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    Improved AAV vectors produced in insect cells
    8.
    发明公开
    Improved AAV vectors produced in insect cells 有权
    Verbesserte AAV Vektoren，insektenzellen的hergestellt

    公开(公告)号：EP2311967A2

    公开(公告)日：2011-04-20

    申请号：EP10186085.6

    申请日：2006-10-19

    申请人： Amsterdam Molecular Therapeutics B.V.

    发明人： Urabe, Masashi ,  Ozawa, Keiya ,  Haast, Saskia Jacoba Petronella ,  Hermens, Wilhelmus Theodorus Johannes Maria Christ

    IPC分类号： C12N15/864 ,  C12N15/866 ,  C12N15/35 ,  C12N5/10

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14144 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151

    摘要： The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rcp52 or a Rcp40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles.

    摘要翻译： 本发明涉及在昆虫细胞中产生腺相关病毒载体。 因此，昆虫细胞包含编码腺相关病毒（AAV）衣壳蛋白的第一核苷酸序列，由此AAV VP1衣壳蛋白的翻译起始密码子是非ATG，次最初始密码子。 昆虫细胞还包含第二核苷酸序列，其包含至少一个AAV反向末端重复（ITR）核苷酸序列; 包含与用于在昆虫细胞中表达的表达控制序列可操作地连接的Rcp52或Rcp40编码序列的第三核苷酸序列; 以及包含与表达控制序列可操作地连接以在昆虫细胞中表达的Rep78或Rep68编码序列的第四核苷酸序列。 本发明还涉及具有改变的病毒衣壳蛋白的比率的腺相关病毒载体，其提供病毒颗粒的改善的感染性。

    Improved AAV vectors produced in insect cells
    9.
    发明公开
    Improved AAV vectors produced in insect cells 审中-公开
    改进的AAV载体在昆虫细胞中产生

    公开(公告)号：EP2311966A2

    公开(公告)日：2011-04-20

    申请号：EP10185875.1

    申请日：2006-10-19

    申请人： Amsterdam Molecular Therapeutics B.V.

    发明人： Urabe, Masashi ,  Ozawa, Keiya ,  Haast, Saskia Jacoba Petronella ,  Hermens, Wilhelmus Theodorus Johannes Maria Christ

    IPC分类号： C12N15/864 ,  C12N15/866 ,  C12N15/35 ,  C12N5/10

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14144 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151

    摘要： The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rcp52 or a Rcp40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles.

    摘要翻译： 本发明涉及在昆虫细胞中产生腺伴随病毒载体。 昆虫细胞因此包含编码腺伴随病毒（AAV）衣壳蛋白的第一核苷酸序列，其中用于翻译AAV VP1衣壳蛋白的起始密码子是非ATG，次优起始密码子。 昆虫细胞还包含第二核苷酸序列，其包含至少一个AAV反向末端重复序列（ITR）核苷酸序列; 第三核苷酸序列，其包含与表达控制序列可操作连接的Rcp52或Rcp40编码序列，用于在昆虫细胞中表达; 和第四核苷酸序列，其包含与表达控制序列可操作连接的用于在昆虫细胞中表达的Rep78或Rep68编码序列。 本发明进一步涉及具有改变的病毒衣壳蛋白比例的腺伴随病毒载体，其提供改善的病毒颗粒感染性。