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1.发明公开METHOD OF INTRODUCTION AND REMOVAL OF HIGH CONCENTRATIONS OF CRYOPROTECTANTS BY VASCULAR PERFUSION 审中-公开程序由血管灌流冷冻保护产品引入和去除高含量
公开(公告)号：EP3177140A1
公开(公告)日：2017-06-14
申请号：EP15828048.7
申请日：2015-07-28
IPC分类号： A01N1/02
CPC分类号： A61B17/025 , A01N1/0247 , A61B2017/0275 , A61F2/4607 , A61F2/4609 , A61F2002/4619 , A61F2002/4623
摘要： Surgical tools which are used to separate from one another a femoral cup and an acetabular ball in an implanted hip replacement prosthesis. At its distal end, elements of the surgical tool engage the femoral cup and the acetabular cup. When proximal handles of the tool are squeezed toward one another, the engagement elements move away from one another. Thus, a surgeon is able to separate the femoral cup and the acetabular ball from one another without pulling the acetabular cup away from the acetabulum or the femoral cup and/or femoral implant away from the femur, thereby accomplishing the separation without disrupting any bone ingrowth.
摘要翻译： 本发明涉及通过冷冻或玻璃化血管化组织和器官的保存; 到器官和组织的冷冻保存或银行业务; 并且组织和器官灌注冷冻保护剂（因此称为冷冻保护剂）。
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公开(公告)号：EP1675457A2
公开(公告)日：2006-07-05
申请号：EP04784413.9
申请日：2004-09-16
发明人： FAHY, Gregory, M. , WOWK, Brian
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0284
摘要： Methods and compositions are provided for the introduction and washout of vitrifiable concentrations of cryoprotectant in organs and tissues. The methods comprise cooling the organ to below -10°C by perfusion with a solution having a freezing point below -10°C, a temperature from -10 to -40°C, and a tonicity from 1.1 to 2.0 times isotonic, after previous perfusion with said solution for a time insufficient for approximate osmotic equilibration of the organ with the solution. The methods further comprise increasing the concentration of cryoprotectant further at a temperature from -10 to -40°C to prepare the organ or tissue for vitrification. The methods further comprise cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant that is the same as or less than the concentration used for vitrification, without the addition of an osmotic buffering agent. Rewarming is accomplished either by rapid (>1 °C/min, and preferably -.2-20°C/min) elevation of arterial perfusate temperature from below -20°C to above -15°C during continuous perfusion of the organ or by perfusing the organ with pre-warmed arterial perfusate at >--15°C. Extraordinarily effective multicomponent compositions are also provided for the process, particularly involving a vitrification solution whose warming rate after vitrification can be
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公开(公告)号：EP1638393A2
公开(公告)日：2006-03-29
申请号：EP04755004.1
申请日：2004-06-10
发明人： WOWK, Brian , IAROCCI, Michael
IPC分类号： A01N1/00
CPC分类号： A01N1/0263 , A01N1/02 , A01N1/0257 , A01N1/0268 , F17C2203/0304 , F17C2203/0617 , F17C2203/0629 , F17C2203/0636 , F17C2203/0646 , F17C2203/0648 , F17C2205/0149 , F17C2227/0337 , F17C2250/0439
摘要： The present invention provides devices and methods for the cryogenic storage of biological material. Devices of the invention are useful for storing material at a cryogenic temperature. The devices include a temperature chamber defined by a thermally-conductive container and at least one layer of thermal insulation surrounding the thermally-conductive container. Some embodiments utilize one or more heat sources thermally connected to the thermally conductive container. Other embodiments are arranged so that no net flow of heat occurs from the temperature chamber when the temperature chamber is at a set target temperature. Also provided are methods of using the devices.
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5.发明公开POLYGLYCEROL AND LACTOSE COMPOSITIONS FOR THE PROTECTION OF LIVING SYSTEMS FROM STATES OF REDUCED METABOLISM 有权一种用于细胞，组织和器官的Preservierung过程
公开(公告)号：EP1476014A2
公开(公告)日：2004-11-17
申请号：EP03737581.3
申请日：2003-01-31
发明人： FAHY, Greg , WU, Ju
IPC分类号： A01N1/02
CPC分类号： C09K3/18 , A01N1/02 , A01N1/0221 , C09K5/20
摘要： Polyglycerol, lactose, and a combination of polyglycerol and lactose are effective at preserving cells, tissues, and organs from damage due to hypothermic, ischemic, or other metabolic impairment, and a mixture of polyglycerol plus lactose is particularly useful for the hypothermic storage of cells, tissues, and organs. The mixture of polyglycerol and lactose can be further improved by the addition of chondroitin sulfate, chlorpromazine, calcium, citrate, glutathione, adenine, glucose, magnesium, and a pH buffer.
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公开(公告)号：EP1422999B1
公开(公告)日：2018-01-24
申请号：EP02752596.3
申请日：2002-07-25
CPC分类号： A01N1/0221 , A01N1/02
摘要： This invention relates generally to cryopreservation and a method for preventing injury caused by cooling and warming of tissue and for reducing the toxicity of vitrification solutions. The present method achieves reduction or elimination of injury by increasing the tonicity of the medium to greater than isotonic prior to cooling. The method was developed by attempting to simulate without freezing, the events that take place during freezing living cells and/or tissue. A further benefit of the method is that, since the cryopreservation medium is hypertonic, it can be diluted to a more extreme degree in one step once the system is rewarmed, without engendering the degree of cell swelling that would attend the same dilution factor when diluting an isotonic cryopreservation medium.
