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81.发明公开Method and reagent for quantitative determination of 1,5-anhydroglucitol 审中-公开Verfahren und Reagenz zur quantitativen Bestimmung von 1,5-Anhydroglucitol
公开(公告)号：EP1008657A2
公开(公告)日：2000-06-14
申请号：EP99309932.4
申请日：1999-12-10
CPC分类号： C12Q1/32 , C12Q1/54 , Y10S435/966 , Y10S435/975
摘要： The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1, 5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要翻译： 本发明提供了一种用于确定特定成分的简单方法，例如， 含有葡萄糖的样品中的1,5-脱水葡萄糖醇（1,5-AG）和可用于该方法的试剂和试剂盒。 在一个实施方案中，提供了用于测定1,5-AG的方法，其包括使样品与将葡萄糖转化为果糖-1,6-二磷酸并将1,5-AG转化为1,5-AG的酶系统接触 -6-磷酸形成1,5-AG-6-磷酸，在1,5-AG-6-磷酸脱氢酶的作用下，在氧化型辅酶存在下，使样品中的1,5-AG-6-磷酸脱氢 ，并测量由脱氢反应形成的还原型辅酶的量。 还提供了一种在该方法中有用的试剂和试剂盒。
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公开(公告)号：EP0937779A3
公开(公告)日：2000-05-17
申请号：EP99102683.2
申请日：1999-02-12
发明人： Yamori, Yukio , Ikeda, Katsumi c/o Hagisaki-manshion 303 , Uchida, Kohji , Taniguchi, Yoshiyuki , Matuo, Yuhsi
摘要： The concentration of phosphoglyceric acid mutase (PGAM) in a cell culture is assayed by a reagent containing enolase, pyruvate kinase, NADH, and lactate dehydrogenase. PGAM is useful as a damage marker of various cells or a cell toxicity marker thereof.
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公开(公告)号：EP0835938A3
公开(公告)日：2000-01-19
申请号：EP97307937.9
申请日：1997-10-08
发明人： Gwynn, Michael N., SmithKline Beecham Pharma , Kallendar, Howard, SmithKline Beecham Pharma , Palmer, Leslie M., SmithKline Beecham Pharma
摘要： Topoisomerase I polypeptides and DNA and RNA encoding such Topoisomerase I polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such Topoisomerase I for the treatment of infection, particularly bacterial infections. Antagonists against such Topoisomerase I and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of Topoisomerase I nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding Staphylococcal Topoisomerase I and for detecting the polypeptide in a host.
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公开(公告)号：EP0837137A3
公开(公告)日：2000-01-12
申请号：EP97308158.1
申请日：1997-10-15
摘要： Topoisomerase III polypeptides and DNA and RNA encoding such Topoisomerase III polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such Topoisomerase III for the treatment of infection, particularly bacterial infections. Antagonists against such Topoisomerase III and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of Topoisomerase III nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding Streptococcal Topoisomerase III and for detecting the polypeptide in a host.
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85.发明公开Staphylococcus aureus member of the DEAD-type ATP-dependent RNA helicases (dbpB) 审中-公开的DEAD型ATP依赖的RNA解旋酶的金黄色葡萄球菌构件（dbpB）
公开(公告)号：EP0913481A3
公开(公告)日：1999-12-29
申请号：EP98203442.3
申请日：1998-10-12
发明人： Huang, Jianzhong, Smithkline Beecham Pharmaceutic. , McDevitt, Damien, Smithkline Beecham Pharmaceutic. , Wang, Min, Smithkline Beecham Pharmaceuticals , Traini, Christopher M., Smithkline Beech. Pharmac.
摘要： The invention provides dbpB polypeptides and DNA (RNA) encoding dbpB polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing dbpB polypeptides to screen for antibacterial compounds.
