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205 条记录 (耗时 0.016 秒)

    NOVEL METHOD OF INDUCING THE DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO ECTODERMAL CELLS AND USE THEREOF
    72.
    发明公开
    NOVEL METHOD OF INDUCING THE DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO ECTODERMAL CELLS AND USE THEREOF 审中-公开
    FOR分化诱导胚胎干细胞和新方法外胚层细胞及其用途

    公开(公告)号：EP1302533A1

    公开(公告)日：2003-04-16

    申请号：EP01930185.2

    申请日：2001-05-16

    申请人： KYOWA HAKKO KOGYO CO., LTD. ,  Sasai, Yoshiki

    发明人： SASAI, Yoshiki Kyoto University ,  NISHIKAWA, Shin-Ichi Kyoto University

    IPC分类号： C12N5/06 ,  C07K16/18 ,  C12P21/08 ,  C12Q1/02 ,  G01N33/50 ,  G01N33/15 ,  A61K35/48 ,  A61K35/30 ,  A61K35/36 ,  A61P25/28 ,  A61P25/00 ,  A61P17/00

    CPC分类号： C12N5/0619 ,  A61K2039/505 ,  C07K16/18 ,  C12N2501/155 ,  C12N2502/13 ,  C12N2506/02

    摘要： A method for inducing differentiation of an embryonic stem cell into an ectodermal cell and an ectoderm-derived cell, which comprises culturing the embryonic stem cell under non-aggregation conditions; a medium and a medium supernatant used in the method; an agent for inducing differentiation used in the method; a stroma cell or a stroma cell-derived factor having activity of inducing differentiation in the method; an antibody which specifically recognizes the stroma cell; an antigen which recognizes the antibody; a cell induced by the method; a method for evaluating or screening a substance relating to the regulation in a differentiation step from an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell by carrying out the method; and a medicament comprising the stroma cell, the stroma cell-derived cell, the antibody, the antigen or the cell.

    摘要翻译： 一种诱导胚胎干细胞分化为外胚层在细胞和外胚层到衍生的细胞，包括培养的非聚合条件下，胚胎干细胞的方法; 一介质和该方法中使用的培养基上清液; 用于诱导的方法中使用分化的试剂; 一个基质细胞或具有诱导在所述方法分化的活性的基质细胞衍生因子; 抗体特异性地识别所述基质细胞; 抗原识别所述抗体的; 由该方法诱导的细胞; 用于筛选的物质或评估有关的调节在分化步骤中从胚胎干细胞成外胚层细胞或在通过执行该方法外胚层来源的细胞的方法; 和药物，其包含所述基质细胞，间质细胞来源的细胞，所述抗体，抗原或细胞。

    Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof
    73.
    发明公开
    Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof 失效
    组织衍生的肿瘤生长抑制剂，其制备方法及其用途

    公开(公告)号：EP0200090A3

    公开(公告)日：1988-05-04

    申请号：EP86105211

    申请日：1986-04-15

    申请人： Oncogene Science, Inc.

    发明人： Iwata, Kenneth K. ,  Stephenson, John R. ,  Gold, Leslie I.

    IPC分类号： A61K37/02 ,  A61K35/48 ,  A61K35/50 ,  A61K35/12 ,  C07K03/02 ,  G01N33/68

    CPC分类号： C07K14/495 ,  A61K38/00

    摘要： An acidified, ethanol extract derived from human tissue which comprises a plurality of acidic polypeptides, each of which has a molecular weight less than about 20,000 daltons and each of which has the property of inhibiting the growth of human tumor cells and of an established mink lung cell line - (CCL64) while stimulating the growth of normal human foreskin fibroblasts is disclosed. The inhibitory activity against human tumor cell growth is not destroyed upon increasing the temperature of the acidified, ethanol extract to about 100°C for about 3 minutes or adding acetic acid until the acidified, ethanol extract is up to about 1.0 molar in acetic acid. Further, the activity of the acidified, ethanol extract is enhanced when the acidified, ethanol extract is prepared at about 4°C rather than about at 23°C. An acidified, ethanol extract derived from human umbilical cord which has been treated to removed substantially all blood, all extracellular soluble components and substantially all intracellular soluble components which comprises at least one acidic polypeptide having an apparent molecular weight less than about 30,000 daltons, compositions of matter designated tissue-derived growth inhibitor-1 (TGI-1), tissue-derived growth inhibitor (TGI), and tissue-derived growth inhibitor-2 (TGI-2) and a heterogeneous population of polypeptides designated CM-I, CM-II, CM-III and CM-IV, are also disclosed as are methods of preparation, pharmaceutical compositions which include, and uses of the foregoing.

    PROCESSING AND USE OF FLUIDS OF THE REPRODUCTIVE TRACT FOR IMPROVING THE IN VITRO PRODUCTION OF EMBRYOS OF MAMMALS
    79.
    发明公开
    PROCESSING AND USE OF FLUIDS OF THE REPRODUCTIVE TRACT FOR IMPROVING THE IN VITRO PRODUCTION OF EMBRYOS OF MAMMALS 审中-公开
    繁殖期流体的加工和使用以改善哺乳动物胚胎的体外生产

    公开(公告)号：EP3205716A1

    公开(公告)日：2017-08-16

    申请号：EP15848765.2

    申请日：2015-10-07

    申请人： Universidad de Murcia

    发明人： ROMAR ANDRES, Raquel ,  COY FUSTER, María Pilar

    IPC分类号： C12N5/073 ,  A61K35/48 ,  A61P15/08

    CPC分类号： C12M21/06 ,  A01K67/00 ,  A01K67/02 ,  A61B17/43 ,  A61K35/48 ,  B01L3/50 ,  B01L2200/06 ,  C12M1/00 ,  C12N5/0604 ,  C12N2517/10 ,  C12Q1/02

    摘要： Processing and use of fluids from the reproductive tract (biofluids) to improve the in vitro production of mammalian embryos comprising the following steps: a) fractionation and processing of biofluids through a sorting, purification, lyophilization and subsequent storage; b) a method of sperm capacitation in a culture medium supplemented with biofluids; c) in vitro fertilization in a medium enriched with biofluids and d) subsequent in vitro culture with development of the obtained embryos to any stage of preimplantational development in culture media supplemented with biofluids.

    摘要翻译： 处理和使用来自生殖道的流体（生物流体）以改善哺乳动物胚胎的体外生产，包括以下步骤：a）通过分选，纯化，冻干和随后的储存分馏和处理生物流体; b）补充生物流体的培养基中的精子获能方法; c）在富含生物流体的培养基中进行体外受精，和d）随后的体外培养以及获得的胚胎发育至补充有生物流体的培养基中的任何植入前发育阶段。