公开(公告)号：EP0230430A4

公开(公告)日：1989-03-09

申请号：EP86903165

申请日：1986-06-12

申请人： LUMINIS PTY LTD ,  QUINN PATRICK JAMES

发明人： QULNN PATRICK JAMES

IPC分类号： C12N5/075

CPC分类号： C12N5/0609 ,  C12N2500/05 ,  C12N2500/34

摘要： A culture medium for in vitro fertilization of human oocytes which comprises: NaCl (96.5 - 106.7 mM) KCl(4.46 - 4.92 mM) MgSO4.7H2O (0.18 - 0.22 mM) KH2PO4 (0.35 - 0.39 mM) CaCl2.2H2O (1.94 - 2.14 mM) NaHCO3 (23.7 - 26.3 mM) Glucose (2.64 - 2.92 mM) Sodium Pyruvate (0.31 - 0.35 mM) Sodium Lactate (20.3 - 22.5 mM) Penicillin (95 - 105 units/ml) Phenol red (5 - 15 micrograms/ml). Culture media wherein the ratio of sodium ions to potassium ions is in the range of from 28 to 32 are preferred.

公开(公告)号：EP2801363B1

公开(公告)日：2018-02-21

申请号：EP14002489.4

申请日：2005-03-29

申请人： Inguran, LLC

发明人： Crowley, Kathleen S. ,  Ludwig, Cindy L. ,  Graham, Jeffrey A.

IPC分类号： A61K35/52 ,  C12N5/076 ,  G01N1/30

CPC分类号： A61K35/52 ,  A01N1/021 ,  A01N1/0226 ,  C12N5/061 ,  C12N5/0611 ,  C12N2500/05 ,  G01N1/30

摘要： Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.

公开(公告)号：EP2593542B1

公开(公告)日：2018-01-03

申请号：EP11738896.7

申请日：2011-07-13

申请人： Anthrogenesis Corporation

发明人： HARIRI, Robert, J. ,  HEIDARAN, Mohammad, A. ,  JASKO, Stephen ,  KANG, Lin ,  LAW, Eric ,  PAL, Ajai ,  STOUT, Bhavani ,  VOSKINARIAN-BERSE, Vanessa ,  ZEITLIN, Andrew ,  ZHANG, Xiaokui

IPC分类号： C12N5/0783 ,  A61K35/12 ,  A61K35/17

CPC分类号： C12N5/0646 ,  A61K35/17 ,  A61K2035/124 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/2302 ,  C12N2501/2303 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/26 ,  C12N2501/59 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2506/11

摘要： Provided herein are in vitro two-step methods of producing populations of activated natural killer (NK) cells, comprising an expansion and a differentiation step. Also provided herein are populations of activated NK cells obtained by the method of the invention, and the use of the same in methods for treating cancer in an individual. Provided herein are also in vitro methods of suppressing the proliferation of tumor cells comprising contacting the tumor cells with a population of NK produced by the methods provided herein.

公开(公告)号：EP3237109A1

公开(公告)日：2017-11-01

申请号：EP15828501.5

申请日：2015-12-21

申请人： UPM-Kymmene Corporation

发明人： NUOPPONEN, Markus ,  KAJANTO, Isko ,  MERILUOTO, Anne ,  LUUKKO, Kari ,  PAASONEN, Lauri

IPC分类号： B01J13/00

CPC分类号： B01J13/0069 ,  B01J13/0056 ,  C12N1/22 ,  C12N5/0018 ,  C12N2500/05 ,  C12N2500/34 ,  C12N2521/00 ,  C12N2533/78 ,  D21H11/18 ,  D21H11/20

摘要： The invention relates to a method for reducing the viscosity of a nanofibrillar cellulose hydrogel, wherein the method comprises mixing a nanofibrillar cellulose hydrogel with an aqueous growth medium for cell culture, wherein the aqueous growth medium contains one or more salts and optionally one or more sugars, using shearing forces so that a homogeneous dispersion is formed. The invention further relates to a dispersion comprising a nanofibrillar cellulose hydrogel and an aqueous growth medium for cell culture and to a use of an aqueous growth medium.

