公开(公告)号：EP2990414A4

公开(公告)日：2016-11-09

申请号：EP14787823

申请日：2014-04-18

申请人： SPIBER INC

发明人： OSAWA TOSHIAKI ,  MORITA KEISUKE

IPC分类号： C07K14/435 ,  A61K8/02 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K9/51 ,  A61K47/42 ,  A61Q19/00 ,  C08J3/05 ,  C08J3/07 ,  C08J3/12 ,  C08L89/00 ,  C09D7/12

CPC分类号： C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K9/5169 ,  A61K47/42 ,  A61K2800/412 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  C08J3/05 ,  C08J3/07 ,  C08J3/12 ,  C08J2389/00 ,  C08L89/00 ,  C09D7/125 ,  C09D7/1275 ,  C09D7/65 ,  C09D7/68

摘要： Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.

摘要翻译： 本发明的多肽粒子是来自蜘蛛丝蛋白质的多肽的粒子，平均粒径为1000nm以下。 本发明的多肽粒子的制造方法包括：溶液制备步骤，其中将多肽溶解于选自DMSO，DMF中的至少一种溶剂中，并将其与无机盐溶解，得到溶液 的多肽; 在溶液制备工序中产生的溶液用水溶性溶剂代替以获得多肽的水溶液的步骤; 以及将多肽的水溶液干燥的步骤。 因此，本发明提供适用于生物体并能够应用于化妆品等的多肽颗粒，同时鉴定多肽颗粒的性质及其制备方法。

公开(公告)号：EP2940066A4

公开(公告)日：2016-07-06

申请号：EP13867491

申请日：2013-12-17

申请人： SPIBER INC

发明人： SEKIYAMA KAORI ,  ISHIKAWA MIZUKI ,  MURATA SHINYA

IPC分类号： C08J5/18 ,  B29C39/02 ,  B29C55/02 ,  B29C71/02 ,  B29L7/00 ,  C07K14/435 ,  C08L89/00

CPC分类号： C07K14/43518 ,  B29C39/02 ,  B29C55/02 ,  B29C71/02 ,  B29K2089/00 ,  B29K2867/003 ,  B29K2995/002 ,  B29L2007/008 ,  C08J5/18 ,  C08J2389/00 ,  C08L89/00

摘要： A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260°C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

公开(公告)号：EP2397543B1

公开(公告)日：2015-05-27

申请号：EP09839182.4

申请日：2009-01-29

申请人： Spiber Inc.

发明人： SUGAHARA, Junichi ,  SEKIYAMA, Kazuhide

IPC分类号： C12N15/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12Q1/68

CPC分类号： C12N15/1065 ,  G06F19/22

公开(公告)号：EP2774934A4

公开(公告)日：2015-05-13

申请号：EP12844908

申请日：2012-10-29

申请人： SPIBER INC

发明人： SUGAHARA JUNICHI ,  SEKIYAMA KAORI ,  SATO RYOTA ,  ISHIKAWA MIZUKI ,  MURATA SHINYA ,  SEKIYAMA KAZUHIDE ,  OTOMO KAZUKO

IPC分类号： C07K14/435 ,  C12N15/09 ,  D01D5/04 ,  D01F4/02

CPC分类号： C08K5/41 ,  B29D99/0078 ,  C07K14/43518 ,  C08K3/16 ,  C08K5/20 ,  D01D5/04 ,  D01F4/02 ,  D01F6/68 ,  Y10S530/858

摘要： A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(ii): (i) DMSO with an inorganic salt; and (ii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

摘要翻译： 本发明的多肽溶液是多肽溶液，其中衍生自天然蜘蛛丝蛋白的多肽溶解在溶剂中。 溶剂含有选自以下（i） - （iii）中的至少一种：（i）DMSO; （ii）具有无机盐的DMSO; 和（iii）具有无机盐的DMF。 此外，在本发明中，通过以下方法获得人造多肽纤维：使用多肽溶液作为掺杂溶液; 并将喷丝头溶液从喷丝头挤出到去溶剂浴中，以从溶剂溶液中除去溶剂，并形成纤维以产生未拉伸的纱线。 此外，在本发明中，通过使多肽溶液进行热处理，然后从其中除去未溶解的物质来纯化多肽。 因此，本发明提供了其溶质具有高溶解度的多肽溶液，本身的溶剂本身是低成本的，并且允许在高温下溶解并且具有高安全性：制备人造多肽纤维的方法和纯化多肽的方法。

公开(公告)号：EP2716798A1

公开(公告)日：2014-04-09

申请号：EP12793074.1

申请日：2012-05-30

申请人： Spiber Inc.

发明人： SEKIYAMA Kazuhide ,  SEKIYAMA Kaori ,  ISHIKAWA Mizuki ,  SATO Ryota ,  MURATA Shinya

IPC分类号： D01F4/00 ,  D01D5/06 ,  C12P21/02

摘要： An artificial polypeptide fiber of the present invention is an artificial fiber containing a polypeptide as a main component, and has a stress of 350 MPa or more and a toughness of 138 MJ/m 3 or more. A method for producing an artificial polypeptide fiber of the present invention is a method for producing the artificial polypeptide fiber obtained by spinning a spinning solution (6) containing a polypeptide derived from natural spider silk proteins and performing drawing of at least two stages. The drawing of at least two stages includes a first-stage drawing (3) in wet heat and a second-stage drawing (4) in dry heat. Thereby, the present invention provides high-toughness artificial polypeptide fibers having favorable stress and rupture elongation, and a method for producing the same.

摘要翻译： 本发明的人造多肽纤维是以多肽为主成分的人造纤维，其应力为350MPa以上，韧性为138MJ / m 3以上。 本发明的人造多肽纤维的制造方法是通过纺丝含有天然蜘蛛丝蛋白质的多肽的纺丝溶液（6）而得到的人造多纤维的制造方法，并进行至少两个阶段的拉伸。 至少两个阶段的绘图包括在湿热中的第一阶段绘图（3）和在干热中的第二阶段绘图（4）。 因此，本发明提供了具有良好的应力和断裂伸长率的高韧性人造多孔纤维及其制造方法。