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    ALCOHOL DEHYDROGENASE VARIANTS
    1.
    发明公开
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    ALKOHOLDEHYDROGENASEVARIANTEN

    公开(公告)号：EP3052635A4

    公开(公告)日：2017-06-21

    申请号：EP14850119

    申请日：2014-10-03

    申请人： GENOMATICA INC

    发明人： ANDRAE STEFAN ,  KUCHINSKAS MICHAEL PATRICK ,  LI JINGYI ,  NAGARAJAN HARISH ,  PHARKYA PRITI

    IPC分类号： C12P1/00 ,  C12P5/00 ,  C12P7/18 ,  C12P7/24

    CPC分类号： C12N9/0006 ,  C12P7/18 ,  C12P7/24 ,  C12Y101/01001 ,  C12Y101/01244 ,  Y02P20/127

    摘要： Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    摘要翻译： 与未修饰的对应物相比，本文描述的是非天然NAD +依赖性醇脱氢酶（ADH），其分别能够将甲醇或乙醇分别转化为甲醛或乙醛的两倍以上。 描述了编码非天然醇脱氢酶的核酸，以及包括核酸的表达构建体，以及包含核酸或表达构建体的工程细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程化细胞，任选地包含一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括所述细胞的细胞培养组合物，用于促进产生的方法 来自细胞的目标产物或其中间体，包含目标产物或中间体的组合物，以及由目标产物或中间体制备的产物。

    RECOMBINANT CELL, AND METHOD FOR PRODUCING 1,4-BUTANEDIOL
    2.
    发明公开
    RECOMBINANT CELL, AND METHOD FOR PRODUCING 1,4-BUTANEDIOL 有权
    REKOMBINANTE ZELLE UND VERFAHREN ZUR HERSTELLUNG VON 1,4-BUTANDIOL

    公开(公告)号：EP2947143A4

    公开(公告)日：2016-07-06

    申请号：EP14741160

    申请日：2014-01-20

    申请人： SEKISUI CHEMICAL CO LTD

    发明人： FURUTANI MASAHIRO ,  UENISHI AKIHIRO ,  IWASA KOICHIRO

    IPC分类号： C12N1/21 ,  C12N1/19 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/09 ,  C12P7/18

    CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/10 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01244 ,  C12Y401/02043 ,  C12Y402/03027

    摘要： An object of the present invention is to provide a series of techniques for producing 1,4-butanediol from methanol or the like. Provided is a recombinant cell prepared by introducing a gene encoding at least one enzyme selected from the group consisting of succinate semialdehyde dehydrogenase, succinyl-CoA synthase, CoA-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA reductase, 4-hydroxybutyraldehyde dehydrogenase, and alcohol dehydrogenase, into a host cell which is a methylotroph, wherein the gene is expressed in the host cell, and the recombinant cell is capable of producing 1, 4-butanediol from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde, and formamide.

    摘要翻译： 本发明的目的是提供一系列用于从甲醇等生产1,4-丁二醇的技术。 提供了通过引入编码选自琥珀酸半醛脱氢酶，琥珀酰辅酶A合酶，辅酶A依赖性琥珀酸半醛脱氢酶，4-羟基丁酸脱氢酶，4-羟基丁酰-CoA转移酶，4 - 羟基丁酰辅酶A还原酶，4-羟基丁醛脱氢酶和醇脱氢酶转化成宿主细胞，其是甲基营养物，其中所述基因在宿主细胞中表达，并且重组细胞能够至少从至少产生1,4-丁二醇 一种选自甲烷，甲醇，甲胺，甲酸，甲醛和甲酰胺的C1化合物。

    NUCLEIC ACIDS AND VECTORS FOR USE WITH METHANOTROPHIC BACTERIA
    3.
    发明公开
    NUCLEIC ACIDS AND VECTORS FOR USE WITH METHANOTROPHIC BACTERIA 审中-公开
    用于甲烷嗜酸细菌的核酸和载体

    公开(公告)号：EP3158065A1

    公开(公告)日：2017-04-26

    申请号：EP15809220.5

    申请日：2015-06-18

    申请人： Calysta, Inc.