摘要翻译： 本发明涉及一种降低纳米纤维状纤维素水凝胶粘度的方法，其中所述方法包括将纳米纤维状纤维素水凝胶与用于细胞培养的含水生长培养基混合，其中所述含水生长培养基含有一种或多种盐和任选的一种或多种糖 ，使用剪切力以形成均匀的分散体。 本发明进一步涉及包含纳米纤维状纤维素水凝胶和用于细胞培养的含水生长培养基的分散体以及含水生长培养基的用途。

公开(公告)号：EP2986715A4

公开(公告)日：2016-11-02

申请号：EP14785372

申请日：2014-04-17

申请人： SALK INST FOR BIOLOGICAL STUDI

发明人： GAGE FRED H ,  BARDY CEDRIC

IPC分类号： C12N5/079 ,  C12N5/0793 ,  C12N5/0797

CPC分类号： C12N5/0619 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/13 ,  C12N2501/39

摘要： Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.

公开(公告)号：EP3083978A1

公开(公告)日：2016-10-26

申请号：EP14870754.0

申请日：2014-12-19

申请人： Essential Pharmaceuticals, LLC

发明人： ELHOFY, Adam ,  WEBER, Allan

IPC分类号： C12P21/04 ,  C12N5/00 ,  A61K9/127

CPC分类号： C12N5/0018 ,  A01N1/0221 ,  A01N1/0226 ,  C12N5/0037 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/50 ,  C12N2500/62 ,  C12N2501/39 ,  C12N2533/32

摘要： The present disclosure relates, in general, to a media, e.g., a serum replacement, media supplement, complete media or cryopreservation media, comprising a base physiological buffer and liposomes comprising cholesterol, phosphatidylcholine and fatty acids. It is contemplated that media provides advantages to improve cell growth in culture compared to cells cultured not using the serum replacement described herein.

公开(公告)号：EP2882846A4

公开(公告)日：2016-07-06

申请号：EP13799830

申请日：2013-06-05

申请人： UNIV CALIFORNIA

发明人： CLEGG DENNIS ,  BUCHHOLZ DAVID ,  CROZE ROXANNE ,  PENNINGTON BRITNEY ,  COFFEY PETER ,  LEACH LYNDSAY

IPC分类号： C12N5/02 ,  C12N5/079 ,  C12N5/0793

CPC分类号： C12N5/0621 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/35 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/02

摘要： The invention relates to the field of cell culture, specifically, the derivation of retinal pigmented epithelial cells from pluripotent cells. The invention comprises the use of various cell culture medium supplements, medium formulations, and methods of using such medium supplements and formulations, in order to effect the rapid differentiation of pluripotent cells into retinal pigmented epithelial cells with very high yields. The invention further includes cell culture media formulations for the efficient maintenance, propagation, and maturation of cultured retinal pigmented epithelial cells.

摘要翻译： 本发明涉及细胞培养领域，具体地，从多能细胞视网膜色素上皮细胞的推导。 本发明包括使用各种不同的细胞培养基补充物，培养基配方蒸发散，并使用搜索介质补充剂和式蒸发散的方法中，为了以非常高的产率，以实现多能干细胞的快速分化成视网膜色素上皮细胞。 本发明还包括用于快捷的维修，增殖和培养的视网膜色素上皮细胞的成熟的细胞培养基的制剂蒸发散。

公开(公告)号：EP2861717B1

公开(公告)日：2016-04-27

申请号：EP13723676.6

申请日：2013-05-17

申请人： Merck Patent GmbH

发明人： VON HAGEN, Joerg

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0018 ,  A61K9/141 ,  A61K9/19 ,  B02C23/00 ,  C12N2500/05 ,  C12N2500/10

公开(公告)号：EP2986715A1

公开(公告)日：2016-02-24

申请号：EP14785372.5

申请日：2014-04-17

申请人： Salk Institute for Biological Studies

发明人： GAGE, Fred, H. ,  BARDY, Cedric

IPC分类号： C12N5/079 ,  C12N5/0793 ,  C12N5/0797

CPC分类号： C12N5/0619 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/13 ,  C12N2501/39

摘要： Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.

摘要翻译： 此处提供的是尤其是用于培养神经细胞的培养基组合物。 具体而言，本文提供的组合物模拟活脑中的重要生理条件并维持神经活性。 本文提供的培养基组合物在长期体外培养中提高人神经元成熟的效率并促进突触功能。

公开(公告)号：EP2865749A4

公开(公告)日：2015-12-30

申请号：EP13810037

申请日：2013-05-23

申请人： RA JEONG CHAN ,  STEMCELL CO LTD K

发明人： KANG SUNG KEUN ,  JO JUNG YOUN ,  RA JEONG CHAN

IPC分类号： C12N5/0775 ,  C12N5/02

CPC分类号： C12N5/0667 ,  C12N2500/05 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/999 ,  C12N2511/00