METHOD FOR IMPROVING THE FERMENTABLE SUGAR YIELD BY USING A BACILLUS SPORE COAT PROTEIN (COTA)
    3.
    发明授权
    METHOD FOR IMPROVING THE FERMENTABLE SUGAR YIELD BY USING A BACILLUS SPORE COAT PROTEIN (COTA) 有权
    使用芽孢杆菌衣壳蛋白(COTA)改进可发酵的糖产量的方法

    公开(公告)号:EP2976432B1

    公开(公告)日:2017-06-14

    申请号:EP13710440.2

    申请日:2013-03-20

    Applicant: MetGen Oy

    Abstract: The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the Bacillus spore coat protein CotA.

    Abstract translation: 本发明涉及用于将生物质转化为碳水化合物的方法,特别是可发酵的糖。 它提供了用于提高生物质的酶消化产率的手段和方法,特别是在使用纤维素转化酶将纤维素转化成糖的那些情况下。 更具体而言,本发明涉及一种从木质纤维素材料生产可发酵糖的方法,其中木质纤维素材料与漆酶和能够降解纤维素的酶同时或以顺序推迟的方式接触,其中漆酶为 芽孢杆菌孢子外壳蛋白CotA。

    Gene Inactivated Mutants with Altered Protein Production
    8.
    发明公开
    Gene Inactivated Mutants with Altered Protein Production 有权
    基因失活的突变体与改变的蛋白质生产

    公开(公告)号:EP2333045A2

    公开(公告)日:2011-06-15

    申请号:EP10188499.7

    申请日:2006-04-11

    Inventor: Wang, Huaming

    Abstract: A recombinant filamentous fungal cell (eg. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to pepAb, derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.

    Abstract translation: 提供了具有一个或多个失活的染色体基因的重组丝状真菌细胞(例如曲霉属)。 在一些实施方案中,染色体基因对应于pepAb,derA,derB,htmA,mnn9,mnn10,ochA,dpp4,dpp5,pepAa,pepAc,pepAd,pepF及其组合。 重组真菌细胞可以包含进一步失活的染色体基因,其对应于pepA,pepB,pepC和pepD。 重组丝状真菌细胞可以包含编码感兴趣蛋白质的异源核酸。 还提供了在所述重组丝状真菌细胞中产生感兴趣的蛋白质的方法。

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