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    Recombinant PRRS proteins, diagnostic kits and vaccines containing said recombinant proteins
    71.
    发明公开
    Recombinant PRRS proteins, diagnostic kits and vaccines containing said recombinant proteins 失效
    重组PRRS蛋白，诊断试剂盒和含有所述重组蛋白的疫苗

    公开(公告)号：EP1469076A3

    公开(公告)日：2004-12-08

    申请号：EP04076580.2

    申请日：1995-05-10

    申请人： Wyeth Farma, S.A.

    发明人： Plana Duran, Juan ,  Casal Alvarez, José Ignacio ,  Climent Sanchez, Isabel

    IPC分类号： C12N15/40 ,  C07K14/08 ,  C12N7/01 ,  A61K39/12 ,  G01N33/569

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2770/10022

    摘要： The present invention discloses the production of recombinant proteins of the virus causing the porcine respiratory and reproductive syndrome (PRRS), corresponding to the OFR s 2-7 of the PRRSV isolated in Spain, (PRRS-Olot), in a system of expression of recombinant baculoviruses multiplided in a cell culture of a permissive host . Said recombinant proteins are appropriate to formulate vaccines capable of efficiently protecting pigs'against PRRS as well as to prepare diagnostic kits appropriate to detect both the presence of antibodies which recognize PRRSV and the presence of PRRSV in a porcine biological sample. This invention applies to veterinary.

    摘要翻译： 本发明公开了使所述猪呼吸和生殖综合征（PRRS）的生产所述病毒的重组蛋白，对应于OFRS在西班牙，（PRRS-奥洛特）中分离的PRRSV的2-7，在重组的表达的系统 杆状病毒在允许宿主的细胞培养物中多倍化。 所述重组蛋白适于配制能够有效保护猪对PRRS的免疫性疫苗，以及制备适于检测识别PRRSV的抗体的存在和PRRSV在猪生物样品中的存在的诊断试剂盒。 本发明适用于兽医。

    Arterivirus marker vaccine
    72.
    发明公开
    Arterivirus marker vaccine 审中-公开
    动脉炎病毒Markierungsimpfstoff

    公开(公告)号：EP1438969A1

    公开(公告)日：2004-07-21

    申请号：EP03075117.6

    申请日：2003-01-14

    申请人： Universiteit Utrecht

    发明人： The designation of the inventor has not yet been filed

    IPC分类号： A61K39/12 ,  C07K14/08 ,  G01N33/569 ,  C07K16/10

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10021 ,  C12N2770/10022 ,  C12N2770/10034 ,  G01N33/56983

    摘要： The invention relates to arterivirus compositions used as vaccines to induce protective immunity against infection or disease caused by arteriviruses. Provided is a vaccine directed against infection or disease caused by an arterivirus, said vaccine comprising an arterivirus provided with a modification in a nucleic acid sequence allowing to distinguish animals vaccinated with said vaccine from animals infected with wild-type arterivirus or vaccinated with an unmodified arterivirus strain. Also provided is a method for distinguishing unvaccinated animals or animals vaccinated with a vaccine according to the invention from animals infected with wild-type arterivirus or vaccinated with an unmodified arterivirus strain.

    摘要翻译： 本发明涉及用作诱导针对动脉病毒引起的感染或疾病的保护性免疫的疫苗的动脉病毒组合物。 提供针对由动脉病毒引起的感染或疾病的疫苗，所述疫苗包含在核酸序列中提供修饰的动脉病毒，其允许将用所述疫苗接种的动物与用野生型动脉病毒感染的动物区分或用未修饰的动脉病毒 应变。 还提供了用根据本发明的疫苗接种的未接种疫苗的动物或动物从用野生型动脉病毒感染的动物或用未修饰的动脉病毒株接种的方法。

    Adaptation sites of PRRSV
    74.
    发明公开
    Adaptation sites of PRRSV 有权
    Sequenz定位zur Anpassung bei PRRSV

    公开(公告)号：EP1350840A1

    公开(公告)日：2003-10-08

    申请号：EP02076334.8

    申请日：2002-04-05

    申请人： ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V.

