公开(公告)号：EP1404827A2

公开(公告)日：2004-04-07

申请号：EP02701241.8

申请日：2002-02-25

申请人： Novozymes A/S

发明人： TSUTSUMI, Noriko ,  VIND, Jesper ,  PATKAR, Shamkant, Anant

IPC分类号： C12N9/20 ,  C12N15/10

CPC分类号： C11D3/386 ,  A21D8/042 ,  C11D3/38627 ,  C12N9/20 ,  C12N15/1027

摘要： The inventors have isolated novel genes with a high homology to the T. lanuginosus lipase gene and are thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The expressed proteins may further be screened to identify lipolytic enzymes with improved properties. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

公开(公告)号：EP1309674A1

公开(公告)日：2003-05-14

申请号：EP01960183.0

申请日：2001-07-19

申请人： Novozymes A/S

发明人： STERGAARD, Peter, Rahbek ,  HJORT, Carsten, M. ,  DEUSSEN, Heinz-Josef ,  ZUDEL, Magali ,  EBDRUP, S ren ,  CHRISTENSEN, S ren ,  PATKAR, Shamkant, Anant

IPC分类号： C12N9/16 ,  C07K14/38 ,  C12P41/00 ,  C07B57/00 ,  C12P7/42 ,  C07C51/09

CPC分类号： C12N9/16 ,  C12N9/18 ,  C12P7/40 ,  C12P41/005

摘要： An esterase isolated from Aspergillus oryzae is capable of stereoselective hydrolysis of chiral esters and also has arylesterase activity (EC 3.1.1.2) and feruloyl esterase activity (EC 3.1.1.73). The esterase has only a limited homology to known amino acid sequences.

公开(公告)号：EP0984703B1

公开(公告)日：2003-04-09

申请号：EP98922343.3

申请日：1998-05-15

申请人： Novozymes Biotech, Inc. ,  Novozymes A/S ,  ASAHI CHEMICAL INDUSTRY CO., LTD.

发明人： BLINKOVSKY, Alexander ,  BROWN, Kimberly ,  GOLIGHTLY, Elizabeth ,  BYUN, Tony ,  MATHIANSEN, Thomas, E. ,  KOFOD, Lene, V. ,  FUJII, Mikio ,  MARUMOTA, Chigusa

IPC分类号： A23J3/34 ,  C12N9/48 ,  A23L1/305 ,  A23K1/16

CPC分类号： A21D2/267 ,  A21D2/268 ,  A23J3/34 ,  A23L27/21 ,  C12N9/48 ,  C12P21/06

公开(公告)号：EP1280919A2

公开(公告)日：2003-02-05

申请号：EP01927640.1

申请日：2001-04-30

申请人： Novozymes A/S

发明人： ROGGEN, Erwin Ludo

IPC分类号： C12N15/55 ,  C12N9/20 ,  C11D3/386 ,  A21D8/04

CPC分类号： C12N9/54 ,  A21D2/267 ,  A21D8/042 ,  A61K39/00 ,  C07K1/047 ,  C07K5/1008 ,  C07K5/101 ,  C07K5/1013 ,  C07K5/1019 ,  C07K5/1021 ,  C07K14/001 ,  C07K16/40 ,  C07K16/4283 ,  C11D3/38627 ,  C11D3/38645 ,  C12N9/0061 ,  C12N9/20 ,  C12Y110/03002 ,  C12Y304/21062 ,  Y10S435/911

摘要： The properties of a fungal lipolytic enzyme can be altered by substituting amino acid residues corresponding to certain specified amino acid residues in the T. lanuginosus lipase. The altered property may be, e.g., an increased thermostability, an altered pH dependence, or an altered substrate specificity.

公开(公告)号：EP1144658A2

公开(公告)日：2001-10-17

申请号：EP00904346.4

申请日：2000-01-13

申请人： Novozymes Biotech, Inc.

发明人： BERKA, Randy, M. ,  REY, Michael, W. ,  YODER, Wendy, T.

