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    Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution
    2.
    发明公开
    Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution 失效
    在水溶液中未保护的或n端半胱氨酸保护的肽的固相天然化学连接

    公开(公告)号：EP1518862A2

    公开(公告)日：2005-03-30

    申请号：EP04029270.8

    申请日：1998-06-12

    申请人： Gryphon Therapeutics, Inc.

    发明人： Canne, Lynne ,  Kent, Stephen B.H. ,  Simon, Reyna

    IPC分类号： C07K1/02 ,  C07K1/04 ,  C07K1/08

    CPC分类号： C07K1/023 ,  C07K1/026 ,  C07K1/04 ,  C07K1/047 ,  C07K1/086

    摘要： A method of preparing an assembled peptide comprising:

    a) binding a first peptide segment to a solid phase via a linker to form a solid phase-bound peptide;
    b) ligating at least a second peptide segment to said solid phase-bound peptide in aqueous solution to form a solid phase-bound peptide. Preferably the first and second peptide segments are unprotected, except that if the second peptide segment has an N-terminal cysteine, it is optionally protected.

    摘要翻译： 制备组装肽的方法，其包括：a）通过接头将第一肽片段结合至固相以形成固相结合肽; b）在水溶液中将至少第二肽片段连接至所述固相结合肽以形成固相结合肽。 优选地，第一和第二肽片段是未保护的，除了如果第二肽片段具有N-末端半胱氨酸，则其任选地被保护。

    DISPLACEABLE LINKER SOLID PHASE CHEMICAL LIGATION
    6.
    发明公开
    DISPLACEABLE LINKER SOLID PHASE CHEMICAL LIGATION 审中-公开
    化学固相结扎互换LEFT

    公开(公告)号：EP1631597A4

    公开(公告)日：2007-07-11

    申请号：EP04752449

    申请日：2004-05-17

    申请人： GRYPHON THERAPEUTICS INC

    发明人： LOW DONALD ,  HU YING ,  BOTTI PAOLO

    IPC分类号： C08F2/00 ,  A61K20060101 ,  C07K1/04 ,  C07K1/107 ,  C08B37/00

    CPC分类号： C07K1/042 ,  C07K1/1072

    摘要： The invention is directed to methods and compositions for aqueous-compatible solid phase chemical ligation. The methods and compositions of the invention involve the chemical ligation of first and second polymers, where the first polymer is attached to a support through a displaceable linker. The displaceable linker can be cleaved or otherwise displaced under aqueous conditions compatible with subsequent removal of the ligation product from the solid support. The methods and compositions of the invention are particularly useful for ligation of peptides and polypeptides on a solid support. The ligation system of the invention is applicable to a wide variety of molecules, and thus can be exploited to generate ligated polymers including peptides, polypeptides and other amino acid containing polymers.

    DISPLACEABLE LINKER SOLID PHASE CHEMICAL LIGATION
    8.
    发明公开
    DISPLACEABLE LINKER SOLID PHASE CHEMICAL LIGATION 审中-公开
    化学固相结扎互换LEFT

    公开(公告)号：EP1631597A2

    公开(公告)日：2006-03-08

    申请号：EP04752449.1

    申请日：2004-05-17

    申请人： Gryphon Therapeutics, Inc.

    发明人： LOW, Donald ,  HU, Ying ,  BOTTI, Paolo

    IPC分类号： C08F2/00 ,  C08B37/00

    CPC分类号： C07K1/042 ,  C07K1/1072

    摘要： The invention is directed to methods and compositions for aqueous-compatible solid phase chemical ligation. The methods and compositions of the invention involve the chemical ligation of first and second polymers, where the first polymer is attached to a support through a displaceable linker. The displaceable linker can be cleaved or otherwise displaced under aqueous conditions compatible with subsequent removal of the ligation product from the solid support. The methods and compositions of the invention are particularly useful for ligation of peptides and polypeptides on a solid support. The ligation system of the invention is applicable to a wide variety of molecules, and thus can be exploited to generate ligated polymers including peptides, polypeptides and other amino acid containing polymers.

    MULTIPLEX POLYMER LIGATION
    9.
    发明公开
    MULTIPLEX POLYMER LIGATION 审中-公开
    多重POLYMERLIGATION

    公开(公告)号：EP1616021A2

    公开(公告)日：2006-01-18

    申请号：EP03814988.6

    申请日：2003-12-30

    申请人： Gryphon Therapeutics, Inc.

    发明人： KOCHENDOERFER, Gerd, G. ,  SHAO, Haiyan ,  CRESSMAN, Sonya

    IPC分类号： C12Q1/00 ,  G01N33/53 ,  C07K14/00

    CPC分类号： C07K1/1077 ,  C07K1/023 ,  C07K1/047 ,  C07K1/1072 ,  C07K14/535 ,  Y02P20/55

    摘要： The invention concerns a multiplex polymer ligation method involving water-soluble polymeric protecting groups, compositions and methods of use. The method involves synthesis of a first compound bearing one or more water-soluble polymeric protecting groups followed by replacing one or more of the water-soluble protecting groups with a chemical adduct of interest. Multiple different compounds derived from the first compound can be readily prepared by splitting the first compound into first and second reaction systems, followed by replacing one or more of the water-soluble polymeric protecting groups of the first reaction system with a first chemical adduct, and replacing one or more of the water-soluble polymeric protecting groups of the second reaction system with a second chemical adduct of interest, where the first and second chemical adducts are different. Also provided are kits useful for carrying out the multiplex polymer ligation methods of the invention.