METHOD FOR FORMING CELLULOSE
    3.
    发明公开
    METHOD FOR FORMING CELLULOSE 有权
    用于生产纤维素

    公开(公告)号:EP1304337A4

    公开(公告)日:2004-07-07

    申请号:EP01947969

    申请日:2001-07-11

    IPC分类号: C08B15/02 C08B15/08 C08B1/00

    摘要: A method for forming II-type cellulose, which comprises introducing together a slurry having water and a I-type cellulose dispersed therein and water in the state of high temperature and high pressure to a tubular reactor (28), contacting the I-type cellulose with a hot water in a supercritical state or subcritical state for a predetermined time in the tubular reactor (28), to thereby decompose a part of the hydrogen bonds in the I-type cellulose and dissolve the I-type cellulose into the water in a supercritical state or subcritical state, cooling a cellulose solution discharged from the tubular reactor (28) in a cooler (30) to room temperature or lower, followed by allowing to stand, to thereby precipitate crystals of II-type cellulose having a molecular weight lower than that of the I-type cellulose, and separating the resultant II-type cellulose from a solvent. The method allows the preparation of pure II-type cellulose by the use of a simple process.

    METHOD OF DETERMINING BASE SEQUENCE OF SINGLE NUCLEIC ACID MOLECULE
    4.
    发明公开
    METHOD OF DETERMINING BASE SEQUENCE OF SINGLE NUCLEIC ACID MOLECULE 有权
    一种用于确定单一分子NUCLEIC的碱基序列

    公开(公告)号:EP1182267A4

    公开(公告)日:2004-09-15

    申请号:EP01917739

    申请日:2001-03-30

    IPC分类号: C12Q1/68

    摘要: A method of determining the base sequence of a nucleic acid by detecting a single dye molecule, which involves the following steps: (1) fixing to a solid surface a nucleic acid molecule or a primer having a sequence complementary to a part of the sequence thereof; (2) annealing the above nucleic acid molecule or primer with the corresponding primer or nucleic acid molecule; (3) supplying a solution containing DNA polymerase and a dye-labeled dNTP or RNA polymerase and a dye-labeled NTP to the fixed nucleic acid molecule and reacting the nucleotide with the 3'-end of the primer to thereby allowing the polymerase to incorporate the nucleotide forming a base pair with the base opposite to the reaction site; (4) detecting the dye-labeled dNTP or NTP thus bonded; (5) disrupting the dye molecule of the bonded dye-labeled dNTP or NTP; (6) repeating the procedures of the steps (3) to (5) while successively changing the type of the dye-labeled dNTP or NTP so as to bond the dNTP or NTP forming a base pair with the nucleotide of the nucleic acid molecule; and (7) determining the base sequence of the nucleic acid molecule based on the dNTP or NTP thus bonded.