IPC分类号： C12N15/80 ,  C12N15/60 ,  C12N9/88 ,  C12N1/14

CPC分类号： C12R1/77 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02

摘要： The present invention relates to methods for producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a nucleic acid sequence encoding the heterologous polypeptide and (ii) the mutant produces less of the cyclohexadepsipeptide than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the heterologous polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated cyclohexadepsipeptide synthetases and isolated nucleic acid sequences encoding the cyclohexadepsipeptide synthetases. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the cyclohexadepsipeptide synthetases. The present invention further relates to cyclohexadepsipeptides produced by the cyclohexadepsipeptide synthetases.

公开(公告)号：EP1137788A1

公开(公告)日：2001-10-04

申请号：EP99955838.0

申请日：1999-11-12

申请人： Novozymes A/S

发明人： NIELSEN, Jack, Bech ,  KJ RULFF, S ren

IPC分类号： C12N15/82 ,  C12N15/55 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N9/2425 ,  C12N9/2414 ,  C12N9/2417 ,  C12N15/8245

摘要： A transgenic plant cell expressing a maltogenic amylase (such as Novamyl®) or a beta-amylase; a transgenic plant regenerated from said cell; seeds generated from such plant where said seeds comprise a maltogenic amylase or a beta-amylase and the use of said seeds, optionally in ground form, for catalyzing an industrial process, such as e.g. in baking. The maltogenic amylase providing an anti staling effect in bread produced from the seeds in question.

公开(公告)号：EP1113721A1

公开(公告)日：2001-07-11

申请号：EP99945667.6

申请日：1999-09-14

申请人： Novozymes Biotech, Inc.

发明人： XU, Feng

IPC分类号： A21D8/04 ,  A23L1/105 ,  A23L1/03

CPC分类号： C12Y402/02002 ,  A21D8/042

摘要： The present invention relates to methods for preparing a dough, comprising incorporating into the dough an effective amount of one or more pectate lyases which improve one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions comprising an effective amount of one or more pectate lyases for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.

公开(公告)号：EP1056873A2

公开(公告)日：2000-12-06

申请号：EP99911012.5

申请日：1999-02-26

申请人： NOVO NORDISK BIOTECH, INC.

发明人： WIDNER, William ,  SLOMA, Alan ,  THOMAS, Michael, D.

IPC分类号： C12N15/75 ,  C12N15/52 ,  C12N15/57 ,  C12N9/54 ,  C12N1/21

CPC分类号： C12N15/75

摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a 'consensus' promoter having the sequence TTGACA for the '-35' region and TATAAT for the '-10' region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the 'consensus' promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

公开(公告)号：EP1042457A1

公开(公告)日：2000-10-11

申请号：EP98956817.5

申请日：1998-11-13

申请人： NOVO NORDISK A/S ,  NOVO NORDISK BIOTECH, INC. ,  Japan Tobacco Inc.

发明人： BLINKOVSKY, Alexander ,  BYUN, Tony, S. ,  KLOTZ, Alan, V. ,  SLOMA, Alan ,  BROWN, Kimberly ,  TANG, Maria ,  FUJII, Miko ,  MARUMOTO, Chigusa ,  KOFOD, Lene, Venke

IPC分类号： C12N9/48

CPC分类号： A23J3/34 ,  C12N9/48 ,  C12P21/06

摘要： The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

公开(公告)号：EP0730688B1

公开(公告)日：2000-03-08

申请号：EP95901358.2

申请日：1994-11-23

申请人： NOVO NORDISK A/S

发明人： SHARYO, Masaki ,  SHIMOTO, Hidesato ,  SAKAGUCHI, Hiromichi

IPC分类号： D21C5/02 ,  C12S3/08

CPC分类号： D21C5/005 ,  D21C5/025 ,  Y02W30/648

摘要： In the production of pulp and paper from starch-coated paper, the deinking effect can be improved by including a treatment with a starch-degrading enzyme e.g. amylase or a debranching enzyme. The process comprises enzyme treatment before, during or after disintegration of the paper to produce pulp, followed by separation of ink particles.