公开(公告)号：EP1846748A4

公开(公告)日：2009-05-20

申请号：EP06720022

申请日：2006-02-01

申请人： ARRYX INC

发明人： MUETH DANIEL ,  ANDERSON AMY L ,  KNUTSON CHRISTOPHER R ,  PLEWA JOSEPH

IPC分类号： G01N21/01 ,  G01N33/00

CPC分类号： G01N21/6428 ,  C12M47/04 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/149

摘要： An apparatus for sorting and orienting sperm cells includes a pair of walls in confronting arrangement and forming a flow chamber having an inlet, a downstream outlet, and an intermediate detector region. The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid containing the cells to be sorted. The first and second streams have respective flow rates relative to the third stream, such that the third stream is constricted, forming a relatively narrow sample stream, so that the cells are oriented parallel to the walls. A detector detects the desired cells and a sorter downstream of the detector, sorts the desired cells from the stream.

公开(公告)号：EP2053380A2

公开(公告)日：2009-04-29

申请号：EP08167249.5

申请日：2008-10-22

申请人： Becton, Dickinson and Company

发明人： Schembri, Jr., Frank ,  Vrane, David

IPC分类号： G01N15/14

CPC分类号： G01N15/1459 ,  G01N2015/1409 ,  G01N2015/149

摘要： The present invention provides deflection plates for use in a flow-type particle sorter that arc resistant to wetting. The deflection plates include a gas-porous, conductive plate. A gas, such as air, is passed through the plate from the outer face (away from the particle flow) towards the inner face (towards the particle flow). The flow of gas prevents condensation on the inner face of the defection plate.

摘要翻译： 本发明提供了用于流动型颗粒分选机的偏转板，该偏转板具有抗潮湿的抗电弧性能。 偏转板包括气体导电板。 诸如空气的气体从外表面（远离颗粒流）朝向内表面（朝向颗粒流）穿过板。 气流阻止防漏板内表面的冷凝。

公开(公告)号：EP1281059B1

公开(公告)日：2008-12-17

申请号：EP01913732.2

申请日：2001-03-09

申请人： Inverness Medical Switzerland GmbH

发明人： LARSEN, Ulrik, Darling ,  WOLFF, Anders ,  TELLEMAN, Pieter

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N15/14 ,  G01N30/6095 ,  G01N2015/1409 ,  G01N2015/1413 ,  Y10T137/0363 ,  Y10T137/87571 ,  Y10T137/87587 ,  Y10T137/87595

摘要： With a method of establishing an enveloped flow in a channel, in the channel an envelopment area ( 3 ) with a channel section ( 6, 7 ) is configured to which a stub with an outlet is connected, where the stub, which can be configured as a polyhedron, is arranged substantially at right-angles to the envelopment area and with the plane of the outlet substantially at right-angles to the channel section. An inlet channel ( 9, 11 ) and an outlet channel ( 8, 10 ) are connected to the envelopment area. By providing a narrowing-down in the cross-section of the channel in immediate extension of the envelopment area ( 3 ), a hydrodynamic focussing of the sample fluid is achieved when a fluid, such as a sample fluid, is introduced into the stub, and a carrier fluid is introduced into the inlet channel. The advantage of the invention is that since it is necessary only to have two fluid inlets, a relatively simple structure can be established, which can be built up in a monolithic manner, e.g. by injection molding.

公开(公告)号：EP1921438A2

公开(公告)日：2008-05-14

申请号：EP07253990.1

申请日：2007-10-09

申请人： Genetix Limited

发明人： Jiang, Yonggang

IPC分类号： G01N15/10

CPC分类号： G01N15/1012 ,  G01N2015/1409 ,  G01N2015/1415 ,  Y10T436/10 ,  Y10T436/2575

摘要： A calibration method for a flow cytometer with a multichannel detector module. During calibration, the fluorescence intensity data values for the different detector channels are used to calculate normalization factors needed to adjust subsequent data collected by each of the channels. By using a multichannel detector module, the results from the different flow cells can be reliably compared, so that multiple stages of flow cells can be arranged in series along a common flow path, for example to measure the same sample at defined time intervals.

