摘要:
Provided herein is a method of purification of non-enveloped or pseudo-enveloped virus produced in vitro using a composition with at least one detergent. Also provided are a method of purification can use multiple detergents, and a method of determining the presence and/or level of a non-enveloped or pseudo-enveloped virus in a sample.
摘要:
The present invention belongs to the field of in-vitro diagnostics. Within this field, it particularly concerns the amplification of at least a first target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. It further provides an analytical system comprising an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer.
摘要:
There is described a method for detecting a hepatitis A virus (HAV) target nucleic acid in a sample, said method comprising: (A) providing a sample suspected of containing HAV; (B) contacting said sample with an oligomer combination for amplifying a HAV nucleic acid target region, said oligomer combination comprising: (a) a first HAV amplification oligomer comprising a first target-hybridizing sequence that is from about 14 to about 27 contiguous nucleotides contained in the sequence of SEQ ID NO:174 and that includes at least the sequence of SEQ ID NO:173; and (b) a second HAV amplification oligomer comprising a second target-hybridizing sequence that is from about 14 to about 30 contiguous nucleotides contained in the sequence of SEQ ID NO:177 and that includes at least the sequence of SEQ ID NO:175; (C) performing an in vitro nucleic acid amplification reaction, wherein any HAV target nucleic acid present in said sample is used as a template for generating a HAV amplification product; and (D) detecting the presence or absence of the HAV amplification product, thereby indicating the presence or absence of HAV in said sample.
摘要翻译:本发明描述了用于检测A型肝炎病毒(HAV)靶核酸的样品中,所述方法包括以下步骤:(A)提供怀疑含有HAV的样品; (B)使所述样品与低聚物组合用于扩增HAV核酸靶区域,所述低聚物组合物,包括:(a)第一HAV扩增寡聚体,其包括第一靶标杂交序列并从约14至约27个连续的核苷酸包含 在SEQ ID NO:1的序列:174,并且包括至少做了SEQ ID NO:1的序列:173; 和(b)第二HAV扩增寡聚体包括第二靶标杂交序列所做的是从约14至包含SEQ ID NO:1的序列中约30个连续核苷酸:177,做了至少包括SEQ ID NO:1的序列:175; (C)进行体外核酸扩增反应,worin任何HAV目标存在于所述样品中的核酸被用作用于生成HAV扩增产物的模板; 和(d)检测的HAV扩增产物的存在或不存在,从而指示所述样品中存在或不存在的HAV。
摘要:
The present invention provides a rapid and highly effective method for the preparation of biological samples for detection of both DNA and RNA target molecules. The method comprises treatment of the sample with a clay mineral followed by lysis of the sample with an alkaline solution. The method is particularly suited for preparing complex biological samples, such as blood or plasma, for the simultaneous detection of DNA and RNA targets. Samples prepared by the method of the invention may be directly used as targets in PCR amplification. The method of the invention may conveniently be coupled with further steps and devices to perform molecular analyses for diagnostic and other applications.