    发明人： van Rijn, Petrus Antonius ,  Meulenberg, Johanna Jacoba Maria

    IPC分类号： C12N7/01 ,  C12N7/04 ,  C07K14/08

    CPC分类号： C07K14/005 ,  A61K2039/522 ,  C12N7/00 ,  C12N2770/10021 ,  C12N2770/10022 ,  C12N2770/10061 ,  C12N2770/10064

    摘要： The invention relates to the field of virology , more in particular to the field of vaccine production, more specifically to the in vitro propagation of virus, more specifically to the adaptation of virus to a cell line.
    The invention provides a method to determine the capability of an arterivirus to replicate in a green monkey cell line, comprising determining the amino acids at positions, which correspond to the amino acid positions 75-107 of GP2a of PRRSV isolate I-1102, more specifically amino acid position 88 and/or amino acid position 95 of said GP2a. The invention further discloses a method to produce arterivirus in green monkey cell line wherein the virulence of said arterivirus is maintained, with increased virus yield.
    The invention further provides a method to determine the attenuation of an arterivirus, comprising determining the amino acids at positions, which correspond to the amino acid positions 121-148 of GP5 of PRRSV isolate I-1102, more specifically amino acid 136 of said GP5 and/or determining the amino acids at positions, which correspond to the amino acid positions 651-675 and/or amino acid positions 2331-2355 of ORF1ab of PRRSV isolate I-1102, more specifically amino acid 663 and/or 2343 of said ORF1ab. The invention also provides a method to attenuate the virulence of said arterivirus by changing said amino acids.

    摘要翻译： 本发明涉及病毒学领域，更具体地涉及疫苗生产领域，更具体地涉及病毒的体外繁殖，更具体地涉及将病毒适应于细胞系。 本发明提供一种确定动物病毒在绿猴细胞系中复制的能力的方法，其包括确定与PRRSV分离株I的GP2a的氨基酸位置75-107相对应的位置处的氨基酸， 1102，更具体地说是GP2a的氨基酸位置88和/或氨基酸位置95。 本发明还公开了一种在绿猴细胞系中产生动脉病毒的方法，其中维持所述动脉病毒的毒力，病毒产量增加。 本发明进一步提供了确定动脉病毒衰减的方法，其包括确定对应于PRRSV分离物I-1102的GP5的氨基酸位置121-148的位置的氨基酸，更具体地说，氨基酸136 的所述GP5和/或确定对应于PRRSV分离物I-1102的ORF1ab的氨基酸位置651-675和/或氨基酸位置2331-2355的位置的氨基酸，更具体地，氨基酸663和/或2343 的ORF1ab。 本发明还提供了通过改变所述氨基酸来减弱所述动脉病毒的毒力的方法。

    METHOD OF GENETIC MODIFICATION OF A WILD TYPE VIRAL SEQUENCE
    79.
    发明公开
    METHOD OF GENETIC MODIFICATION OF A WILD TYPE VIRAL SEQUENCE 有权
    一种野生型病毒序列的遗传修正方法

    公开(公告)号：EP1144661A2

    公开(公告)日：2001-10-17

    申请号：EP99930957.8

    申请日：1999-07-09

    申请人： Ses Europe N.V./S.A.

    发明人： LAUBER, E.Résidence les Ormes 2, BAT. D2, APPT 11 ,  GUILLEY, Hubert ,  RICHARDS, Ken ,  JONARD, Gérard

    IPC分类号： C12N15/82 ,  C07K14/08 ,  C12N5/10 ,  C12Q1/68 ,  A01H5/00

    CPC分类号： C07K14/005 ,  C12N15/8283 ,  C12N2770/00022

    摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon; and recovering said specifically genetically modified TGB-3 viral sequence.

    摘要翻译： 本发明涉及TGB-3野生型病毒序列的遗传修饰的方法，用于减少或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农艺学性质可能的有害影响，所述方法包括以下连续步骤 ：将所述序列提交至允许将至少一个氨基酸置换为不同氨基酸的点突变; 选择具有所述点突变且不能促进具有功能失调的TGB-3野生型病毒序列的突变病毒的细胞间移动的遗传修饰的TGB-3野生型病毒序列， 复制品; 在所述遗传修饰的TGB-3病毒序列中进一步选择当从复制子表达突变体形式时抑制共同接种的野生型病毒感染的特异性遗传修饰的序列; 并回收所述特异性遗传修饰的TGB-3病毒序列。