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公开(公告)号：EP3322417B1
公开(公告)日：2023-08-23
申请号：EP16824891.2
申请日：2016-07-05
IPC分类号： A61K31/427 , A61K31/444 , A61K31/454 , A61K31/138 , A61K31/277 , A61K31/4515 , A61K31/4545 , A61K31/496 , A61K31/5355 , A61K31/5377 , A61K31/55 , C12Q1/533 , G01N33/50 , A61K31/58 , A61P35/00
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公开(公告)号：EP3999849A1
公开(公告)日：2022-05-25
申请号：EP20841288.2
申请日：2020-07-15
申请人： Youcount Inc. , Dechev, Teodora , Dechev, Nikolai , Fraser, Ian
发明人： DECHEV, Teodora , DECHEV, Nikolai , FRASER, Ian
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88.发明公开
公开(公告)号：EP3623480A1
公开(公告)日：2020-03-18
申请号：EP18799253.2
申请日：2018-05-11
发明人： KIM, Seon Won , HAN, Hyo Jung , KIM, Sin Young , PARK, Ji Bin
摘要： The present invention provides a marker composition for selecting a living modified organism, which allows transformation and the production of a target product without antibiotics or antibiotic resistance genes, a living modified organism, a method of transforming an organism, and a method of producing a target product. The present invention provides a marker composition for selecting a living modified organism, which may basically prevent problems caused by the use of antibiotics and antibiotic resistance genes and produce a target product at a high yield, a living modified organism, a method of transforming an organism, and a method of producing a target product.
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89.发明公开METHODS OF IDENTIFYING ENZYMES AND MICROORGANISMS 审中-公开密克罗尼西亚VERFAHREN ZUR IDENTIFIZIERUNG VON ENZYMEN
公开(公告)号：EP3143157A1
公开(公告)日：2017-03-22
申请号：EP15723908.8
申请日：2015-05-15
申请人： Zymonostics ApS
发明人： STOUGAARD, Magnus , KNUDSEN, Birgitta Ruth , HEDE, Marianne Smedegaard , THOMSEN, Jonas , HO, Yi-Ping
CPC分类号： C12Q1/6893 , C12Q1/025 , C12Q1/04 , C12Q1/25 , C12Q1/485 , C12Q1/533 , C12Q1/68 , C12Q1/689 , G01N33/573 , G01N2333/9015 , G01N2333/91245 , G01N2800/26 , Y02A50/58 , C12Q2521/501 , C12Q2533/107 , C12Q2565/629 , C12Q2521/507 , C12Q2521/519
摘要： The invention provides a method of identifying a microorganism that expresses a nucleic acid-modifying enzyme, in sample, the method comprising: (a) contacting a nucleic acid substrate targeted by the nucleic acid-modifying enzyme with the sample; (b) adding a further nucleic acid molecule to the sample which nucleic acid molecule is ligated to the nucleic acid substrate in the presence of the nucleic acid-modifying enzyme to form a ligation product which comprises a linear single strand of nucleic acid that is capable of being detected; and (c) detecting the presence of the ligation product.
摘要翻译： 本发明提供了一种鉴定在样品中表达核酸修饰酶的微生物的方法，所述方法包括：（a）使核酸修饰酶靶向的核酸底物与样品接触; （b）在所述样品中加入另外的核酸分子，所述核酸分子在所述核酸修饰酶存在下与所述核酸底物连接以形成连接产物，所述连接产物包含能够具有的线性单链核酸 被检测到 和（c）检测结扎产物的存在。
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90.发明公开FERTILITY TEST METHOD, FERTILITY TEST KIT, POLYNUCLEOTIDE, POLYPEPTIDE AND ANTIBODY 有权生育力测试过程中，FRUCHTBARKEITSTESTKIT，多核苷酸，多肽抗体
公开(公告)号：EP2410050A4
公开(公告)日：2013-02-20
申请号：EP10753285
申请日：2010-03-15
CPC分类号： G01N33/689 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , G01N2333/99
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