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7.发明公开POLYGLYCEROL AND LACTOSE COMPOSITIONS FOR THE PROTECTION OF LIVING SYSTEMS FROM STATES OF REDUCED METABOLISM 有权生活系统免受保护聚甘油LACTOSEZUSAMMENSETZUNGEN降低代谢条件
公开(公告)号：EP1476014A4
公开(公告)日：2006-09-06
申请号：EP03737581
申请日：2003-01-31
CPC分类号： C09K3/18 , A01N1/02 , A01N1/0221 , C09K5/20
摘要： Polyglycerol, lactose, and a combination of polyglycerol and lactose are effective at preserving cells, tissues, and organs from damage due to hypothermic, ischemic, or other metabolic impairment, and a mixture of polyglycerol plus lactose is particularly useful for the hypothermic storage of cells, tissues, and organs. The mixture of polyglycerol and lactose can be further improved by the addition of chondroitin sulfate, chlorpromazine, calcium, citrate, glutathione, adenine, glucose, magnesium, and a pH buffer.
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公开(公告)号：EP1115281B9
公开(公告)日：2006-05-10
申请号：EP99954636.9
申请日：1999-09-21
发明人： WOWK, Brian , FAHY, Gregory, M.
摘要： Surprising new combinations of previously-known and novel cryoprotectants are provided that yield superior recovery of function and viability of living systems after exposure to and removal from said systems. These and related combinations are useful for cryopreservation by vitrification, freezing, supercooling, freezing point depression, or cold storage. It is disclosed that, contrary to current opinion, ideal solutions for cryopreservation are those that vitrify 'poorly' (i.e., at higher rather than at lower concentrations). Contrary to expectation, by using relatively 'poor' vitrifiers, the water content of the solution is reduced at the concentration needed to vitrify, but the water availability within the solution is believed to be paradoxically increased, thereby increasing viability. A novel method for understanding and predicting non-specific cryoprotectant toxicity is provided based on a new definition of cryoprotectant 'concentration', which is the number of water molecules per polar group on penetrating cryoprotectants. Compositions are provided that vitrify at relatively high concentrations, yet surprisingly also devitrify slowly on warming. Databases of novel vitrification/devitrification and toxicity data are provided that allow the ordinary practitioner of the art to select specific solutions or obvious solution variants to meet the user's specific cryopreservation needs.
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公开(公告)号：EP1115281B1
公开(公告)日：2005-12-21
申请号：EP99954636.9
申请日：1999-09-21
发明人： WOWK, Brian , FAHY, Gregory, M.
摘要： Surprising new combinations of previously-known and novel cryoprotectants are provided that yield superior recovery of function and viability of living systems after exposure to and removal from said systems. These and related combinations are useful for cryopreservation by vitrification, freezing, supercooling, freezing point depression, or cold storage. It is disclosed that, contrary to current opinion, ideal solutions for cryopreservation are those that vitrify 'poorly' (i.e., at higher rather than at lower concentrations). Contrary to expectation, by using relatively 'poor' vitrifiers, the water content of the solution is reduced at the concentration needed to vitrify, but the water availability within the solution is believed to be paradoxically increased, thereby increasing viability. A novel method for understanding and predicting non-specific cryoprotectant toxicity is provided based on a new definition of cryoprotectant 'concentration', which is the number of water molecules per polar group on penetrating cryoprotectants. Compositions are provided that vitrify at relatively high concentrations, yet surprisingly also devitrify slowly on warming. Databases of novel vitrification/devitrification and toxicity data are provided that allow the ordinary practitioner of the art to select specific solutions or obvious solution variants to meet the user's specific cryopreservation needs.
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公开(公告)号：EP1115281A1
公开(公告)日：2001-07-18
申请号：EP99954636.9
申请日：1999-09-21
发明人： WOWK, Brian , FAHY, Gregory, M.
IPC分类号： A01N1/02
摘要： Surprising new combinations of previously-known and novel cryoprotectants are provided that yield superior recovery of function and viability of living systems after exposure to and removal from said systems. These and related combinations are useful for cryopreservation by vitrification, freezing, supercooling, freezing point depression, or cold storage. It is disclosed that, contrary to current opinion, ideal solutions for cryopreservation are those that vitrify 'poorly' (i.e., at higher rather than at lower concentrations). Contrary to expectation, by using relatively 'poor' vitrifiers, the water content of the solution is reduced at the concentration needed to vitrify, but the water availability within the solution is believed to be paradoxically increased, thereby increasing viability. A novel method for understanding and predicting non-specific cryoprotectant toxicity is provided based on a new definition of cryoprotectant 'concentration', which is the number of water molecules per polar group on penetrating cryoprotectants. Compositions are provided that vitrify at relatively high concentrations, yet surprisingly also devitrify slowly on warming. Databases of novel vitrification/devitrification and toxicity data are provided that allow the ordinary practitioner of the art to select specific solutions or obvious solution variants to meet the user's specific cryopreservation needs.
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