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    METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS THROUGH PROTEASE MANIPULATION
    3.
    发明公开
    METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS THROUGH PROTEASE MANIPULATION 审中-公开
    通过蛋白酶操作控制代谢途径中的通量的方法

    公开(公告)号：EP3219796A1

    公开(公告)日：2017-09-20

    申请号：EP17163812.5

    申请日：2011-08-31

    申请人： Greenlight Biosciences, Inc. ,  The Board of Trustees of the Leland Stanford Junior University

    发明人： SWARTZ, James R.

    IPC分类号： C12N9/50 ,  C12N15/52 ,  C12N15/70 ,  C12P7/42 ,  C12P19/30

    摘要： The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.

    摘要翻译： 本文描述的实施方案涉及细胞和用于制备细胞的方法，其可以通过改变竞争感兴趣途径的底物或产物的酶而用作生物催化剂，使得靶酶对位点特异性蛋白酶敏感， 蛋白酶被表达但在细胞中重新定位到它不与完整细胞中的靶酶接触的位置。 细胞裂解后，蛋白酶接触目标酶，然后通过蛋白酶切割使其失活。

    ENGINEERED PROTEINS WITH A PROTEASE CLEAVAGE SITE
    4.
    发明公开
    ENGINEERED PROTEINS WITH A PROTEASE CLEAVAGE SITE 审中-公开
    手工蛋白质麻醉师EINER PROTEASESCHNITTSTELLE

    公开(公告)号：EP3030671A2

    公开(公告)日：2016-06-15

    申请号：EP14752728.7

    申请日：2014-08-05

    申请人： Greenlight Biosciences, Inc.

    发明人： BLAKE, William Jeremy ,  CUNNINGHAM, Drew S.

    IPC分类号： C12Q1/37 ,  C12N9/10 ,  C12N9/50

    CPC分类号： C12N9/92 ,  C12N9/1022 ,  C12N9/1029 ,  C12Y202/01001 ,  C12Y203/01008 ,  C12Y503/01009

    摘要： Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.

    摘要翻译： 本文的各个方面和实施方案涉及具有至少一种可被同源蛋白酶灭活的蛋白酶识别序列的重组蛋白质和制备此类蛋白质的方法。 在一些实施方案中，提供重组磷酸葡萄糖异构酶（Pgi）蛋白。 在其它实施方案中，提供重组磷酸转乙酰酶（Pta）蛋白质。 在其它实施方案中，提供重组转酮醇酶A（TktA）蛋白。

    CONTROL OF METABOLIC FLUX IN CELL-FREE BIOSYNTHETIC SYSTEMS
    10.
    发明公开
    CONTROL OF METABOLIC FLUX IN CELL-FREE BIOSYNTHETIC SYSTEMS 审中-公开
    代谢流动的在无细胞系统合成控制

    公开(公告)号：EP3004335A1

    公开(公告)日：2016-04-13

    申请号：EP14807322.4

    申请日：2014-06-05

    申请人： Greenlight Biosciences Inc. ,  The Board of Trustees of The Leland Stanford Junior University

    发明人： SWARTZ, James, R.

    IPC分类号： C12N9/00

    CPC分类号： C12Q3/00 ,  C12N9/00

    摘要： Methods are provided for controlling metabolic flux rate in a cell-free system comprising a complex set of enzymes, to produce a desired product of a pathway of interest. In the methods of the invention, measurements of metabolic performance parameters are taken by continuous monitoring or intermittent monitoring. Based on the metabolic performance parameters, the system is modified by one or more steps comprising: (i) altering enzyme levels in the cell-free system; (ii) altering feed rate of a substrate that controls redox flux or carbon flux to the cell-free system; (iii) altering 02 addition to the cell-free system; (iv) controlling efficiency of electron transport system by altering leakage across a membrane; wherein enzymes present in the pathway of interest catalyze production of a desired product.