摘要翻译： 具有多通道检测器模块的流式细胞仪的校准方法。 在校准期间，使用不同检测器通道的荧光强度数据值来计算调整由每个通道收集的随后数据所需的归一化因子。 通过使用多通道检测器模块，可以可靠地比较来自不同流动池的结果，使得可以沿着公共流动路径串联布置多个流动池，例如以限定的时间间隔测量相同的样品。

公开(公告)号：EP1835276A1

公开(公告)日：2007-09-19

申请号：EP06111193.6

申请日：2006-03-15

申请人： FOSS Analytical A/S

发明人： Thomsen, Henrik FOSS Analytical A/S ,  Skærlund Frandsen, Anders FOSS Analytical A/S ,  Born, Christian FOSS Analytical A/S ,  Brix Larsen, Ole FOSS Analytical A/S ,  Holm, Claus FOSS Analytical A/S

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N35/1095 ,  G01N2015/1409

摘要： An apparatus, such as a flow cytometer, for determining compositional properties of a material (38), comprises sensing means (24) having an output responsive to components of interest within a sample, here liquid (38), at a measurement location (22). A flow regulator, such as a pump (36), is provided in flow communication with the measurement location (22) and with the liquid sample (38). Control means (34) is adapted to analyse, such as by employment of an pulse height analyser (32), the output to determine a contribution thereto by noise and to control the operation of the flow regulator (36) to regulate presentation of the sample, here the flow rate, at the measurement location (22) in a manner dependent on the determined noise contribution.

摘要翻译： 用于确定材料（38）的组成性质的装置，例如流式细胞仪，包括感测装置（24），其具有响应于在测量位置（22）处的样品（在此为液体）（38）内的感兴趣组分的输出 ）。 提供流量调节器，例如泵（36），与测量位置（22）和液体样品（38）流动连通。 控制装置（34）适于例如通过使用脉冲高度分析器（32）来分析输出以确定噪声对其的贡献，并且控制流量调节器（36）的操作以调节样品的显示 ，这里是以取决于所确定的噪声贡献的方式在测量位置（22）处的流量。

公开(公告)号：EP1834167A2

公开(公告)日：2007-09-19

申请号：EP05852982.7

申请日：2005-12-02

申请人： XY, Inc. ,  Chata Biosystems, Inc.

发明人： NEAS, Edwin, Dean ,  KUIKEN, Jerald, Edward ,  SCHENK, John, Louis ,  GILLIGAN, Thomas, Boyd

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N15/14

CPC分类号： G01N15/1484 ,  B01L3/0296 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2400/0481 ,  G01N35/1095 ,  G01N2015/1409 ,  G01N2015/1481 ,  G01N2015/149 ,  Y10T137/0318 ,  Y10T137/1624 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

摘要： A fluid handling and delivery system useful in generating a fluid stream (7) in the flow path (8) of microfluidic device (16).

公开(公告)号：EP1664738A1

公开(公告)日：2006-06-07

申请号：EP04764723.5

申请日：2004-09-02

申请人： HYDAC FILTERTECHNIK GMBH

发明人： KLEBER, Jörg ,  BUSCH, Andreas

IPC分类号： G01N15/14 ,  G01N15/12

CPC分类号： G01N35/1095 ,  G01N3/567 ,  G01N15/06 ,  G01N15/10 ,  G01N15/12 ,  G01N15/1404 ,  G01N33/2888 ,  G01N2015/1062 ,  G01N2015/1409

摘要： The invention relates to a device with a particle counter (22), wherein an evaluatable sensor signal is produced by means of a sensor which reacts to the presence of particles (14) in a measuring area through which a viscous medium (12) fluid flows. Before the fluid flow enters the sensor measuring zone of the particle counter (22), the input pressure can be adapted for the viscous medium (12) by means of an adjustment device (28). By virtue of the fact that the adjustment device, which is used to adapt the input pressure to temperature-dependent viscosity of the medium (12), is arranged on the off-flow side (24) of the sensor and/or in a secondary branch (34) thereto, it is possible to produce an input pressure, which is suitable for particle measurement according to the viscosity of the fluid (medium), on said particle counter using the sensor.

