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128 条记录 (耗时 0.029 秒)

    METHOD FOR DETECTING NUCLEIC ACIDS
    81.
    发明公开
    METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
    检测方法的NUCLEIC的

    公开(公告)号：EP2430187A2

    公开(公告)日：2012-03-21

    申请号：EP10722154.1

    申请日：2010-05-11

    申请人： Abacus Diagnostica OY

    发明人： VON LODE, Piia ,  SYRJÄLÄ, Anniina ,  LÖVGREN, Timo ,  SOUKKA, Tero

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/689 ,  C12Q2537/161 ,  C12Q2527/107 ,  C12Q2525/204 ,  C12Q2565/107

    摘要： This invention relates to detecting nucleic acids. It employs a double-stranded oligonucleotide probe comprising i)a first probe comprising a first label moiety capable of emitting a measurable signal, and ii) a second probe being partially complementary with the first probe and comprising a second label moiety capable of interacting with the first moiety when brought in close proximity with each other, the second moiety being a quencher or acceptor of emission of the first moiety. The first or second probe comprises a sequence being complementary to that of a target nucleotide, and the second or first probe, respectively, comprises a sequence being complementary to a complement of the target nucleotide sequence of the nucleic acid to be detected. The first and the second moieties are attached to the first and second probes respectively in a manner wherein the distance between the first and second moieties is not more than 7 base pairs apart. The complementary sequences of the double-stranded probe is shorter than the full sequence of both the first and second single-stranded probes. The first and second probes have a higher T
    m when hybridized with the target nucleotide sequence compared to the T
    m of the double-stranded probe. The intensity of the signal of the first label when the first probe is not hybridized to the second probe is higher or lower than the intensity of the signal of the first label when the first probe is hybridized to the second probe. This invention also relates to oligonucleotides for determining Chlamydia trachomatis.

    Oligonucleotide hybridization method
    82.
    发明公开
    Oligonucleotide hybridization method 审中-公开
    Oligonukleotid-Hybridisierungsverfahren

    公开(公告)号：EP2270203A1

    公开(公告)日：2011-01-05

    申请号：EP09164052.4

    申请日：2009-06-29

    申请人： AIT Austrian Institute of Technology GmbH

    发明人： Krainer, Siegfried ,  Fluch, Silvia ,  Levente, Bodrossy ,  Stierschneider, Michael

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C12Q1/6837 ,  C12Q2537/161 ,  C12Q2527/101 ,  C12Q2525/107 ,  C12Q2527/107 ,  C12Q2525/121

    摘要： The present invention provides a method to bind oligonucleotide molecules of a sample to an oligonucleotide probe with increased specificity comprising the steps of
    • providing a solid support with at least one immobilized oligonucleotide DNA probe comprising an oligonucleotide probe sequence,
    • in any order, contacting said probes with a sample potentially comprising oligonucleotide molecules binding said probes and contacting said probes with at least one competitive non-DNA oligonucleotide, wherein said competitive oligonucleotide comprises a sequence which is complementary to the oligonucleotide probe sequence; and/or as an alternative or in addition, in any order, contacting said probes with a sample comprising oligonucleotide molecules and contacting said oligonucleotide molecules with at least one competitive non-DNA oligonucleotide, wherein said competitive oligonucleotide comprises a sequence of the oligonucleotide probe sequence,
    • incubating said contacted probes with the oligonucleotide molecules of the sample and competitive oligonucleotides at a temperature of between 15°C below the melting temperature of DNA duplex oligonucleotides of the probe sequence up to said melting temperature,

    thereby increasing binding specificity of the oligonucleotide probe to an oligonucleotide molecule comprising a sequence complementary to said probe sequence; as well as means for performing said method.

    摘要翻译： 本发明提供了将样品的寡核苷酸分子与特异性增加的寡核苷酸探针结合的方法，其包括以下步骤：向至少一种包含寡核苷酸探针序列的固定寡核苷酸DNA探针提供固体支持的步骤， 使所述探针与可能包含结合所述探针的寡核苷酸分子并使所述探针与至少一种竞争性非DNA寡核苷酸接触的样品接触，其中所述竞争性寡核苷酸包含与寡核苷酸探针序列互补的序列; 和/或作为替代或另外的任何顺序，使所述探针与包含寡核苷酸分子的样品接触并使所述寡核苷酸分子与至少一个竞争性非DNA寡核苷酸接触，其中所述竞争性寡核苷酸包含寡核苷酸探针序列 ，将所述接触的探针与样品的寡核苷酸分子和竞争性寡核苷酸在比探针序列的DNA双链寡核苷酸的解链温度低15℃的温度下培养直至所述解链温度，从而增加寡核苷酸的结合特异性 探针到包含与所述探针序列互补的序列的寡核苷酸分子; 以及用于执行所述方法的装置。

    COMPOSITIONS AND METHODS FOR THE IDENTIFICATION OF INHIBITORS OF RETROVIRAL INFECTION
    83.
    发明公开
    COMPOSITIONS AND METHODS FOR THE IDENTIFICATION OF INHIBITORS OF RETROVIRAL INFECTION 审中-公开
    组合物和用于鉴定病毒感染复古抑制剂

    公开(公告)号：EP2197455A2

    公开(公告)日：2010-06-23

    申请号：EP08832718.4

    申请日：2008-09-12

    申请人： North Carolina State University

    发明人： AGRIS, Paul F. ,  GUENTHER, Richard H.

