-
1.发明公开
公开(公告)号：EP1572912A2
公开(公告)日：2005-09-14
申请号：EP03716849.9
申请日：2003-03-27
申请人： Gala Design, Inc.
IPC分类号： C12N1/00
CPC分类号： C12N15/86 , C12N2510/02 , C12N2740/13043 , C12N2740/15043 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene. The present invention further relates to the use of integrating vectors lacking a selectable marker and growth of host cells containing such vectors in the absence of selection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.
-
公开(公告)号：EP1495146B1
公开(公告)日：2008-11-19
申请号：EP03745659.7
申请日：2003-03-28
申请人： Gala Design, Inc.
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
-
公开(公告)号：EP1495146A2
公开(公告)日：2005-01-12
申请号：EP03745659.7
申请日：2003-03-28
申请人： Gala Design, Inc.
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
摘要： The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.
-
公开(公告)号：EP1297168A2
公开(公告)日：2003-04-02
申请号：EP01948822.0
申请日：2001-06-29
申请人： Gala Design, Inc.
发明人： Bleck, Gregory, T.
CPC分类号： C12N15/86 , A61K48/00 , C07K16/1228 , C07K16/1282 , C07K16/3007 , C07K16/3061 , C12N2740/13043 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203
摘要： The present invention provides novel regulatory elements and vectors for the expression of one or more proteins in a host cell. The present invention also provides methods for espressing on or more proteins, such as antibodies, in a host cell. These methods utilize the novel regulatory elements and vectors of the present invention for the expression of proteins in a host cell. The host cells are used for producing various protein products, including but not limited to pharmaceutical proteins, antibodies, variants of proteins for use in screening assays, and for direct use in high throughput screening.
-
5.发明公开PRODUCTION OF HOST CELLS CONTAINING MUTIPLE INTEGRATING VECTORS BY SERIAL TRANSDUCTION 审中-公开宿主细胞多个生产整合载体包含由串行转导
公开(公告)号：EP1751282A2
公开(公告)日：2007-02-14
申请号：EP05705678.0
申请日：2005-01-14
申请人： Gala Design, Inc.
IPC分类号： C12N15/09
CPC分类号： C12P21/02 , C07K14/005 , C07K14/76 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C07K2319/02 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N15/85 , C12N15/86 , C12N2510/02 , C12N2740/13022 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2760/16122 , C12N2830/00 , C12N2830/008 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12N2840/85
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.
-
公开(公告)号：EP1495043A4
公开(公告)日：2006-07-12
申请号：EP03723845
申请日：2003-03-27
申请人： GALA DESIGN INC
发明人： BREMEL ROBERT D , BLECK GREGORY T , IMBODEN MICHAEL , EAKLE KURT
IPC分类号： C12N5/10 , C12N15/10 , C12N15/867 , C07K1/26 , C07H21/04 , C12N15/63 , C12N15/85 , C12P19/34 , C12Q1/70
CPC分类号： C12N15/1082 , C12N7/00 , C12N15/86 , C12N2740/13043 , C12N2740/13052 , C12N2830/42 , C12N2830/48 , C12N2840/203
摘要： The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5' and 3' viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.
-
公开(公告)号：EP1299556A2
公开(公告)日：2003-04-09
申请号：EP01950645.0
申请日：2001-06-29
申请人： Gala Design, Inc.
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N15/85 , C12N2730/10122 , C12N2740/13043 , C12N2740/13045 , C12N2740/13062 , C12N2750/14143 , C12N2810/6081 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
-
8.发明公开
公开(公告)号：EP1572912A4
公开(公告)日：2007-09-19
申请号：EP03716849
申请日：2003-03-27
申请人： GALA DESIGN INC
发明人： BREMEL ROBERT D , YORK DONA , BLECK GREGORY T , EAKLE KURT A
IPC分类号： C12N15/867 , C12P21/02 , C12N15/09 , C12N5/10 , C12N15/63
CPC分类号： C12N15/86 , C12N2510/02 , C12N2740/13043 , C12N2740/15043 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
-
9.发明公开Host cells containing multiple integrating vectors 有权Wirtzellen mit mehreren integrierenden Vektoren
公开(公告)号：EP2151493A1
公开(公告)日：2010-02-10
申请号：EP09014803.2
申请日：2001-06-29
申请人： Gala Design, Inc.
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N15/85 , C12N2730/10122 , C12N2740/13043 , C12N2740/13045 , C12N2740/13062 , C12N2750/14143 , C12N2810/6081 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译： 本发明涉及在宿主细胞中产生蛋白质，更具体地说涉及含有多个整合载体的集成拷贝的宿主细胞。 用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。 提供了通过使用高感染多重性的整合载体制备宿主细胞的方法。 宿主细胞可用于产生药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。
-
10.发明公开PRODUCTION OF HOST CELLS CONTAINING MUTIPLE INTEGRATING VECTORS BY SERIAL TRANSDUCTION 审中-公开宿主细胞多个生产整合载体包含由串行转导
公开(公告)号：EP1751282A4
公开(公告)日：2009-08-26
申请号：EP05705678
申请日：2005-01-14
申请人： GALA DESIGN INC
发明人： BLECK GREGORY T , BREMEL ROBERT D , MILLER LINDA U
IPC分类号： C12N15/867 , C07K14/02 , C07K14/76 , C07K16/12 , C07K16/30 , C12N5/10 , C12N7/04 , C12N15/82 , C12N15/85 , C12P21/02
CPC分类号： C12P21/02 , C07K14/005 , C07K14/76 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C07K2319/02 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N15/85 , C12N15/86 , C12N2510/02 , C12N2740/13022 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2760/16122 , C12N2830/00 , C12N2830/008 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12N2840/85
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.
摘要翻译： 本发明涉及在宿主细胞中生产的蛋白，更具体地说，涉及主机包含一个INTEGRA婷载体，其包含外源基因，并通过串行转导或转染制备这样的宿主细胞的方法的多个整合拷贝的细胞。 本发明进一步提供了在使用搜索载体的宿主细胞中表达的蛋白的水平增加的方法。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.021 秒)