公开(公告)号：EP1179087A4

公开(公告)日：2005-12-21

申请号：EP00932435

申请日：2000-05-11

申请人： CALIPER LIFE SCIENCES INC

发明人： WADA H GARRETT ,  KOPF-SILL ANNE R ,  ALAJOKI MARJA LIISA ,  PARCE J WALLACE ,  WANG BENJAMIN N ,  CHOW ANDREA W ,  DUBROW ROBERT S

IPC分类号： B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  G01N1/40 ,  G01N15/14 ,  G01N27/447 ,  C12Q1/00

CPC分类号： B01F13/0062 ,  B01J19/0093 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2400/0406 ,  B01L2400/0487 ,  G01N15/1404 ,  G01N27/44704 ,  G01N27/44791 ,  G01N2015/1409 ,  G01N2015/149 ,  G01N2333/4718 ,  Y10T436/117497 ,  Y10T436/2575

摘要： Methods and systems for particle focusing to increase assay throughput in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.

公开(公告)号：EP1521076A1

公开(公告)日：2005-04-06

申请号：EP04023071.6

申请日：2004-09-28

申请人： Sysmex Corporation

发明人： Jindo, Katsuhiko

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  F04B11/0075 ,  F04B53/16 ,  G01N2015/1409 ,  G01N2015/1411

摘要： The sheath flow forming device are described that include: a container for storing sample fluid and having a supply port for supplying the sample fluid; a second container for storing sheath fluid and having a supply port for supplying the sheath fluid; a flow cell having a sample fluid inlet for receiving sample fluid supplied from the supply port of the first container, a sheath fluid inlet for receiving the sheath fluid from the supply port of the second container, and an outlet for discharging the mixture of the sheath fluid and the sample fluid; a first pump for supplying a sheath fluid to the sheath fluid inlet; a second pump for suctioning fluid within the flow cell through the outlet of the flow cell; and a first drive source for driving the first pump and second pump.

摘要翻译： 鞘流形成装置被描述并包括：用于存储样品流体且具有用于供给样品流体的供给口的容器; 用于存储鞘流体且具有用于供给鞘液的供给口的第二容器; 具有用于接收来自所述第一容器，用于接收鞘液从第二容器的供给口的鞘流体入口的供给口供给样品液的样品流体入口的流动池，并且在出口用于排出所述护套的混合物 流体和样品流体; 第一泵供给鞘液鞘液入口; 用于抽吸通过所述流动池的出口处的流动单元内流体的第二泵; 以及用于驱动所述第一泵和第二泵中的第一驱动源。

公开(公告)号：EP1391611A2

公开(公告)日：2004-02-25

申请号：EP03016774.6

申请日：2003-07-23

申请人： Becton, Dickinson and Company

发明人： Vrane, David R. ,  Pierce O. Norton

IPC分类号： F04B11/00 ,  F04B23/02

CPC分类号： G05D16/2066 ,  F04B11/0025 ,  G01N15/1404 ,  G01N35/1095 ,  G01N2015/1409 ,  Y10T137/3118 ,  Y10T137/3124

摘要： A device for conditioning of fluid to minimize pulses from a pump source. A pump drives fluid from a vented fluid reservoir through a system including a combination of an attenuation tank and one or more orifice. Fluid is pumped into a plenum chamber that supplies fluid in a pulse free flow stream. A pressurized head over the fluid in the plenum chamber allows fluid delivery at a selected pressure. A volume sensor monitors the fluid level within the plenum tank and activates the pump if fluid level falls below a specified level.

摘要翻译： 用于调节流体以最小化来自泵浦源的脉冲的装置。 泵通过包括衰减箱和一个或多个孔的组合的系统驱动来自排放的流体储存器的流体。 将流体泵送到以无脉冲流动流提供流体的增压室。 在增压室中的流体上的加压头允许在选定压力下的流体输送。 体积传感器监测增压室内的液位，如果液位低于指定水平，则启动泵。