    IPC分类号： A61K31/7105 ,  C12Q1/68

    CPC分类号： C12Q1/703 ,  C12N9/99 ,  C12N2740/10011 ,  C12N2740/16011 ,  C12Q2600/136 ,  C12Q2537/161 ,  C12Q2527/127 ,  C12Q2521/107

    摘要： Methods of identifying inhibitors of retroviral propagation, tRNA used in the methods, and kits, including the tRNA, which can be used in the methods, are disclosed. Methods of treating or preventing retroviral infections by administering an effective amount of the inhibitors, and pharmaceutical compositions including the inhibitors, are also disclosed. The methods involve forming a mixture comprising a linear sequence of a tRNA anticodon stem loop fragment that is not capable of forming a stem-loop, a target nucleic acid molecule capable of binding to the tRNA anticodon stem loop fragment, and a test compound. The mixture is incubated under conditions that allow binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule in the absence of the test compound. Assays can then be performed that detect whether or not the test compound inhibits the binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule.

    Fluorescence energy transfer by competitive hybridization
    86.
    发明公开
    Fluorescence energy transfer by competitive hybridization 失效
    荧光素能量转移

    公开(公告)号：EP1666609A1

    公开(公告)日：2006-06-07

    申请号：EP06110485.7

    申请日：1998-03-02

    申请人： Quest Diagnostics Investments Incorporated

    发明人： Chiang, Chih-Sheng ,  Cuan, Jose F.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6818 ,  C12Q2537/161 ,  C12Q2525/204

    摘要： A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorphore.

    摘要翻译： 提供了用于检测核苷酸的存在或监测核苷酸扩增的方法。 它通过竞争性杂交利用荧光能量转移。 通过使用在一个探针上具有荧光团和另一个探针上的猝灭剂的不等长的互补探针来实现竞争性杂交。 荧光团和猝灭剂以其中猝灭剂与荧光团的接近度产生荧光荧光的猝灭的方式并置。

    Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates and enzyme inhibition assays
    87.
    发明公开
    Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates and enzyme inhibition assays 失效
    Erstärkungder alkalischen Phosphatase durch SDSds als Chemilumineszsubstrat und Verfahren zur Enzyminhibition

    公开(公告)号：EP1616966A2

    公开(公告)日：2006-01-18

    申请号：EP05018281.5

    申请日：1996-06-07

    申请人： BAYER CORPORATION

    发明人： Sheriden, Patrick J. ,  Gagne, Julio C. ,  Anderson, Mary L. ,  Ludtke, Douglas N.

    IPC分类号： C12Q1/68 ,  G01N33/58

    CPC分类号： G01N33/581 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/582 ,  G01N2333/916 ,  C12Q2563/125 ,  C12Q2527/125 ,  C12Q2525/197 ,  C12Q2537/161 ,  C12Q2537/125

    摘要： The invention relates to a method of detecting a target oligonucleotide in a sample, comprising: (a) providing a support-bound capture probe (CP) comprising a region having a nucleic acid sequence C-3; (b) providing a label probe (LP) comprising a region having a nucleic acid sequence L-3, wherein the label probe contains a label moiety that is capable of generating a detectable signal; (c) providing a label extender (LE) comprising a region having first and second nucleic acid sequences, wherein the first LE nucleic acid sequences L-2 is complementary to nucleic acid sequences L-3 and the second LE nucleic acid sequence L-1 is complementary to a nucleic acid sequence in the target analyte; (d) providing a capture extender (CE) comprising a region having first and second nucleic acid sequences, wherein the first CE nucleic acid sequences C-2 is complementary to nucleic acid sequence C-3 and the second CE nucleic acid sequence C-1 is complementary to the second LE nucleic acid sequence L-1; (e) incubating the sample with the LE and the LP under first hybridizing conditions to form a first reaction mixture containing an LP/LE/target hybrid complex and an unbound LP/LE hybrid complex; (f) incubating the first reaction mixture with a solid support surface containing a surface-bound CE/CP hybrid complex under second hybridizing conditions, thereby forming a second reaction mixture containing a surface-bound LP/LE/target/CE/CP hybrid complex and an unbound LP/LE/target complex; (g) thereafter separating materials not bound to the solid support from those bound to the solid support; and (h) detecting the presence of label in the support-bound, LP/LE/target/CE/CP hybrid complex.

    摘要翻译： 本发明涉及一种检测样品中靶寡核苷酸的方法，包括：（a）提供包含具有核酸序列C-3的区域的载体结合捕获探针（CP）; （b）提供包含具有核酸序列L-3的区域的标记探针（LP），其中所述标记探针含有能够产生可检测信号的标记部分; （c）提供包含具有第一和第二核酸序列的区域的标签扩增物（LE），其中第一LE核酸序列L-2与核酸序列L-3和第二LE核酸序列L-1互补 与靶分析物中的核酸序列互补; （d）提供包含具有第一和第二核酸序列的区域的捕获扩增体（CE），其中第一CE核酸序列C-2与核酸序列C-3和第二CE核酸序列C-1互补 与第二LE核酸序列L-1互补; （e）在第一杂交条件下将样品与LE和LP孵育以形成含有LP / LE /靶混合复合物和未结合的LP / LE杂交复合物的第一反应混合物; （f）在第二杂交条件下将第一反应混合物与包含表面结合的CE / CP杂化复合物的固体支持物表面孵育，从而形成含有表面结合的LP / LE /靶/ CE / CP杂化复合物的第二反应混合物 和一个未绑定的LP / LE /目标复合体; （g）此后分离与固体支持物结合的物质不结合到固体支持物上; 和（h）检测载体结合的LP / LE /靶/ CE / CP杂合体中标记的存在。