公开(公告)号：EP2102351A1

公开(公告)日：2009-09-23

申请号：EP07851495.7

申请日：2007-12-14

Applicant: Biofuelchem Co., Ltd.

Inventor： PAPOUTSAKIS, Eleftherios Terry ,  LEE, Sang Yup ,  PARK, Jin Hwan

IPC: C12P7/16 ,  C12N1/21

CPC classification number: C12P7/16 ,  Y02E50/10

Abstract: The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol.

公开(公告)号：EP2222845A4

公开(公告)日：2012-03-07

申请号：EP08865113

申请日：2008-12-22

Applicant: KOREA ADVANCED INST SCI & TECH ,  BIOFUELCHEM CO ,  GS CALTEX CORP

Inventor： LEE SANG YUP ,  JANG YU-SIN ,  LEE JIN YOUNG ,  JUNG KWANG SEOP ,  KIM JAE HYUN

IPC: C12N9/04 ,  C12N9/10 ,  C12P7/06 ,  C12P7/16

CPC classification number: C12P7/065 ,  C12N9/0006 ,  C12N9/13 ,  C12P7/16 ,  Y02E50/10 ,  Y02E50/17

公开(公告)号：EP2109675A4

公开(公告)日：2012-02-29

申请号：EP08712435

申请日：2008-02-11

Applicant: BIOFUELCHEM CO LTD

Inventor： LEE SANG YUP ,  PAPOUTSAKIS ELEFTHERIOS TERRY ,  JANG YU-SIN

IPC: C12N15/81 ,  C12P7/16

CPC classification number: C12P7/16 ,  C12N15/81 ,  Y02E50/10

公开(公告)号：EP2481793A4

公开(公告)日：2013-07-10

申请号：EP10819056

申请日：2010-09-21

Applicant: KOREA ADVANCED INST SCI & TECH ,  BIOFUELCHEM CO ,  GS CALTEX CORP

Inventor： LEE SANG YUP ,  JANG YU-SIN ,  LEE JIN YOUNG ,  LEE JOUNG MIN ,  PARK JIN HWAN

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/16

CPC classification number: C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

Abstract: The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

Abstract translation: 本发明涉及具有增加的生产丁醇的能力，并使用相同的生产丁醇的方法的重组微生物。 更具体地，本发明涉及重组微生物谁产生丁醇能力通过他们的代谢网络的操作什么增加，并使用相同的生产丁醇的方法。 具有增加的生产丁醇能力的重组微生物包含一个缺失的基因，酶的编码的并将乙酰辅酶A在宿主微生物于乙酸盐具有基因编码的酶在乙酰CoA并参与和丁酰辅酶A生物合成途径。 通过操纵微生物的代谢通量得到的重组微生物能够选择性地产生丁醇具有高效率，并且因此作为用于生产工业溶剂和运输燃料的微生物有用。

公开(公告)号：EP2481793A2

公开(公告)日：2012-08-01

申请号：EP10819056.2

申请日：2010-09-21

Applicant: Korea Advanced Institute of Science and Technology ,  Biofuelchem Co. ,  GS Caltex Corporation

Inventor： LEE, Sang Yup ,  JANG, Yu-Sin ,  LEE, Jin Young ,  LEE, Joung Min ,  PARK, Jin Hwan

IPC: C12N1/21 ,  C12P7/16 ,  C12N15/52

CPC classification number: C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

Abstract: The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

公开(公告)号：EP2222845A2

公开(公告)日：2010-09-01

申请号：EP08865113.8

申请日：2008-12-22

Applicant: Korea Advanced Institute of Science and Technology ,  Biofuelchem Co. ,  Gs Caltex Corporation

Inventor： LEE, Sang Yup ,  JANG, Yu-Sin ,  LEE, Jin Young ,  JUNG, Kwang Seop ,  KIM, Jae Hyun

IPC: C12N15/09

CPC classification number: C12P7/065 ,  C12N9/0006 ,  C12N9/13 ,  C12P7/16 ,  Y02E50/10 ,  Y02E50/17

Abstract: The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.

公开(公告)号：EP2094844A4

公开(公告)日：2010-01-06

申请号：EP07851496

申请日：2007-12-14

Applicant: BIOFUELCHEM CO LTD

Inventor： LEE SANG YUP ,  PARK JIN HWAN ,  PAPOUTSAKIS ELEFTHERIOS TERRY

IPC: C12N15/00

CPC classification number: C12P7/16 ,  Y02E50/10

公开(公告)号：EP2481793B1

公开(公告)日：2017-08-30

申请号：EP10819056.2

申请日：2010-09-21

Applicant: Korea Advanced Institute of Science and Technology ,  Biofuelchem Co. ,  GS Caltex Corporation

Inventor： LEE, Sang Yup ,  JANG, Yu-Sin ,  LEE, Jin Young ,  LEE, Joung Min ,  PARK, Jin Hwan

IPC: C12N1/21 ,  C12P7/16 ,  C12N15/52

CPC classification number: C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

Abstract: The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

Abstract translation: 本发明涉及具有提高的丁醇生产能力的重组微生物以及使用该微生物生产丁醇的方法。 更具体地说，本发明涉及通过操作其代谢网络来增加丁醇生产能力的重组微生物，以及使用该微生物生产丁醇的方法。 具有增加的丁醇生产能力的重组微生物包括在编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中缺失编码将乙酰辅酶A转化为乙酸的酶的基因。 通过操作微生物的代谢流而获得的重组微生物能够高效选择性地生产丁醇，因此可用作用于生产工业溶剂和运输燃料的微生物。

公开(公告)号：EP2102351A4

公开(公告)日：2010-01-06

申请号：EP07851495

申请日：2007-12-14

Applicant: BIOFUELCHEM CO LTD

Inventor： PAPOUTSAKIS ELEFTHERIOS TERRY ,  LEE SANG YUP ,  PARK JIN HWAN

IPC: C12P7/16 ,  C12N1/21

CPC classification number: C12P7/16 ,  Y02E50/10

公开(公告)号：EP2109675A1

公开(公告)日：2009-10-21

申请号：EP08712435.0

申请日：2008-02-11

Applicant: Biofuelchem Co., Ltd.

Inventor： LEE, Sang Yup ,  PAPOUTSAKIS, Eleftherios Terry ,  JANG, Yu-Sin

IPC: C12N15/81

CPC classification number: C12P7/16 ,  C12N15/81 ,  Y02E50/10

Abstract: Disclosed herein are a method for producing butanol in yeast having the ability to biosynthesize butanol using butyryl-CoA as an intermediate, the method comprises producing butyryl-CoA in yeast having a CoAT (acetyl-CoA:butyryl- CoA CoA-transferase)-encoding gene introduced thereinto, through various pathways, and then converting the produced butyryl-CoA to butanol.