    发明人： SAVILLE, Renee, M. ,  SILVERMAN, Joshua ,  MINSHULL, Jeremy ,  NESS, Jon, Edward ,  LEONARD, Effendi ,  STUMPE, Jana ,  WELCH, Mark

    IPC分类号： C12N15/11

    CPC分类号： C12N15/74 ,  C12N9/0006 ,  C12N2830/002 ,  C12Y101/01244

    摘要： The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided. In one aspect, the present disclosure is directed to a non-naturally occurring nucleic acid molecule, comprising (1) a promoter that is functional in a methanotrophic bacterium, and (2) a native or altered methanol dehydrogenase (MDH) ribosomal binding sequence, provided that when the promoter is an MDH gene promoter, the nucleic acid comprises an altered MDH ribosomal binding sequence.

    摘要翻译： 本公开提供了用于甲烷营养细菌的核酸和载体。 还提供了相关的宿主细胞和使用这些核酸和载体来表达多肽或甲烷营养细菌的其他基因操作的方法。 在一个方面，本公开涉及非天然存在的核酸分子，其包含（1）在甲烷营养细菌中有功能的启动子，和（2）天然或改变的甲醇脱氢酶（MDH）核糖体结合序列， 条件是当启动子是MDH基因启动子时，核酸包含改变的MDH核糖体结合序列。

    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    8.
    发明公开
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    VERFAHREN ZUR VERBESSERUNG DER MIKROBIELLEN HERSTELLUNG VON FETTALKOHOLEN MIT BESTIMMTERLÄNGEGEGENWART VON METHANOL

    公开(公告)号：EP3074504A4

    公开(公告)日：2017-05-03

    申请号：EP14864741

    申请日：2014-11-25

    申请人： GENOMATICA INC

    发明人： OSTERHOUT ROBIN E ,  BURGARD ANTHONY P ,  PHARKYA PRITI ,  ANDRAE STEFAN

    IPC分类号： C12N1/19 ,  C12N1/11 ,  C12N1/13 ,  C12N1/15 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P7/04

    CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    摘要： The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    摘要翻译： 本发明提供了具有甲醛固定途径，甲酸盐同化途径和/或甲醇代谢途径以及脂肪醇，脂肪醛，脂肪酸或异丙醇途径的非天然存在的微生物，其中所述微生物有选择地产生 脂肪醇，特定长度的脂肪醛或脂肪酸或异丙醇。 所提供的微生物有利地增强用于产生链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的产生。 在一些方面，本发明的微生物具有选择性基因破坏或酶衰减，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明另外提供了使用上述微生物有机体来生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

    ALCOHOL DEHYDROGENASE VARIANTS
    10.
    发明公开
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    醇脱氢酶变体

    公开(公告)号：EP3052635A2

    公开(公告)日：2016-08-10

    申请号：EP14850119.0

    申请日：2014-10-03

    申请人： Genomatica, Inc.

    发明人： ANDRAE, Stefan ,  KUCHINSKAS, Michael Patrick ,  LI, Jingyi ,  NAGARAJAN, Harish ,  PHARKYA, Priti

    IPC分类号： C12P1/00 ,  C12P5/00

    CPC分类号： C12N9/0006 ,  C12P7/18 ,  C12P7/24 ,  C12Y101/01001 ,  C12Y101/01244 ,  Y02P20/127

    摘要： Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD
    + -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    摘要翻译： 与未修饰的对应物相比，本文描述的是非天然NAD +依赖性醇脱氢酶（ADH），其分别能够将甲醇或乙醇分别转化为甲醛或乙醛的两倍以上。 描述了编码非天然醇脱氢酶的核酸，以及包括核酸的表达构建体，以及包含核酸或表达构建体的工程细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进 从细胞生产目标产物或其中间体，包括目标产物或中间体的组合物，以及由目标产物或中间体制备的产物。