or subsequent proliferation by herpes virus. HVEM fusion proteins are also provided. Methods for treating subjects with lymphoid cell disorders, tumors, autoimmune diseases, inflammatory disorders of those having or suspected of having a herpes virus infection, utilizing p30 and the fusion proteins of the invention, are also provided.

公开(公告)号：EP1734122A3

公开(公告)日：2007-07-11

申请号：EP06013720.5

申请日：1997-01-08

申请人： GENENTECH, INC.

发明人： Ashkenazi, Avi J. ,  Chuntharapai, Anan ,  Kim, Kyung Jin

IPC分类号： C12N15/28 ,  C07K14/525 ,  C12N15/62 ,  C12N15/86 ,  C12N5/10 ,  A61K38/19 ,  C07K16/24 ,  C12N5/18 ,  A01K67/027

CPC分类号： C07K14/70575 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/20 ,  Y10S930/14 ,  Y10S930/144

摘要： A cytokine, designated Apo-2 ligand, which induces mammalian cell apoptosis is provided. The Apo-2 ligand is believed to be a member of the TNF cytokine family. Compositions including Apo-2 ligand chimeras, nucleic acid encoding Apo-2 ligand, and antibodies to Apo-2 ligand are also provided. Methods of using Apo-2 ligand to induce and to treat pathological conditions such as cancer, are further provided.

公开(公告)号：EP1730278A4

公开(公告)日：2007-07-11

申请号：EP04725553

申请日：2004-04-02

申请人： NEXGEN BIOTECHNOLOGIES INC

发明人： LEE SUN ,  YOO JAE-GEUN ,  CHANG SUXO

IPC分类号： C12N15/11 ,  A01H1/00 ,  C07K4/10 ,  C07K14/46 ,  C12N5/04 ,  C12N15/62 ,  C12N15/63 ,  C12N15/82

CPC分类号： C07K14/461 ,  C07K2319/20 ,  C12N15/62 ,  C12N15/8257

摘要： The present invention relates to a method for the production, isolation, and purification of a recombinant protein, more particularly, to a method for isolating and purifying a foreign protein stably using Anti-Freeze Protein (AFP), thereby producing the protein. The present invention provides a method for the production, isolation and purification of a foreign target protein using its recombinant protein containing AFP, and a construct, an expression vector, a transformant and a recombinant protein. The recombinant protein produced by the present invention shows the biological property and function identical to a naturally occurring protein. Particularly, the present invention is advantageous for the expression and purification of a useful protein.

公开(公告)号：EP1765993A2

公开(公告)日：2007-03-28

申请号：EP05759289.1

申请日：2005-06-03

申请人： Neose Technologies, Inc.

发明人： JOHNSON, Karl F. ,  HAKES, David ,  WEI, Ge ,  LIU, Li ,  SARIBAS, Sami ,  SJOBERG, Eric ,  CLAUSEN, Henrik ,  BENNETT, Eric, Paul ,  MOBASSERI, Aliakbar

IPC分类号： C12N9/10

CPC分类号： C12N9/1081 ,  C07K2319/20 ,  C07K2319/40

摘要： The present invention features compositions and methods related to truncated mutants of ST6GalNAcI. In particular, the invention features truncated human, mouse, and chicken ST6GalNAcI polypeptides. The invention also features nucleic acids encoding such truncated polyeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.

公开(公告)号：EP1734122A2

公开(公告)日：2006-12-20

申请号：EP06013720.5

申请日：1997-01-08

申请人： GENENTECH, INC.

发明人： Ashkenazi, Avi J. ,  Chuntharapai, Anan ,  Kim, Kyung Jin

IPC分类号： C12N15/28 ,  C07K14/525 ,  C12N15/62 ,  C12N15/86 ,  C12N5/10 ,  A61K38/19 ,  C07K16/24 ,  C12N5/18 ,  A01K67/027

CPC分类号： C07K14/70575 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/20 ,  Y10S930/14 ,  Y10S930/144

摘要： A cytokine, designated Apo-2 ligand, which induces mammalian cell apoptosis is provided. The Apo-2 ligand is believed to be a member of the TNF cytokine family. Compositions including Apo-2 ligand chimeras, nucleic acid encoding Apo-2 ligand, and antibodies to Apo-2 ligand are also provided. Methods of using Apo-2 ligand to induce and to treat pathological conditions such as cancer, are further provided.

摘要翻译： 提供了诱导哺乳动物细胞凋亡的称为Apo-2配体的细胞因子。 Apo-2配体被认为是TNF细胞因子家族的成员。 还提供了包含Apo-2配体嵌合体，编码Apo-2配体的核酸和Apo-2配体的抗体的组合物。 进一步提供使用Apo-2配体诱导和治疗病理状况如癌症的方法。

公开(公告)号：EP0832225B1

公开(公告)日：2006-10-11

申请号：EP96919035.4

申请日：1996-06-03

申请人： TRANSKARYOTIC THERAPIES, INC.

发明人： HEARTLEIN, Michael, W. ,  LEMONTT, Jeffrey, F. ,  CONCINO, Michael, F.

IPC分类号： C12N15/12 ,  C12N15/62 ,  C12N15/85 ,  C12N5/10 ,  C07K14/79 ,  A61K38/17 ,  A61K31/70 ,  A61K47/48

CPC分类号： C07K14/79 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/74 ,  C12N15/62

摘要： Chimeric proteins useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.