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1.发明公开Production of antibodies in plants with plus-sense single-stranded viral RNA vectors 审中-公开Pflanzen mit plus-senseeinzelsträngigenRNA Vektoren的产品vonAntikörpern
公开(公告)号：EP1686176A1
公开(公告)日：2006-08-02
申请号：EP05001819.1
申请日：2005-01-28
申请人： Icon Genetics AG
CPC分类号： C12N15/8251 , C07K14/59 , C12N15/8203 , C12N15/8257 , C12N15/8258
摘要： Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by
(i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or
(ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.摘要翻译： 在植物，植物组织或植物细胞中产生包含至少第一和第二蛋白质亚基的杂低聚蛋白的方法，所述方法包括在植物细胞中至少表达所述第一和所述第二蛋白质亚基（i ）向所述植物，所述植物组织或所述植物细胞提供编码至少所述第一和所述第二蛋白质亚基的正义单链RNA病毒载体，或（ii）向所述植物提供所述植物组织或所述植物细胞 第一和第二正义单链RNA病毒载体，所述第一病毒载体编码至少所述第一蛋白质亚基，所述第二病毒载体编码至少所述第二蛋白质亚基，其中至少所述第一病毒载体和所述第二病毒载体 是非竞争的病毒载体。
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公开(公告)号：EP1616959A1
公开(公告)日：2006-01-18
申请号：EP04016011.1
申请日：2004-07-07
申请人： Icon Genetics AG
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising:
(a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon,
wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon,
(b) isolating said protein of interest from said plant or said plant leaves infiltrated in step (a),
wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.摘要翻译： 通过从植物或植物叶中感兴趣的序列中表达所述感兴趣的蛋白质来产生目标蛋白质的方法，包括：（a）通过将所述植物或所述植物叶子渗入所述植物或所述植物叶子中来转染所述植物或所述植物叶子 农杆菌菌株在补充因子存在下，所述农杆菌菌株在T-DNA中含有具有编码复制子的序列部分的异源DNA序列，其中所述编码复制子的序列含有所述复制子的复制子功能所需的序列，所述序列得到 （b）从步骤（a）中渗透的所述植物或所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供有第一遗传修饰物 使得所述农杆菌菌株在缺乏所述补体因子的情况下缺陷以用所述T-DNA转染生物体。
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公开(公告)号：EP1564295A1
公开(公告)日：2005-08-17
申请号：EP04001460.7
申请日：2004-01-23
IPC分类号： C12N15/82
CPC分类号： C12N15/8216
摘要： A system for replicating or for replicating and expressing a sequence of interest in a plant, comprising:
(i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and
(ii) a helper replicon, or a precursor thereof, wherein said helper replicon is
(a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and
(b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i),
whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).摘要翻译： 一种用于复制或复制并表达植物感兴趣的序列的系统，包括：（i）RNA复制子或其前体，所述RNA复制子衍生自正义单链RNA病毒，并且包含至少一个序列 出于兴趣; 辅助复制子或其前体，其中所述辅助复制子是（a）在所述RNA复制子（i）和（b）能够表达的存在和不存在下，在所述植物中不能全身运动 在植物中，所述RNA复制子（i）的系统运动所必需的一种或多种蛋白质，其中所述RNA复制子（i）能够在所述植物中复制或复制和表达所述感兴趣的序列，但不能在所述植物中全身移动 在不存在由所述辅助复制子（ii）表达的所述一种或多种蛋白质的情况下。
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公开(公告)号：EP1563078A1
公开(公告)日：2005-08-17
申请号：EP03782219.4
申请日：2003-11-20
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8216 , C12N15/8217
摘要： A method of controlling a genetically-modified multi-cellular organism or a part thereof, comprising the following steps: (a) providing a multi-cellular organism or a part thereof, whereby cells of said multi-cellular organism or said part contain a heterologous nucleic acid, (b) causing expression of a I protein from said heterologous nucleic acid in at least some of said cells, wherein said protein is capable of (i) leaving a cell and entering other cells of said multi-cellular organism or a part thereof, (ii) causing expression of said protein in cells containing said heterologous nucleic acid, and optionally (iii) controlling a cellular process of interest.
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公开(公告)号：EP1527185A1
公开(公告)日：2005-05-04
申请号：EP03784146.7
申请日：2003-08-01
申请人： Icon Genetics AG
CPC分类号： C12N15/8214
摘要： A process of generating transgenic plants or plant cells transformed on their plastome, comprising (a) introducing into plant plastids a first DNA molecule and a second DNA molecule, wherein said first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and said second DNA molecule contains a second region homologous to a region of the plastome for directing plastome integration and a second sequence of interest, whereby a sequence segment of said first sequence of interest is homologous to a sequence segment of said second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby said integration sequence contains at least a portion of said first and at least a portion of said second sequence of interest as a continuous sequence.
