Abstract:
The present invention relates to a process for preparing a compound having the formula (I): said process comprising the following steps: a) the addition of an oxygen source into a solution of a compound of formula (II) in a water-miscible solvent, b) the addition of a laccase in the solution obtained after step a); and c) the possible recovering of the compound of formula (I) thus obtained.
Abstract:
The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the Bacillus spore coat protein CotA.
Abstract:
The invention relates to methods of preconditioning pretreated lignocellulose-containing material in the presence of a combination of laccase and beta-glucosidase. The invention also relates to processes of producing sugars and fermentation products including a step of preconditioning pretreated lignocellulose-containing material in the presence of a combination of laccase and beta-glucosidase. The invention also relates to compositions suitable for preconditioning.
Abstract:
Provided are isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract:
A recombinant filamentous fungal cell (eg. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to pepAb, derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.
Abstract:
The properties of a fungal lipolytic enzyme can be altered by substituting amino acid residues corresponding to certain specified amino acid residues in the T. lanuginosus lipase. The altered property may be, e.g., an increased thermostability, an altered pH dependence, or an altered substrate specificity.