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公开(公告)号：EP1423524A2
公开(公告)日：2004-06-02
申请号：EP02767450.6
申请日：2002-08-28
申请人： Icon Genetics AG
发明人： DOROKHOV, Yurii , SKURAT, Eugene , KLIMYUK, Victor , GLEBA, Yuri
CPC分类号： C12N15/8221 , C12N15/8203 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein or polypeptide of interest in a plant or in plant is provided, comprising: (i) transforming or transfecting a plant of plant cells with a nucleotide sequence having a coding region encoding a fusion protein comprising the protein or polypeptide of interest, a signal peptide functional for targeting said fusion protein to the apoplast, and a polypeptide capable of binding the fusion protein to a cell wall component, (ii) enriching cell wall components having expressed and bound fusion protein, and separating the protein or polypeptide of interest or a protein comprising the protein or polypeptide interest.
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7.发明公开
公开(公告)号：EP1385968A1
公开(公告)日：2004-02-04
申请号：EP02730072.2
申请日：2002-03-27
CPC分类号： C07K14/005 , C12N15/8216 , C12N2770/40022
摘要： This invention discloses a biologically safe process of causing amplification and/or expression of one or more nucleic acid sequences of interest in a plant, plant tissue, plant cell or cell culture, characterized in that a plant cell is provided with at least two precursor vectors designed for undergoing processing by site-specific recombination in said cell, whereby due to said processing said plant cell is endowed with at least one replicon which provide(s) for said amplification and/or expression.
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8.发明公开
公开(公告)号：EP1364034A1
公开(公告)日：2003-11-26
申请号：EP02702375.3
申请日：2002-02-27
申请人： Icon Genetics AG
发明人： KLIMYUK, Victor , BENNING, Gregor , GLEBA, Yuri
CPC分类号： C12N15/8222
摘要： The invention describes a method of controlling a biochemical process or a biochemical cascade in plants utilizing a process of interaction between a heterologous DNA sequence in a transgenic plant, on one side, and a heterologous DNA sequence in a plant viral transfection vector, on the other. Optionally, the process of interaction further involves a low molecular weight component. The process of interaction makes the infection with a viral transfection vector a gene-'switch' which switches on a biochemical process or cascade of interest via various reactions such as nucleic acid recombination, replication, transcription, restriction, translation, protein folding, assembly, targeting, posttranslational processing, or enzymatic reaction. Further a process for producing a product in a transgenic plant and kit of parts for such a process is provided.
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公开(公告)号：EP1349947A2
公开(公告)日：2003-10-08
申请号：EP02710005.6
申请日：2002-01-14
申请人： Icon Genetics AG
发明人： HERZ, Stefan , KLAUS, Sebastian , EIBL, Christian , MÜHLBAUER, Stefan , KOOP, Hans-Ulrich , GLEBA, Yuri
CPC分类号： C12N15/8209 , C12N15/8214
摘要： This invention discloses a novel process and vector therefore for producing a multicellular plant or plant cells having stably transformed plastids. This process comprises transforming plastids or plant cells via homologous recombination with a DNA molecule enabling DNA modification, whereby said DNA molecule comprises a fragment of a gene requiring for expression a sequence element of the host plastid. The invention also includes the use of novel selection inhibitors for plastic transformation and the possibility of performing multiple rounds of transformation without accumulation of resistance genes.
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公开(公告)号：EP1423524B1
公开(公告)日：2006-08-30
申请号：EP02767450.6
申请日：2002-08-28
申请人： Icon Genetics AG
发明人： DOROKHOV, Yurii , SKURAT, Eugene , KLIMYUK, Victor , GLEBA, Yuri
CPC分类号： C12N15/8221 , C12N15/8203 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein or polypeptide of interest in a plant or in plant is provided, comprising: (i) transforming or transfecting a plant of plant cells with a nucleotide sequence having a coding region encoding a fusion protein comprising the protein or polypeptide of interest, a signal peptide functional for targeting said fusion protein to the apoplast, and a polypeptide capable of binding the fusion protein to a cell wall component, (ii) enriching cell wall components having expressed and bound fusion protein, and separating the protein or polypeptide of interest or a protein comprising the protein or polypeptide interest.
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